Calcium–phosphate–silicate composite bone cement: self-setting properties and in vitro bioactivity
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In this study, a novel low temperature setting calcium phosphate–silicate cement was obtained by mixing CaHPO4 · 2H2O (DCPD) and Ca3SiO5 (C3S) with 0.75 M sodium phosphate buffers (pH = 7.0) as liquid phase. The self-setting properties of the obtained DCPD/C3S paste with liquid to powder ratio (L/P) of 0.6 ml/g, such as setting times, injectability, degradability and compressive strength were investigated and compared with that of DCPD/CaO cement system. The results indicated that, with the weight ratio of C3S varied from 20% to 40%, the workable DCPD/C3S pastes could set within 20 min, and the hydrated cement showed significantly higher compressive strength (around 34.0 MPa after 24 h) than that of the DCPD/CaO cement system (approximately 10.0 MPa). Furthermore, the in vitro pH value of the cements was investigated by soaking in simulated body fluid (SBF) for 12 h, and the result indicated that the DCPD/C3S did not induce significant increase or decrease of pH value in SBF. Additionally, the composite cement possesses better ability to support and stimulate cell proliferation than the DCPD/CaO cement. With good hydraulic properties, improved biocompatibility and moderate degradability, the novel DCPD/C3S bone cement may be a potential candidate as bone substitute.
KeywordsApatite Simulated Body Fluid Calcium Silicate Hydrate Composite Cement Calcium Phosphate Cement
This work is supported by the National Basic Science Research Program of China (973 Program) (Grant No.: 2005CB522704), and the funds of the Chinese Academy of Sciences for Key Topics in Innovation Engineering (Grant No.: KGCX2-YW-207) and Shanghai Institute of Ceramics, Chinese Academy of Sciences (Grant No.: SCX0606).
- 4.W.E. Brown, L.C. Chow, US Patent No. 4,518,430 (1985)Google Scholar
- 6.W.E. Brown, L.C. Chow, in Cement Research Progress, ed. by P.W. Brown (American Ceramic Society, Westerville, OH, 1986), pp. 352–379Google Scholar
- 19.ISO/EN 10993-5:1999. Biological evaluation of medical devices-Part 5. Tests for cytotoxicity, in vitro methods: 8.2 tests on extractGoogle Scholar
- 22.T. Kokubo, M. Kushiyani, Y. Ebisawa, T. Kitsugi, S.K. Kotani, K. Oura, in Bioceramics, ed. by K. Aoki, K. Sawai (Ishiyaku EuroAmerica, Tokyo, 1989), pp. 157–162Google Scholar
- 32.M.A. Harrison, I.F. Rae, General Techniques for Cell Culture (Cambridge University Press, Cambridge, 1997), p. 7Google Scholar
- 34.L.L. Hench, J.K. West, Life Chem. Rep. 13, 187 (1996)Google Scholar