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In vitro evaluation of freeze-dried bone allografts combined with platelet rich plasma and human bone marrow stromal cells for tissue engineering

  • Elisabetta Cenni
  • Francesca Perut
  • Gabriela Ciapetti
  • Lucia Savarino
  • Dante Dallari
  • Annarita Cenacchi
  • Cesare Stagni
  • Armando Giunti
  • Pier Maria Fornasari
  • Nicola Baldini
Article

Abstract

Freeze-dried bone allograft (FDBA) might be more effective in combination with platelet rich plasma (PRP) and bone marrow stromal cells (BMSC) in accelerating bone healing. The isolation of BMSC through density gradient (pBMSC) is not extensively applicable in clinical practice, because it increases the risk of infection. Alternatively, BMSC can be concentrated by simple centrifugation (wBMSC) directly in the operating room. However, we do not know if wBMSC act in the same way as pBMSC. BMSC from 10 donors were tested whether, in the presence of a combination of FDBA and autologous PRP, the osteogenic differentiation of the cells concentrated by simple centrifugation (wBMSC + FDBA + PRP) was similar to that of pBMSC. Cell-associated alkaline phosphatase, osterix and fibroblast growth factor-2 were higher in wBMSC + FDBA + PRP. In conclusion, the combination of FDBA and PRP had a favouring effect on the differentiation towards osteoblasts and allowed BMSC concentrated by simple centrifugation to differentiate as fast as BMSC purified by density gradient.

Keywords

Osteogenic Differentiation Platelet Rich Plasma Bone Marrow Stromal Cell High Tibial Osteotomy Calcium Gluconate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We thank Istituto Ortopedico Rizzoli, ‘Ricerca corrente’, the “Ministero dell’Istruzione, dell’Università e della Ricerca” of Italy, “Fondo per gli Investimenti della Ricerca di Base” (project n° RBAU01N79B_001), the “Fondazione Monte dei Paschi” of Siena (Italy).

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Elisabetta Cenni
    • 1
  • Francesca Perut
    • 1
  • Gabriela Ciapetti
    • 1
  • Lucia Savarino
    • 1
  • Dante Dallari
    • 2
  • Annarita Cenacchi
    • 3
  • Cesare Stagni
    • 2
  • Armando Giunti
    • 1
    • 2
  • Pier Maria Fornasari
    • 3
  • Nicola Baldini
    • 1
    • 2
  1. 1.Laboratory for Pathophysiology of Orthopaedic ImplantsIstituto Ortopedico RizzoliBolognaItaly
  2. 2.Department of Orthopaedic SurgeryIstituto Ortopedico RizzoliBolognaItaly
  3. 3.Unit of Immunohematology and Transfusion MedicineIstituto Ortopedico RizzoliBolognaItaly

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