Novel alginate based nanocomposite hydrogels with incorporated silver nanoparticles
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Alginate colloid solution containing electrochemically synthesized silver nanoparticles (AgNPs) was investigated regarding the nanoparticle stabilization and possibilities for production of alginate based nanocomposite hydrogels in different forms. AgNPs were shown to continue to grow in alginate solutions for additional 3 days after the synthesis by aggregative mechanism and Ostwald ripening. Thereafter, the colloid solution remains stable for 30 days and could be used alone or in mixtures with aqueous solutions of poly(vinyl alcohol) (PVA) and poly(N-vinyl-2-pyrrolidone) (PVP) while preserving AgNPs as verified by UV–Vis spectroscopy studies. We have optimized techniques for production of Ag/alginate microbeads and Ag/alginate/PVA beads, which were shown to efficiently release AgNPs decreasing the Escherichia coli concentration in suspensions for 99.9% over 24 h. Furthermore, Ag/hydrogel discs based on alginate, PVA and PVP were produced by freezing-thawing technique allowing adjustments of hydrogel composition and mechanical properties as demonstrated in compression studies performed in a biomimetic bioreactor.
KeywordsAlginate Nucleus Pulposus Colloid Solution Electrochemical Synthesis Alginate Solution
This work was supported by the Ministry of Education and Science of the Republic of Serbia (Grant III 45019). The authors would like to thank Dr. Maja Vukašinović-Sekulić, Faculty of Technology and Metallurgy, University of Belgrade, Serbia, for the investigation of antimicrobial activity.
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