A novel bioactive three-dimensional β-tricalcium phosphate/chitosan scaffold for periodontal tissue engineering

  • Feng Liao
  • Yangyang Chen
  • Zubing Li
  • Yining Wang
  • Bin Shi
  • Zhongcheng Gong
  • Xiangrong Cheng


The development of suitable bioactive three-dimensional scaffold for the promotion of cellular proliferation and differentiation is critical in periodontal tissue engineering. In this study,porous β-tricalcium phosphate/chitosan composite scaffolds were prepared through a freeze-drying method. These scaffolds were evaluated by analysis of microscopic structure, porosity, and cytocompatibility. The gene expression of bone sialoprotein (BSP) and cementum attachment protein (CAP) was detected with RT-PCR after human periodontal ligament cells (HPLCs) were seeded in these scaffolds. Then cell–scaffold complexes were implanted subcutaneously into athymic mice. The protein expression of alkaline phosphatase (ALP) and osteopontin (OPN) was detected in vivo. Results indicated that composite scaffolds displayed a homogeneous three-dimensional microstructure; suitable pore size (120 μm) and high porosity (91.07%). The composite scaffold showed higher proliferation rate than the pure chitosan scaffold, and up-regulated the gene expression of BSP and CAP. In vivo, HPLCs in the composite scaffold not only proliferated but also recruited vascular tissue ingrowth. The protein expression of ALP and OPN was up-regulated in the composite scaffold. Therefore, it was suggested that the composite scaffold could promote the differentiation of HPLCs towards osteoblast and cementoblast phenotypes.


Chitosan Tissue Engineering Calcium Phosphate Alveolar Bone Periodontal Ligament 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This study was supported by Grant No. 30772445 from the Natural Science Foundation of China.


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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Feng Liao
    • 1
  • Yangyang Chen
    • 2
  • Zubing Li
    • 1
  • Yining Wang
    • 1
  • Bin Shi
    • 1
  • Zhongcheng Gong
    • 1
  • Xiangrong Cheng
    • 1
    • 3
  1. 1.Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School and Hospital of StomatologyWuhan UniversityWuhanPeople’s Republic of China
  2. 2.Department of Oncology, Tongji HospitalHuazhong University of Science and TechnologyWuhanPeople’s Republic of China
  3. 3.Department of Prosthodontics, School and Hospital of StomatologyWuhan UniversityWuhanPeople’s Republic of China

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