Skip to main content
SpringerLink
Log in

In vitro bioactivity of sol–gel-derived hydroxyapatite particulate nanofiber modified titanium

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

A chemically-etched titanium surface was modified by electrospinning a sol–gel-derived hydroxyapatite (HAp) that was subjected to calcination within the temperature range of 200–1400°C in the normative atmospheric condition. After heat treatment, crystal structures of the filmed titanium oxide and HAp on the titanium’s surface were identified using wide-angle X-ray diffraction. A highly porous layer of HAp was found to have formed on the oxidized titanium surfaces. The surfaces of three different samples; (1) electrospun HAp, (2) HAp calcined at 600°C, and (3) HAp calcined at 800°C, were investigated for their ability to foster promotion, proliferation, and differentiation of human osteoblasts (HOB) (in the 9th passage) in vitro up to 6 days. Among the three samples, cells cultured on the HAp calcined at 800°C titanium surfaces displayed the best results with regard to adhesion, growth, and proliferation of HOB. This novel method for fabrication of titanium substrates would provide a promising improvement for titanium-based medical devices over the current standards, which lack such substrates. These titanium substrates explicitly provide enhanced HOB proliferation in terms of both desired surface properties and their produced bulk quantity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  1. Hala ZC, Rolfe H. Titanium substrata composition influences osteoblastic phenotype: in vitro study. J Biomed Mater Res. 1999;47:360–6.

    Article  Google Scholar 

  2. Garcia-Alonso MC, Saldana L, Vallés G, Gonzalez-Carrasco JL, González-Cabrero J, Martínez ME, et al. In vitro corrosion behaviour and osteoblast response of thermally oxidized Ti6Al4V alloy. Biomaterials. 2003;24:19–26.

    Article  CAS  PubMed  Google Scholar 

  3. Bidez MW, Misch CE. Force transfer in implant dentistry: basic concepts and principles. J Oral Implantol. 1992;18:264–74.

    CAS  PubMed  Google Scholar 

  4. Im KH, Lee SB, Kim KM, Lee YK. Improvement of bonding strength to titanium surface by sol–gel derived hybrid coating of hydroxyapatite and titania by sol–gel process. Surf Coat Technol. 2007;202:1135–8.

    Article  CAS  Google Scholar 

  5. Michael MC. Dental implant materials: commercially pure titanium and titanium alloys. J Prosthodont. 1999;l8:40–3.

    Google Scholar 

  6. Fujibayashi S, Masashi N, Kim HM, Kokubo T, Nakamura T. Osteoinduction of porous bioactive titanium metal. Biomaterials. 2004;25:443–50.

    Article  CAS  PubMed  Google Scholar 

  7. Kasemo B, Lausmaa J. Biomaterial and implant surface: a surface science approach. Int J Oral Maxillofac Implants. 1988;3:247–59.

    CAS  PubMed  Google Scholar 

  8. Xuanyong L, Paul KC, Chuanxian D. Surface modification of titanium, titanium alloys, and related materials for biomedical applications. Mater Sci Eng R Rep. 2004;47:49–121.

    Article  Google Scholar 

  9. Okumura M, Ohgushi H, Dohi Y, Katuda T, Tamai S, Koerten HK, et al. Osteoblastic phenotype expression on the surface of hydroxyapatite ceramics. J Biomed Mater Res. 1997;37:122–9.

    Article  CAS  PubMed  Google Scholar 

  10. Zhang C, Wang JX, Feng H, Lu B, Zhang X. Repairing segmental bone defects with living porous ceramic cylinders: an experimental study in dog femora. J Biomed Mater Res. 2001;55:28–32.

    Article  CAS  Google Scholar 

  11. Li JP, Habibovica P, Doelc M, Wilsonb CE, Wijna JR, Clemens AV, et al. Bone ingrowth in porous titanium implants produced by 3D fiber deposition. Biomaterials. 2007;28:2810–20.

    Article  CAS  PubMed  Google Scholar 

  12. Kumta PN, Sfeir C, Lee DH, Olton D, Choi D. Nanostructured calcium phosphates for biomedical applications: novel synthesis and characterization. Acta Biomater. 2005;1:65–83.

    Article  PubMed  Google Scholar 

  13. Larsson C, Emanulesson L, Thomsen P, Ericson LE. Bone response to surface modified titanium implants—studies on the tissue response after 1 year to machined and electropolished implants with different oxide thicknesses. J Mater Sci Mater Med. 1997;8:721–9.

    Article  CAS  PubMed  Google Scholar 

  14. Feng B, Weng J, Yang BC, Qu SX, Zhang XD. Characterization of surface oxide films on titanium and adhesion of osteoblast. Biomaterials. 2003;24:4663–70.

    Article  CAS  PubMed  Google Scholar 

  15. Groessner-Schreiber B, Tuan RS. Enhanced extracellular matrix production an mineralization by osteoblasts cultured on titanium surfaces in vitro. J Cell Sci. 1992;101:209–17.

    CAS  PubMed  Google Scholar 

  16. Barbara DB, Thomas WH, David DD, Zvi S. Role of material surfaces in regulating bone and cartilage cell response. Biomaterials. 1996;17:137–46.

    Article  Google Scholar 

  17. Bajgai MP, Aryal S, Bhattarai SR, Remant Bahadur KC, Kim KW, Kim HY. Poly (ε-caprolactone) grafted dextran biodegradable electrospun matrix: a novel scaffold for tissue engineering. J Appl Polym Sci. 2008;108:1447–54.

    Article  CAS  Google Scholar 

  18. Aryal S, Bajgai MP, Khil MS, Kang HS, Kim HY. Biomimetic hydroxyapatite particulate nanofiber modified silicon: in vitro bioactivity. J Biomed Mater Res. 2009;88A:384–91.

    Article  CAS  Google Scholar 

  19. Hanawa T, Kamiura Y, Yamamoto S, Kohgo T, Amemiya A, Ukai H, et al. Early bone formation around calcium-ion-implanted titanium inserted into rat tibia. J Biomed Mater Res. 1997;36:131–6.

    Article  CAS  PubMed  Google Scholar 

  20. Li D, Ferguson SJ, Beutler T, Cochran DL, Sittig C, Hirt HP, et al. Biomechanical comparison of the sandblasted and acid etched and the machined and acid-etched titanium surface for dental implants. J Biomed Mater Res. 2002;60:325–32.

    Article  CAS  PubMed  Google Scholar 

  21. Frayssinet P, Tourenne F, Rouquet N, Conte P, Delga C, Bonel G. Comparative biological properties of HA plasma-sprayed coatings with different crystallinities. J Mater Sci Mater Med. 1994;5:11–7.

    Article  CAS  Google Scholar 

  22. Cheung HS, McCarty DJ. Mitogenesis induced by calcium-containing crystals: role of intracellular dissolution. Exp Cell Res. 1985;157:63–70.

    Article  CAS  PubMed  Google Scholar 

  23. Pattanayak DK, Divya P, Upadhyay S, Prasad RC, Rao BT, Rama Mohan TR. Synthesis and evaluation of hydroxyapatite ceramics. Trends Biomater Artif Organs. 2005;18:87–92.

    Google Scholar 

  24. Chen W, McCarthy TJ. Layer-by-layer deposition: a tool for polymer surface modification. Macromolecules. 1997;30:78–86.

    Article  CAS  ADS  Google Scholar 

  25. Stevens N, Priest CI, Sedev R, Ralston J. Wettability of photoresponsive titanium dioxide surfaces. Langmuir. 2003;19:3272–5.

    Article  CAS  Google Scholar 

  26. Vihola H, Laukkanen A, Valtola L, Tenhu H, Hirvonen J. Cytotoxicity of thermosensitive polymers poly(N-isopropylacrylamide), poly(N-vinylcaprolactam) and amphiphilically modified poly-(N-vinylcaprolactam). Biomaterials. 2005;26:3055–64.

    Article  CAS  PubMed  Google Scholar 

  27. Groot K, Geesink R, Klein C, Serekian P. Plasma-sprayed coatings of hydroxyl apatite. J Biomed Mater Res. 1987;21:1375–81.

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This research was supported by the Korean Research Foundation (KRF2007-211-D00032, Korean Government Project No. 10028211), Grant Funded by the Korean Government (MOEHRD), and Regional Research Centers Program of the Korean Ministry of Educational and Human Resources Development through the Center for Healthcare Technology Development, Chonbuk National University, Jeonju 562-756, Republic of Korea.

Author information

Authors and Affiliations

  1. Department of Bionanosystem Engineering, Chonbuk National University, Jeonju, 561-756, Republic of Korea

    Madhab Prasad Bajgai & Daman Chandra Parajuli

  2. Center for Healthcare Technology Development, Chonbuk National University, Jeonju, 561-756, Republic of Korea

    Soo-Jin Park

  3. Clean and science Co., Ltd, Samsung Dong, Kangnam Ku, Seoul, Republic of Korea

    Kong Hee Chu

  4. College of Veterinary Medicine, Chonbuk National University, Jeonju, 561-756, Republic of Korea

    Hyung-Sub Kang

  5. Department of Textile Engineering, Chonbuk National University, Jeonju, 561-756, Republic of Korea

    Kong Hee Chu & Hak Yong Kim

Authors
  1. Madhab Prasad Bajgai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Daman Chandra Parajuli
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Soo-Jin Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kong Hee Chu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hyung-Sub Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hak Yong Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hak Yong Kim.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bajgai, M.P., Parajuli, D.C., Park, SJ. et al. In vitro bioactivity of sol–gel-derived hydroxyapatite particulate nanofiber modified titanium. J Mater Sci: Mater Med 21, 685–694 (2010). https://doi.org/10.1007/s10856-009-3902-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-009-3902-2

Keywords

Search

Navigation