In vitro gentamicin sustained and controlled release from chitosan cross-linked films
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A novel wound dressing film was investigated for controlled and sustained delivery of gentamicin, while covering and protecting the wound. Chitosan was cross-linked with Hexamethylene 1,6-bis (aminocarboxysulfonate) to prepare the wound dressing films. Cross-linking reaction was required to control the water retention and release of encapsulated gentamicin. Cross-linked films swell less and became more hydrophilic than chitosan film itself. However, this behavior was inversely proportional to the degree of cross-linking. In vitro gentamicin release from the cross-linked films, at physiological conditions of pH and temperature, was studied for 2 weeks. The effects of gentamicin initial concentration and cross-linking ratio on the kinetics of gentamicin release were evaluated. Results showed that the diffusion rate was governed by initial concentration of gentamicin and degree of cross-linking, since higher gentamicin initial concentration and degree of cross-linking promoted the slower release, while lower gentamicin initial concentration and degree of cross-linking promoted the faster one.
KeywordsChitosan Contact Angle Gentamicin Chitosan Film Hexamethylene
The authors are thankful to the department of Restorative Dentistry, University of Texas Health Science Center at San Antonio, TX, USA and the department of Polymer Technology, School of Chemical Engineering, State University of Campinas, SP, Brazil for providing necessary facilities for this work. The authors also thank CNPq and CAPES for the financial support.
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