Poly(ε-caprolactone) and poly(d,l-lactic acid-co-glycolic acid) scaffolds used in bone tissue engineering prepared by melt compression–particulate leaching method
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Porous bioresorbable polymers have been widely used as scaffolds in tissue engineering. Most of the bioresorbable scaffolds are aliphatic polyesters and the methods employed to prepare the porous morphology may vary. This work describes and evaluates the in vitro degradation of porous and dense scaffolds of poly(ε-caprolactone) (PCL) and poly(d,l-lactic acid-co-glycolic acid) (50/50) (PLGA50) prepared by particulate leaching-melt compression process. Biological evaluation was carried out using osteoblast cell cultures. The results showed an autocatalytic effect on the dense samples. Osteoblasts presented intermediate adhesion and the cell morphology on the surface of these materials was dispersed, which indicated a good interaction of the cells with the surface and the material.
KeywordsPLLA Porous Scaffold Salt Particle Average Molar Mass PLGA50 Scaffold
This work was supported by the Brazilian National Council for Scientific and Technological Development (CNPq) (Grant 141582).
- 1.Gloria A, De Santis R, Ambrosio L. Polymer-based composite scaffolds for tissue engineering. J Appl Biomater Biomech. 2010;8(2):57–67.Google Scholar
- 8.Eglin D, Alini M. Degradable polymeric materials for osteosynthesis: tutorial. Eur Cell Mater. 2008;16:80–91.Google Scholar
- 11.Mikos A, Temenoff J. Formation of highly porous biodegradable scaffolds for tissue engineering. Electron J Biotechnol. 2000;3:114–9. http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/427. Accessed 24 June 2011.
- 22.van Eijk F, Saris DB, Creemers LB, Riesle J, Willems WJ, van Blitterswijk CA, Verbout AJ, Dhert WJ. The effect of timing of mechanical stimulation on proliferation and differentiation of goat bone marrow stem cells cultured on braided PLGA scaffolds. Tissue Eng. 2008;14(8):1425–33.CrossRefGoogle Scholar