Two ply tubular scaffolds comprised of proteins/poliglecaprone/polycaprolactone fibers
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Electrospun bi-layer tubular hybrid scaffolds composed of poliglecaprone (PGC), polycaprolactone (PCL), elastin (E), and gelatin (G) were prepared and thereafter crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). Scanning electron microscopic (SEM) images revealed a highly porous micro-structure comprising randomly distributed non-woven fibers with the majority of fibers in submicron diameters. The EDC-crosslinking yielded an average crosslinking degree of 40%. Uni-axial tensile test of hydrated scaffolds in both longitudinal and circumferential directions revealed tensile properties, comparable to those of native arteries. The graft (PGC:PCL = 1:3) did not demonstrate significant difference before and after EDC-crosslinking in tensile strength or % strain in either longitudinal or circumferential directions. However, crosslinking increased the Young’s modulus of the graft along the longitudinal direction (from 5.84 to 8.67 MPa). On the contrary, the graft (3:1) demonstrated a significant decrease in maximum strain in both directions. Cyto-assay using human umbilical vein endothelial cells (HUVECs) showed excellent cell viability.
KeywordsUltra High Molecular Weight Polyethylene Composite Scaffold Crosslinking Degree Electrospun Scaffold Native Artery
The authors acknowledge the financial support from National Science Foundation-NSF-NIRT program under DMR-0402891. The authors are also grateful for instrumental training and support from Drs. Derrick Dean and Robin Foley of Department of Material Sciences and Engineering, and Pre-doctorals Moncy Jose of Department of Materials Science and Engineering, and Ting Feng of Department of Microbiology.
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