Cartilage replacement by use of hybrid systems of autologous cells and polyethylene: an experimental study
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This study used porous polyethylene (PE) as a scaffold in an animal model system. The surface of the scaffolds was either modified with collagen II coating or first functionalized by oxygen plasma treatment and then coated with collagen II. The specimens were inoculated with autologous chondrocytes and transplanted into the concha of guinea pigs. Bare scaffolds were used as controls. Periods of 1, 6, and 12 months after implantation, samples of cells containing specimens and control samples were evaluated microscopically. As a result, the pre-seeded specimens were better integrated into the surrounding tissue than cell-free PE-specimens. Also a weaker immune reaction and an improved cartilage generation could be detected in the pre-seeded specimen. Compared to the other surface modifications, no further improvement of cartilage development was observed in the long term in vivo animal experimental study.
KeywordsSurface Modification Oxygen Plasma Cell Seeding Collagen Coating Autologous Cell
The authors thank Dr. Jürgen Vogel from the Department of Bioengineering of the MLU Halle-Wittenberg for performing the surface modification of the PE materials. This project has been funded by ministry of education of Sachsen-Anhalt Grant No. 3308A/0080B.
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