Skip to main content

Advertisement

SpringerLink
Log in

Preparation of hydroxyapatite spheres with an internal cavity as a scaffold for hard tissue regeneration

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Microparticulates are currently regarded as a useful matrix for the delivery of bioactive molecules and tissue cells. Herein, hydroxyapatite (HA) spherical microparticulates with an internal cavity were produced using an oil-in-water emulsion technique. The HA slurry in the organic solvent was formulated into spherical particles in a water bath containing a surfactant. Rapid evaporation of the solvent helped create a cavity within the microparticulates. The microparticulates were heat-treated at 1,200°C to produce bioactive HA particles with a mean size of approximately 363 μm. Osteoblastic cells were observed to spread and grow favorably over the surface as well as within the cavity of the microparticulates. The currently developed HA microparticulates with an internal cavity are considered to be useful as a scaffolding matrix for bone tissue engineering and direct filling skeletal defects.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. S. Freiberg, X.X. Zhu, Int. J. Pharm. 282, 1 (2004)

    Article  CAS  Google Scholar 

  2. K.J. Pekarek, J.S. Jacob, E. Mathiowitz, Nature 367, 258 (1994)

    Article  CAS  Google Scholar 

  3. M. Borden, M. Attawia, Y. Khan, C.T. Laurencin, Biomaterials 23, 551 (2002)

    Article  CAS  Google Scholar 

  4. H.J. Gu, H.H. Lee, H.W. Kim, Tissue Eng. 13, 965 (2007)

    Article  Google Scholar 

  5. Q.Q. Qiu, P. Ducheyne, P.S. Ayyaswamy, J. Biomed. Mater. Res. 52, 66 (2000)

    Article  CAS  Google Scholar 

  6. A.M. Rokstad, S. Holtan, B. Strand, B. Steinkjer, L. Ryan, B. Kulseng, G. Skjak-Braek, T. Espevik, Cell Transplant. 11, 313 (2002)

    Google Scholar 

  7. D. Kato, M. Takeuchi, T. Sakurai, S. Furukawa, H. Mizokami, M. Sakata, C. Hirayama, M. Kunitake, Biomaterials 24, 4253 (2003)

    Article  CAS  Google Scholar 

  8. H. Maeda, T. Kasuga, Acta Biomater. 2, 403 (2006)

    Article  Google Scholar 

  9. H.W. Kim, B.H. Yoon, H.E. Kim, J. Mater. Sci. Mater. Med. 16, 1105 (2005)

    Article  CAS  Google Scholar 

  10. Q.Q. Qiu, P. Ducheyne, P.S. Ayyaswamy, Biomaterials 20, 989 (1999)

    Article  CAS  Google Scholar 

  11. P.W. Brown, B. Constantz, Hydroxyapatite and Related Materials (CRC press, Boca Raton, 1994)

    Google Scholar 

  12. W. Paul, C.P. Sharma, J. Biomater. Appl. 17, 253 (2003)

    Article  CAS  Google Scholar 

  13. K.A. Al Ruhaimi, Int. J. Oral. Maxillofac Implants. 16, 105 (2001)

    CAS  Google Scholar 

  14. P.C. Hobar, M. Pantaloni, H.S. Byrd, Clin. Plast. Surg. 27, 557 (2000)

    CAS  Google Scholar 

  15. J.M. Schmitt, D.C. Buck, S.P. Joh, S.E. Lynch, J.O. Hollinger, J. Periodontol. 68, 1043 (1997)

    CAS  Google Scholar 

  16. H. Yoshikawa, A. Myoui, J. Artif. Organs. 8, 131 (2005)

    Article  CAS  Google Scholar 

  17. Y. Behairy, M. Jasty, Orthop. Clin. North Am. 30, 661 (1999)

    Article  CAS  Google Scholar 

  18. C.J. Bae, H.W. Kim, Y.H. Koh, H.E. Kim, J. Mater. Sci. Mater. Med. 17, 517 (2006)

    Article  CAS  Google Scholar 

  19. B.G. Santoni, G.E. Pluhar, T. Motta, D.L. Wheeler, Biomed. Mater. Eng. 17, 277 (2007)

    CAS  Google Scholar 

  20. H.W. Kim, J.C. Knowles, H.E. Kim, J. Mater. Sci. Mater. Med. 16, 189 (2005)

    Article  Google Scholar 

  21. I. Ono, T. Yamashita, H.Y. Jin, Y. Ito, H. Hamada, Y. Akasaka, M. Nakasu, T. Ogawa, K. Jimbow, Biomaterials 25, 4709 (2004)

    Article  CAS  Google Scholar 

  22. H.W. Kim, H.E. Kim, J.C. Knowles, Biomaterials 25, 1279 (2004)

    Article  CAS  Google Scholar 

  23. M.P. Ferraz, A.Y. Mateus, J.C. Sousa, F.J. Monteiro, J. Biomed. Mater. Res. A 81, 994 (2007)

    CAS  Google Scholar 

  24. S.P. Victor, T.S. Kumar, J. Mater. Sci. Mater. Med. 19, 283 (2008)

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the Korea Science and Engineering Foundation (KOSEF) grants funded by the Korea government (MOST) (No. R01-2007-000-20183-0 and M10646020001-06N4602-00110).

Author information

Authors and Affiliations

  1. Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan, 330-714, South Korea

    Hae-Hyoung Lee, Seok-Jung Hong & Hae-Won Kim

  2. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, 330-714, South Korea

    Hae-Hyoung Lee & Hae-Won Kim

  3. Department of Biochemistry, College of Medicine, Inha University, Incheon, South Korea

    Jun-Hyeog Jang

  4. Department of Oral and Maxillofacial Surgery, School of Dentistry, Dankook University, Cheonan, 330-714, South Korea

    Chul-Hwan Kim

  5. Department of Oral and Maxillofacial Pathology, School of Dentistry, Wonkwang Unversity, Iksan, 570-749, South Korea

    Eun-Cheol Kim

  6. Department of Oral Pathology & Institute for Hard tissue and Bio-tooth Regeneration, School of Dentistry, Kyungpook National University, Daegu, 700-422, South Korea

    Hong-In Shin

Authors
  1. Hae-Hyoung Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Seok-Jung Hong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chul-Hwan Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Eun-Cheol Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jun-Hyeog Jang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hong-In Shin
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Hae-Won Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hae-Won Kim.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, HH., Hong, SJ., Kim, CH. et al. Preparation of hydroxyapatite spheres with an internal cavity as a scaffold for hard tissue regeneration. J Mater Sci: Mater Med 19, 3029–3034 (2008). https://doi.org/10.1007/s10856-008-3435-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-008-3435-0

Keywords

Search

Navigation