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A novel method for the fabrication of homogeneous hydroxyapatite/collagen nanocomposite and nanocomposite scaffold with hierarchical porosity

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Abstract

Homogeneous nanocomposites composed of hydroxyapatite (HAp) and collagen were synthesized using a novel in situ precipitation method through dual template-driven. The morphological and componential properties of nanocomposites were investigated. The HAp particulates, in sizes of about 50–100 nm, were distributed homogeneously in the organic collagen hydrogel. Highly magnified TEM observation showed that HAp inorganic particles were composed of fine sub-particles (2–5 nm) without regular crystallographic orientation. Based on these homogeneous nanocomposites, a novel HAp/collagen nanocomposite scaffold with hierarchical porosity was prepared by multilevel freeze-drying technique. Compared to other conventional scaffolds for tissue engineering, this novel in situ method endows synthesized composite scaffolds with unique morphology—ultrafine HAp particles dispersed homogenously in collagen at nano level and the foam scaffold with hierarchical pore structures. The mechanical performance increased obviously compared with neat collagen. These results provided an efficient approach toward new biomimetic tissue scaffold for the biomedical applications with enhanced intensity/bioactivity and controlled resorption rates. This novel method, we expect, will lead to a wide application in many other hydrogel systems and may be useful for fabrication of various homogeneous inorganic/organic nanocomposites.

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References

  1. Shin H, Jo S, Mikos AG. Biomimetic materials for tissue engineering. Biomaterials. 2003;24:4353–64.

    Article  CAS  Google Scholar 

  2. Sergey VD, Matthias E. Biological and medical significance of calcium phosphates. Angew Chem Int Ed. 2002;41:3130–46.

    Article  Google Scholar 

  3. Murugan R, Ramakrishna S. Development of nanocomposites for bone grafting. Compos Sci Technol. 2005;65:2385–406.

    Article  CAS  Google Scholar 

  4. Kim HW, Jonathan CK, Hyoun EK. Hydroxyapatite and gelatin composite foams processed via novel freeze-drying and crosslinking for use as temporary hard tissue scaffolds. J Biomed Mater Res. 2004;72A:136–45.

    Article  Google Scholar 

  5. Yin YJ, Zhao F, Song XF, Yao KD, Lu WW, Leong JC. Preparation and characterization of hydroxyapatite/chitosan–gelatin network composite. J Appl Polym Sci. 2000;77:2929–38.

    Article  CAS  Google Scholar 

  6. Wei GB, Peter XM. Structure and properties of nano-hydroxyapatite/polymer composite scaffolds for bone tissue engineering. Biomaterials. 2004;25:4749–57.

    Article  CAS  Google Scholar 

  7. Zhang W, Liao SS, Cui FZ. Hierarchical self-assembly of nano-fibrils in mineralized collagen. Chem Mater. 2003;15:3221–6.

    Article  CAS  Google Scholar 

  8. Taguchi T, Kishida A, Akashi M. Apatite formation on/in hydrogel matrices using an alternate soaking process. II. Effect of swelling ratios of poly(vinyl alcohol) hydrogel matrices on apatite formation. J Biomater Sci. 1999;10:331–9.

    Article  CAS  Google Scholar 

  9. Doi Y, Horiguchi T. Formation of apatite–collagen complexes. J Biomed Mater Res. 1996;31:43–9.

    Article  CAS  Google Scholar 

  10. Kischi M, Itoh S. Self-organization mechanism in a bone-like hydroxyapatite/collagen nanocomposite synthesized in vitro and its biological reaction in vivo. Biomaterials. 2001;22:1705–11.

    Article  Google Scholar 

  11. Song J, Saiz E, Bertozzi CR. A new approach to mineralization of biocompatible hydrogel scaffolds: an efficient process toward 3-dimensional bonelike composites. J Am Chem Soc. 2003;125:1236–43.

    Article  CAS  Google Scholar 

  12. Imai H, Tatara S, Furuichi K, Oaki Y. Formation of calcium phosphate having a hierarchically laminated architecture through periodic precipitation in organic gel. Chem Commun. 2003;15:1952–3.

    Article  Google Scholar 

  13. Chang MC, Ko CC, Douglas WH. Preparation of hydroxyapatite–gelatin nanocomposite. Biomaterials. 2003;24:2853–62.

    Article  CAS  Google Scholar 

  14. Kim HW, Knowles JC, Kim HE. Porous scaffolds of gelatin–hydroxyapatite nanocomposites obtained by biomimetic approach: characterization and antibiotic drug release. J Biomed Mater Res. 2005;74(B):686–98.

    Article  Google Scholar 

  15. Tong H, Ma WT, Wang LL, Wan P, Hu JM, Cao LX. Control over the crystal phase, shape, size and aggregation of calcium carbonate via a l-aspartic acid inducing process. Biomaterials. 2004;25:3923–9.

    Article  CAS  Google Scholar 

  16. Masanori K, Hiroko NM, Takeki Y, Yoshihisa K, Kazuo T, Junzo T. Glutaradehyde cross-linked hydroxyapatite/collagen self-organized nanocomposites. Biomaterials. 2004;25:63–9.

    Article  Google Scholar 

  17. Kim HW, Kim HE, Salih V. Stimulation of osteoblast responses to biomimetic nanocomposites of gelatin–hydroxyapatite for tissue engineering scaffolds. Biomaterials. 2005;26:5221–30.

    Article  CAS  Google Scholar 

  18. Woodard JR, Hilldore AJ, Lan SK. The mechanical properties and osteoconductivity of hydroxyapatite bone scaffolds with multi-scale porosity. Biomaterials. 2007;28:45–54.

    Article  CAS  Google Scholar 

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Acknowledgments

This research was financed by National Natural Science Foundation of China (30900297 to XY Shen) and the Research Fund for the Doctoral Program of Higher Education (20090141120055 to XY Shen).

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Authors and Affiliations

  1. Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China

    Xinyu Shen, Li Chen, Xuan Cai, Hua Tong & Jiming Hu

  2. Chemical Engineering and Pharmacy School, Wuhan Institute of Technology, Wuhan, 430072, People’s Republic of China

    Tong Tong

  3. Center of Nano-Sciences and Nano-Technology Research, Wuhan University, Wuhan, 430072, People’s Republic of China

    Hua Tong

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  1. Xinyu Shen
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  2. Li Chen
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  3. Xuan Cai
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  4. Tong Tong
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  5. Hua Tong
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  6. Jiming Hu
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Correspondence to Hua Tong.

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Shen, X., Chen, L., Cai, X. et al. A novel method for the fabrication of homogeneous hydroxyapatite/collagen nanocomposite and nanocomposite scaffold with hierarchical porosity. J Mater Sci: Mater Med 22, 299–305 (2011). https://doi.org/10.1007/s10856-010-4199-x

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  • DOI: https://doi.org/10.1007/s10856-010-4199-x

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