Remineralization of partially demineralized dentine substrate based on a biomimetic strategy

  • Xu Zhang
  • Koon Gee Neoh
  • Chew Chong Lin
  • Anil Kishen


Dentine remineralization is clinically significant for prevention and treatment of dentine caries, root caries, and dentine hypersensitivity. However, dentine remineralization is more difficult than enamel remineralization due to the abundant presence of organic matrix in dentine. The objective of this study was to develop a biomimetic method to facilitate remineralization of demineralized dentine through phosphorylation of type I collagen in demineralized dentine using sodium trimetaphosphate. The experimental results indicated that the effect of fluoride on remineralizing dentine was limited when residual mineral crystals were lacking on the surface of demineralized dentine, whereas the phosphorylation and Ca(OH)2 pretreatment enhanced surface remineralization of the partially demineralized dentine. This biomimetic methodology resulted in favorable surface properties (i.e. highly negative charge and low interfacial free energy between substrate and aqueous medium) for crystal nucleation, and thus could be a promising method to remineralize superficially demineralized dentine lesions.


Dentinal Tubule Smear Layer Interfacial Free Energy Mineral Crystal Dentine Hypersensitivity 



The authors wish to acknowledge the funding from support from the NUS-Faculty of Dentistry DBBP research initiative.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Xu Zhang
    • 1
    • 2
  • Koon Gee Neoh
    • 3
  • Chew Chong Lin
    • 2
  • Anil Kishen
    • 4
  1. 1.School of Dentistry, Hospital of StomatologyTianjin Medical UniversityTianjin CityChina
  2. 2.Department of Restorative Dentistry, Faculty of DentistryNational University of SingaporeSingaporeSingapore
  3. 3.Department of Chemical & Biomolecular EngineeringNational University of SingaporeSingaporeSingapore
  4. 4.Discipline of Endodontics, Faculty of DentistryUniversity of TorontoTorontoCanada

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