Sensing of protein adsorption with a porous bulk composite comprising silver nanoparticles deposited on hydroxyapatite

  • Chikara Ohtsuki
  • Yuji Ichikawa
  • Hiroyuki Shibata
  • Giichiro Kawachi
  • Tsukasa Torimoto
  • Shin-ichi Ogata


Porous bulk composites were produced by depositing silver nanoparticles of diameter 11.0 ± 3.2 nm on hydroxyapatite of micrometer sizes. Adsorption of bovine serum albumin (BSA) and lysozyme (LSZ) on the composite material was observed in 2 and 10 mol m−3 phosphate buffer solutions. More BSA than LSZ was adsorbed in 2 mol m−3 phosphate buffer and this was attributed to a larger a-face surface area present in the plate- and rod-shaped hydroxyapatite compared with the c-face surface area. Peak shifts in localized surface plasmon resonance (LSPR) spectra were clearly related to adsorbed amounts of BSA and LSZ after exposure of the porous bulk composites to protein solutions. The sensing capability of the porous bulk composite results from changes in the dielectric constant of the surface fluid surrounding the silver nanoparticles. Adsorption/desorption cycles of BSA were applied to the porous bulk composite, confirming the reversibility of the sensing capability.


Hydroxyapatite Silver Nanoparticles Phosphate Buffer Solution Peak Shift Protein Adsorption 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



C. Ohtsuki appreciates the financial support received from the Daiko Foundation, Nagoya, Japan.


  1. 1.
    Link S, El-Sayed MA. Spectral properties and relaxation dynamics of surface plasmon electronic oscillations in gold and silver nanodots and nanorods. J Phys Chem B. 1999;103:8410–26.CrossRefGoogle Scholar
  2. 2.
    Kelly KL, Coronado E, Zhao LL, Schatz GC. The optical properties of metal nanoparticles: the influence of size, shape, and dielectric environment. J Phys Chem B. 2003;107:668–77.CrossRefGoogle Scholar
  3. 3.
    Haes AJ, Van Duyne RP. A nanoscale optical biosensor: sensitivity and selectivity of an approach based on the localized surface plasmon resonance spectroscopy of triangular silver nanoparticles. J Am Chem Soc. 2002;124:10596–604.CrossRefPubMedGoogle Scholar
  4. 4.
    Haes AJ, Van Duyne RP. A unified view of propagating and localized surface plasmon resonance biosensors. Anal Bioanal Chem. 2004;379:920–30.CrossRefPubMedGoogle Scholar
  5. 5.
    Gorbunoff MJ. The interaction of proteins with hydroxyapatite. Anal Biochem. 1984;136:425–45.CrossRefPubMedGoogle Scholar
  6. 6.
    Kawasaki T. Hydroxyapatite as a liquid chromatographic packing. J Chromatogr. 1991;544:147–84.CrossRefGoogle Scholar
  7. 7.
    LeGeros RZ. Properties of osteoconductive biomaterials: calcium phosphates. Clin Orthop Relat Res. 2002;395:81–98.CrossRefPubMedGoogle Scholar
  8. 8.
    Boyde A, Corsi A, Quarto R, Cancedda R, Bianco P. Osteoconduction in large macroporous hydroxyapatite ceramic implants: evidence for a complementary integration and disintegration mechanism. Bone. 1999;24:579–89.CrossRefPubMedGoogle Scholar
  9. 9.
    Ichikawa Y, Ogata S, Torimoto T, Kawachi G, Kikuta K, Ohtsuki C. Hybridization of silver nanoparticles on hydroxyapatite in an aqueous solution. J Ceram Soc Japan. 2009;117:294–8.CrossRefGoogle Scholar
  10. 10.
    Kawachi G, Watanabe T, Ogata S, Kamitakahara M, Ohtsuki C. Protein adsorption on needle-shaped hydroxyapatite prepared by hydrothermal treatment of mixture composed of CaHPO4 · 2H2O and β-Ca3(PO4)2. J Ceram Soc Japan. 2009;117:847–50.CrossRefGoogle Scholar
  11. 11.
    Ioku K, Kawachi G, Sasaki S, Fujimori H, Goto S. Hydrothermal preparation of tailored hydroxyapatite. J Mater Sci. 2006;42:1341–4.CrossRefADSGoogle Scholar
  12. 12.
    McFarland AD, Van Duvne RP. Single silver nanoparticles as real-time optical sensors with zeptomole sensitivity. Nano Lett. 2003;3:1057–62.CrossRefADSGoogle Scholar
  13. 13.
    Zhang X, Bai R, Tong YW. Selective adsorption behaviors of proteins on polypyrrole-based adsorbents. Sep Purif Technol. 2006;52:161–9.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Chikara Ohtsuki
    • 1
  • Yuji Ichikawa
    • 1
  • Hiroyuki Shibata
    • 1
  • Giichiro Kawachi
    • 1
  • Tsukasa Torimoto
    • 1
  • Shin-ichi Ogata
    • 2
  1. 1.Graduate School of EngineeringNagoya UniversityNagoyaJapan
  2. 2.Graduate School of Environment and Information ScienceYokohama National UniversityYokohamaJapan

Personalised recommendations