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Evaluation of physical–chemical properties and biocompatibility of a microrough and smooth bioactive glass particles

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Abstract

The purpose of this study was to evaluate physical–chemical and biocompatibility characteristics of a simple synthesis and low cost experimental bioactive glass. Physical and chemical properties were analyzed using scanning electron microscopy (SEM), X-ray energy dispersive (EDX), X-ray fluorescence (XRF) and X-ray diffraction (XRD). The biomaterials were subcutaneously implanted into rats, according to the following groups: G1, PerioGlas™; G2, Biogran™, G3, Experimental Bioactive Glass U (BGU) and G4, Control (Sham). After 7, 15, 21, 45, and 60 days, 5 animals/group/period were sacrificed and the subcutaneous tissue was dissected for histological and histometric analysis, considering inflammatory reaction and granulation area, presence of polymorphonuclear (PMN), monuclear (MN) and fibroblast (F) cells. SEM analysis of biomaterials showed irregular particles with different surface characteristics. EDX showed calcium, oxygen, sodium, phosphorus and silicon; XRF revealed silica oxide (SiO2), sodium oxide (Na2O), calcium oxide (CaO) and phosphorus oxide (P2O5). XRD indicated non crystalline phase. Measurement of tissue reaction showed similar results among the experimental groups at 45 and 60 days. No difference was found for PMN, MN and F cell counts. All biomaterials exhibited partial resorption. In conclusion, the experimental bioactive glass analyzed showed physical and chemical characteristics similar to the commercially available biomaterials, and was considered biocompatible, being partially reabsorbed in the subcutaneous tissue.

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Acknowledgements

This work was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil.

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Authors and Affiliations

  1. Department of Dentistry, State University of Ponta Grossa, CEP-84030-900, Uvaranas, Ponta Grossa, PR, Brazil

    Ariadne Cristiane Cabral da Cruz, Márcia Thaís Pochapski, Gibson Luiz Pilatti & Fábio André Santos

  2. Department of Histology, Federal University of Santa Catarina, Florianópolis, SC, Brazil

    Ricardo Tramonti

  3. Department of Chemistry, State University of Ponta Grossa, Ponta Grossa, PR, Brazil

    José Caetano Zurita da Silva & Augusto Celso Antunes

Authors
  1. Ariadne Cristiane Cabral da Cruz
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  2. Márcia Thaís Pochapski
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  3. Ricardo Tramonti
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  4. José Caetano Zurita da Silva
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  5. Augusto Celso Antunes
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  6. Gibson Luiz Pilatti
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  7. Fábio André Santos
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Correspondence to Fábio André Santos.

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da Cruz, A.C.C., Pochapski, M.T., Tramonti, R. et al. Evaluation of physical–chemical properties and biocompatibility of a microrough and smooth bioactive glass particles. J Mater Sci: Mater Med 19, 2809–2817 (2008). https://doi.org/10.1007/s10856-008-3407-4

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  • DOI: https://doi.org/10.1007/s10856-008-3407-4

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