Interactions between human neutrophils and mucin-coated surfaces

  • Tomas Sandberg
  • Jan Carlsson
  • Marjam Karlsson Ott


Recently, we showed microscopically that bovine (BSM), porcine (PGM) and human (MG1) mucin coatings could suppress the adhesion of neutrophils to a polyethylene terephthalate-based model biomaterial (Thermanox). Here, using the release of reactive oxygen species (ROS) as a marker of material-induced neutrophil activation, the strong surface-passivating effects of these mucin coatings were corroborated. Under optimal adsorption conditions, all mucin species performed equally well, thus indicating a high degree of functional homology between the mucins. Cell adhesion and morphology correlated well with the release of ROS. Quartz crystal microbalance (QCM-D) analysis linked low neutrophil activation to efficient mucin surface-shielding. Interestingly, the shielding power appeared equal for thick expanded and thin compact mucin coatings. Combined mucin-serum coatings were found to be highly surface-passivating. Particularly, since our data suggested partly synergistic mucin-serum action, we highlight the possibility that pre-adsorbed mucins could provide favorable support for adsorbing host components.


Human Serum Albumin Composite Coating Neutrophil Activation Quartz Crystal Microbalance With Dissipation Total Ionic Strength 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We greatly acknowledge Prof. Karin Caldwell (Department of Physical and Analytical Chemistry, Uppsala University) for introducing us to the field and providing us with valuable comments during the manuscript preparation. We thank Lena Gröndahl and Riitta Laukkanen-Mållberg (Department of Clinical Chemistry and Pharmacology, Uppsala University) for help with the neutrophil isolation. Leif Ljungh (Department of Medical Cell Biology, Uppsala University) and Dr. Jonas Andersson (Department of Clinical Immunology, Uppsala University) are acknowledged for help with the SEM analysis and the human serum preparation, respectively. Finally, Prof. Per Venge (Department of Clinical Chemistry and Pharmacology, Uppsala University) is acknowledged with gratitude valuable discussions regarding the neutrophil biology.


  1. 1.
    J.M. Anderson, in Biomaterials Science, ed. by B.D. Ratner, A.S. Hoffman, F.J. Schoen, J.E. Lemons (Elsevier, Oxford, 2004), p. 296Google Scholar
  2. 2.
    A.S. Hoffman, J.A. Hubbell, in Biomaterials Science, ed. by B.D. Ratner, A.S. Hoffman, F.J. Schoen, J.E. Lemons (Elsevier, Oxford, 2004), p. 225Google Scholar
  3. 3.
    J. Perez-Vilar, in Encyclopedia of Biological Chemistry, ed. by W.J. Lennarz, M.D. Lane (Elsevier, Oxford, 2004), p. 758Google Scholar
  4. 4.
    B.J.W. Klinken, J. Dekker, H.A. Büller, A.W.C. Einerhand, Am. J. Physiol. 269, G613 (1995)PubMedGoogle Scholar
  5. 5.
    R. Bansil, B.S. Turner, Curr. Opin. Colloid Interface Sci. 11, 164 (2006). doi: 10.1016/j.cocis.2005.11.001 CrossRefGoogle Scholar
  6. 6.
    M.A. Hollingsworth, B.J. Swanson, Nat. Rev. Cancer 4, 45 (2004). doi: 10.1038/nrc1251 PubMedCrossRefGoogle Scholar
  7. 7.
    L. Shi, Trends Glycosci. Glycotechnol. 12, 229 (2000)Google Scholar
  8. 8.
    L. Shi, R. Ardehali, K.D. Caldwell, P. Valint, Colloids Surf. B 17, 229 (2000). doi: 10.1016/S0927-7765(99)00121-6 CrossRefGoogle Scholar
  9. 9.
    T. Sandberg, H. Blom, K.D. Caldwell, J. Biomed. Mater. Res. A (in press). doi: 10.1002/jbm.a.32266
  10. 10.
    R.N. Mitchell, in Biomaterials Science, ed. by B.D. Ratner, A.S. Hoffman, F.J. Schoen, J.E. Lemons (Elsevier, Oxford, 2004), p. 304Google Scholar
  11. 11.
    H. Nygren, M. Broberg, C. Eriksson, H. Sahlin, N. Yahyapour, Colloids Surf. B 22, 87 (2001). doi: 10.1016/S0927-7765(00)00216-2 CrossRefGoogle Scholar
  12. 12.
    A. Prakobphol, K. Tangemann, S.D. Rosen, C.I. Hoover, H. Leffler, S.J. Fisher, Biochemistry 38, 6817 (1999). doi: 10.1021/bi990145m PubMedCrossRefGoogle Scholar
  13. 13.
    B.M. Fischer, J.A. Voynow, Am. J. Respir. Cell Mol. Biol. 26, 447 (2002)PubMedGoogle Scholar
  14. 14.
    K.P. O’Boyle, T. Chen, S. Kozlowski, Scand. J. Immunol. 52, 46 (2000). doi: 10.1046/j.1365-3083.2000.00756.x PubMedCrossRefGoogle Scholar
  15. 15.
    M.L.R. Aknin, M. Berry, A.D. Dick, D. Khan-Lim, Cell Tissue Res. 318, 545 (2004). doi: 10.1007/s00441-004-0957-8 PubMedCrossRefGoogle Scholar
  16. 16.
    T. Sandberg, J. Carlsson, M. Karlsson Ott, Microsc. Res. Tech. 70, 864 (2007). doi: 10.1002/jemt.20489 PubMedCrossRefGoogle Scholar
  17. 17.
    S.L. Cooper, S.A. Visser, R.W. Hergenrother, N.M.K Lamba, in Biomaterials Science, ed. by B.D. Ratner, A.S. Hoffman, F.J. Schoen, J.E. Lemons (Elsevier, Oxford, 2004), p. 67Google Scholar
  18. 18.
    P.K. Smith, R.I. Krohn, G.T. Hermanson, A.K. Mallia, F.H. Gartner, M.D. Provenzano et al., Anal. Biochem. 150, 76 (1985). doi: 10.1016/0003-2697(85)90442-7 PubMedCrossRefGoogle Scholar
  19. 19.
    B.T. Doumas, Clin. Chem. 21, 1159 (1975)PubMedGoogle Scholar
  20. 20.
    T. Sandberg, L. Mellin, U. Gelius, K.D. Caldwell, J. Colloid Interface Sci. (Submitted)Google Scholar
  21. 21.
    L. Håkansson, P. Venge, Scand. J. Immunol. 11, 271 (1980). doi: 10.1111/j.1365-3083.1980.tb00235.x PubMedCrossRefGoogle Scholar
  22. 22.
    C. Dahlgren, A. Karlsson, J. Immunol. Methods 232, 3 (1999). doi: 10.1016/S0022-1759(99)00146-5 PubMedCrossRefGoogle Scholar
  23. 23.
    F. Höök, M. Rodahl, P. Brzezinski, B. Kasemo, Langmuir 14, 729 (1998). doi: 10.1021/la970815u CrossRefGoogle Scholar
  24. 24.
    M.V. Voinova, M. Rodahl, M. Jonsson, B. Kasemo, Phys. Scr. 59, 391 (1999). doi: 10.1238/Physica.Regular.059a00391 CrossRefADSGoogle Scholar
  25. 25.
    J. Tsai, R. Taylor, C. Chothia, M. Gerstein, J. Mol. Biol. 290, 253 (1999). doi: 10.1006/jmbi.1999.2829 PubMedCrossRefGoogle Scholar
  26. 26.
    T. Tammelin, J. Merta, L.-S. Johansson, P. Stenius, Langmuir 20, 10900 (2004). doi: 10.1021/la0487693 PubMedCrossRefGoogle Scholar
  27. 27.
    X. Cao, R. Bansil, K.R. Bhaskar, B.S. Turner, J.T. Lamont, N. Niu et al., Biophys. J. 76, 1250 (1999)PubMedCrossRefGoogle Scholar
  28. 28.
    S. Lee, M. Muller, R. Kurosch, N.D. Spencer, Langmuir 21, 8344 (2005). doi: 10.1021/la050779w PubMedCrossRefGoogle Scholar
  29. 29.
    L.E. Bromberg, D.P. Barr, Biomacromolecules 1, 325 (2000). doi: 10.1021/bm005532m PubMedCrossRefGoogle Scholar
  30. 30.
    Z. Hong, B. Chasan, R. Bansil, B.S. Turner, K.R. Bhaskar, N.H. Afdhal, Biomacromolecules 6, 3458 (2005). doi: 10.1021/bm0505843 PubMedCrossRefGoogle Scholar
  31. 31.
    D.J. Thornton, K. Rousseau, M.A. McGuckin, Annu. Rev. Physiol. 70, 459 (2008). doi: 10.1146/annurev.physiol.70.113006.100702 PubMedCrossRefGoogle Scholar
  32. 32.
    C.L. Hattrup, S.J. Gendler, Annu. Rev. Physiol. 70, 431 (2008). doi: 10.1146/annurev.physiol.70.113006.100659 PubMedCrossRefGoogle Scholar
  33. 33.
    A.A. Feiler, A. Sahlholm, T. Sandberg, K.D. Caldwell, J. Colloid Interface Sci. 315, 475 (2007). doi: 10.1016/j.jcis.2007.07.029 PubMedCrossRefGoogle Scholar
  34. 34.
    J.R. Keogh, F.F. Velander, J.W. Eaton, J. Biomed. Mater. Res. 26, 441 (1992). doi: 10.1002/jbm.820260403 PubMedCrossRefGoogle Scholar
  35. 35.
    J.M. Kao, R. Rose, M. Yousef, S.K. Hunter, V.G.J. Rodgers, J. Biomed. Mater. Res. 47, 537 (1999). doi:10.1002/(SICI)1097-4636(19991215)47:4<537::AID-JBM10>3.0.CO;2-IPubMedCrossRefGoogle Scholar
  36. 36.
    L. Lindh, I.E. Svendsen, O. Svensson, M. Cardenas, T. Arnebrant, J. Colloid Interface Sci. 310, 74 (2007). doi: 10.1016/j.jcis.2007.01.086 PubMedCrossRefGoogle Scholar
  37. 37.
    S.R. Hanson, in Biomaterials Science, ed. by B.D. Ratner, A.S. Hoffman, F.J. Schoen, J.E. Lemons (Elsevier, Oxford, 2004), p. 332Google Scholar
  38. 38.
    R.J Johnson, in Biomaterials Science, ed. by B.D. Ratner, A.S. Hoffman, F.J. Schoen, J.E. Lemons (Elsevier, Oxford, 2004). p. 318Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Tomas Sandberg
    • 1
  • Jan Carlsson
    • 1
  • Marjam Karlsson Ott
    • 1
  1. 1.Department of Physical and Analytical Chemistry, Division of Surface Biotechnology, BMCUppsala UniversityUppsalaSweden

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