Direct cytotoxicity evaluation of 63S bioactive glass and bone-derived hydroxyapatite particles using yeast model and human chondrocyte cells by microcalorimetry
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In this study, the cytotoxicity evaluation of prepared 63S bioactive glass and bone-derived hydroxyapatite particles with yeast and human chondrocyte cells was carried out using isothermal micro-nano calorimetry (IMNC), which is a new method for studying cell/biomaterial interactions. Bioactive glass particles were made via sol–gel method and hydroxyapatite was obtained from bovine bone. Elemental analysis was carried out by XRF and EDXRF. Amorphous structure of the glass and completely crystalline structure of HA were detected by XRD analysis. Finally, the cytotoxicity of bioactive glass and bone-derived HA particles with yeast and cultured human chondrocyte cells was evaluated using IMNC. The results confirmed the viability, growth and proliferation of human chondrocyte cells in contact with 63S bioactive glass, and bone-derived HA particles. Also the results indicated that yeast model which is much easier to handle, can be considered as a good proxy and can provide a rapid primary estimate of the ranges to be used in assays involving human cells. All of these results confirmed that IMNC is a convenient method which caters to measuring the cell-biomaterial interactions alongside the current methods.
KeywordsBioactive Glass Microcalorimetry Yeast Model Yeast Peptone Dextrose Chondrocyte Cell
The authors are grateful to professor Uwe Pieles (FHNW, Muttenz) for superb technical assistance.
- 2.Zhang H, Ye XJ, Li JS. Preparation and biocompatibility evaluation of apatite/wollastonite-derived porous bioactive glass ceramic scaffolds. Biomed Mater. 2009;4:353–9.Google Scholar
- 4.Hanks CT, Wataha JC, Sun Z. In vitro models of biocompatibility: a review. Dent Mater. 1996;12:186–93.Google Scholar
- 8.James AM. Thermal and energetic studies of cellular biological systems. Bristol: Wright; 1987. p. 147–66.Google Scholar
- 9.Beezer AE. Biological microcalorimetry. London: Academic Press; 1980.Google Scholar
- 11.Daniels AU, Wirz D, Trampuz A. U.S. Patent No. 0317859 (2009).Google Scholar
- 14.Kemp RB. Hand book of thermal analysis and calorimetry. Amsterdam: Elsevier; 1999.Google Scholar
- 19.Forsburg SL. The yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe: models for cell biology research. Gravit Space Biol. 2005;18:3–10.Google Scholar
- 29.Sandstrom B, Walter P. Role of trace elements for health promotion and disease prevention. Nutr Res. 1998;23:1745.Google Scholar
- 38.Nair MB, Varma HK, Kumary TV, Babu S, John A. Cell interaction studies with novel bioglass coated hydroxyapatite porous blocks. Trends Biomater Artif Organs. 2006;19:108–14.Google Scholar