Preparation and evaluation of oleoyl-carboxymethy-chitosan (OCMCS) nanoparticles as oral protein carriers

  • Ya Liu
  • Xiao Jie Cheng
  • Qi Feng Dang
  • Fang Kui Ma
  • Xi Guang Chen
  • Hyun Jin Park
  • Bum Keun Kim


Oleoyl-carboxymethy chitosan (OCMCS) nanoparticles based on chitosan with different molecular weights (50, 170 and 820 kDa) were prepared by self-assembled method. The nanoparticles had spherical shape, positive surface charges and the mean diameters were 157.4, 274.1 and 396.7 nm, respectively. FITC-labeled OCMCS nanoparticles were internalized via the intestinal mucosa and observed in liver, spleen, intestine and heart following oral deliverance to carps (Cyprinus carpio). Extracellular products (ECPs) of Aeromonas hydrophila as microbial antigen was efficiently loaded to form OCMCS–ECPs nanoparticles and shown to be sustained release in PBS. Significantly higher (P < 0.05) antigen-specific antibodies were detected in serum after orally immunized with OCMCS-ECPs nanoparticles than that immunized with ECPs alone and non-immunized in control group in carps. These results implied that amphiphilic modified chitosan nanoparticles had great potential to be applied as carriers for the oral administration of protein drugs.


Chitosan Zeta Potential Protein Drug Aeromonas Hydrophila Mucoadhesive Property 



This work was supported by grants from the National Natural Science Foundation of China (NSFC, 81071274), International S & T Cooperation Program of China (ISTCP, S2011GR0092).


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Ya Liu
    • 1
  • Xiao Jie Cheng
    • 1
  • Qi Feng Dang
    • 1
  • Fang Kui Ma
    • 1
  • Xi Guang Chen
    • 1
  • Hyun Jin Park
    • 2
  • Bum Keun Kim
    • 3
  1. 1.College of Marine Life ScienceOcean University of ChinaQingdaoPeople’s Republic of China
  2. 2.College of Life Sciences and BiotechnologyKorea UniversitySeoulKorea
  3. 3.Korea Food Research InstituteSeoulKorea

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