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Bone-like nanocomposites based on self-assembled protein-based matrices with Ca2+ capturing capability

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Abstract

In the present work, bone-like nanocomposites have been successfully synthesized based on the mineralization of self-assembled protein-based microgels. Such mircogels were achieved by the in vitro reconstitution of collagen monomeric solutions in the presence of alginate in a microemulsion system. Microstructural observations revealed that the collagen-alginate composite beads possessed a nanofibrous three dimensional (3D) interconnected porous microstructure. The obtained microgels were pre-incubated in calcium-containing solution to capture Ca2+ ions, and subsequently immersed in phosphate-containing solution to initiate the formation of hydroxyapatite (HA) by an alternative incubating procedure. It was observed that a substantial amount of bone-like apatite nanocrystals were orderly and homogeneously deposited throughout the porous fibrillar networks. Herein, the collagen-alginate composite microgels served as a mineralization template for the synthesis of HA-polymer nanocomposites, which could be ideal vehicles potentially for cell carriers, bone repair and proteins and drugs delivery in tissue regeneration.

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Acknowledgements

This work is supported by the National Natural Science Foundation of China (No. 30670561, 30970729 and 50830105), the Foundation of the Ministry of Education of China (No. 20090181110067) and the National Basic Research Program of China (No. 2005CB623903 and 2007CB936102). The support from DfES of the UK/China Fellowships for Excellence Programme is gratefully acknowledged.

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Authors and Affiliations

  1. National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, People’s Republic of China

    Lin Sang, Dongmei Luo, Zhenhua Chen & Xudong Li

  2. Department of Mechanical Engineering, University College London, Torrington Place, London, WC1E 7JE, UK

    Jie Huang

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  1. Lin Sang
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  2. Jie Huang
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  3. Dongmei Luo
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  4. Zhenhua Chen
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Correspondence to Xudong Li.

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Sang, L., Huang, J., Luo, D. et al. Bone-like nanocomposites based on self-assembled protein-based matrices with Ca2+ capturing capability. J Mater Sci: Mater Med 21, 2561–2568 (2010). https://doi.org/10.1007/s10856-010-4117-2

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  • DOI: https://doi.org/10.1007/s10856-010-4117-2

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