Evaluation of physical–chemical properties and biocompatibility of a microrough and smooth bioactive glass particles

  • Ariadne Cristiane Cabral da Cruz
  • Márcia Thaís Pochapski
  • Ricardo Tramonti
  • José Caetano Zurita da Silva
  • Augusto Celso Antunes
  • Gibson Luiz Pilatti
  • Fábio André Santos


The purpose of this study was to evaluate physical–chemical and biocompatibility characteristics of a simple synthesis and low cost experimental bioactive glass. Physical and chemical properties were analyzed using scanning electron microscopy (SEM), X-ray energy dispersive (EDX), X-ray fluorescence (XRF) and X-ray diffraction (XRD). The biomaterials were subcutaneously implanted into rats, according to the following groups: G1, PerioGlas™; G2, Biogran™, G3, Experimental Bioactive Glass U (BGU) and G4, Control (Sham). After 7, 15, 21, 45, and 60 days, 5 animals/group/period were sacrificed and the subcutaneous tissue was dissected for histological and histometric analysis, considering inflammatory reaction and granulation area, presence of polymorphonuclear (PMN), monuclear (MN) and fibroblast (F) cells. SEM analysis of biomaterials showed irregular particles with different surface characteristics. EDX showed calcium, oxygen, sodium, phosphorus and silicon; XRF revealed silica oxide (SiO2), sodium oxide (Na2O), calcium oxide (CaO) and phosphorus oxide (P2O5). XRD indicated non crystalline phase. Measurement of tissue reaction showed similar results among the experimental groups at 45 and 60 days. No difference was found for PMN, MN and F cell counts. All biomaterials exhibited partial resorption. In conclusion, the experimental bioactive glass analyzed showed physical and chemical characteristics similar to the commercially available biomaterials, and was considered biocompatible, being partially reabsorbed in the subcutaneous tissue.


Bioactive Glass Tissue Reaction Acid Etching Calcium Oxide Autogenous Bone Graft 



This work was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil.


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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Ariadne Cristiane Cabral da Cruz
    • 1
  • Márcia Thaís Pochapski
    • 1
  • Ricardo Tramonti
    • 2
  • José Caetano Zurita da Silva
    • 3
  • Augusto Celso Antunes
    • 3
  • Gibson Luiz Pilatti
    • 1
  • Fábio André Santos
    • 1
  1. 1.Department of DentistryState University of Ponta GrossaUvaranas, Ponta GrossaBrazil
  2. 2.Department of HistologyFederal University of Santa CatarinaFlorianópolisBrazil
  3. 3.Department of ChemistryState University of Ponta GrossaPonta GrossaBrazil

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