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Investigation of polymeric amphiphilic nanoparticles as antitumor drug carriers

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Abstract

In this paper, polymeric amphiphilic nanoparticles based on oleoyl–chitosan (OCH) with different degrees of substitution (DS, 5%, 11% and 27%) were prepared by Oil/Water emulsification method. Mean diameters of the nanoparticles were 327.4 nm, 255.3 nm and 192.6 nm, respectively. Doxorubicin (DOX) was efficiently loaded into OCH nanoparticles and provided a sustained released after a burst release in PBS. These nanoparticles showed no cytotoxicity to mouse embryo fibroblasts (MEF) and low hemolysis rates (<5%). The results of SDS-PAGE indicated that bovine calf serum (BCS) adsorption on OCH nanoparticles was inhibited by smaller particle size. Cellular uptake was evaluated by incubating fluorescence labeled OCH nanoparticles with human lung carcinoma cells (A549) and mouse macrophages (RAW264.7). Cellular uptake of OCH nanoparticles was time––and concentration––dependent. Finding the appropriate incubation time and concentration of OCH nanoparticles used as drug carriers might decrease phagocytic uptake, increase cancer cell uptake and ultimately improve therapeutic efficiency of antitumor therapeutic agents.

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Acknowledgements

This work was supported by grants from national natural science foundation of China (NSFC, 30770582) and international science and technology cooperation project (ISTCP, 2008DFA31640).

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Authors and Affiliations

  1. College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao, 266003, People’s Republic of China

    Jing Zhang, Xi Guang Chen & Cheng Sheng Liu

  2. The Graduate School of Biotechnology, Korea University, Seoul, 136-701, South Korea

    Hyun Jin Park

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  1. Jing Zhang
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Correspondence to Xi Guang Chen.

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Zhang, J., Chen, X.G., Liu, C.S. et al. Investigation of polymeric amphiphilic nanoparticles as antitumor drug carriers. J Mater Sci: Mater Med 20, 991–999 (2009). https://doi.org/10.1007/s10856-008-3656-2

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