A PLA/calcium phosphate degradable composite material for bone tissue engineering: an in vitro study
- 372 Downloads
Biodegradable polymers reinforced with an inorganic phase such as calcium phosphate glasses may be a promising approach to fulfil the challenging requirements presented by 3D porous scaffolds for tissue engineering. Scaffolds’ success depends mainly on their biological behaviour. This work is aimed to the in vitro study of polylactic acid (PLA)/CaP glass 3D porous constructs for bone regeneration. The scaffolds were elaborated using two different techniques, namely solvent-casting and phase-separation. The effect of scaffolds’ micro and macrostructure on the biological response of these scaffolds was assayed. Cell proliferation, differentiation and morphology within the scaffolds were studied. Furthermore, polymer/glass scaffolds were seeded under dynamic conditions in a custom-made perfusion bioreactor. Results indicate that the final architecture of the solvent-cast or phase separated scaffolds have a significant effect on cells’ behaviour. Solvent-cast scaffolds seem to be the best candidates for bone tissue engineering. Besides, dynamic seeding yielded a higher seeding efficiency in comparison with the static method.
KeywordsMG63 Cell Bone Tissue Engineering Glass Particle Solvent Cast Static Seeding
Financial funding from the Spanish Science Ministry is acknowledged (MAT2005-07244).
- 2.H.L. Holtorf, J.A. Jansen, A.G. Mikos, Adv. Exp. Med. Biol. 585, 225 (2006)Google Scholar
- 3.R.I. Abousleiman, V.I. Sikavitsas, Adv. Exp. Med. Biol. 585, 243 (2006)Google Scholar
- 10.J.E. Davies, J.M. Karp, D. Baksh. Methods of Tissue Engineering (Academic Press, San Diego,2002), p. 333Google Scholar
- 11.J. Handschel, H.P. Wiesmann, R. Depprich, N.R. Kubler, U. Meyer, Int. J. Oral Maxillofac. Implants 21, 890 (2006)Google Scholar
- 31.F.J. O’Briena, E. Farrell, M.A. Waller et al., in Topics in bio-mechanical engineering, ed. by P.J. Prendergast, P.E. McHugh (Trinity Centre for Bioengineering & National Centre for Biomedical Engineering Science, 2004), p. 172Google Scholar
- 37.K.-W. Lee, S. Wang, L. Lu et al., Tissue Engineering 12 (2006)Google Scholar
- 39.M. Navarro, E. Engel, I. Amaral, M. Barbosa, J.A. Planell, M.P. Ginebra, J. Biomed. Mater. Res. doi: 10.1002/jbm.a.31546
- 40.L. Montanaro, C.R. Arciola, D. Campoccia, M. Cervellati, Biomaterials 23, (2002)Google Scholar
- 41.J. Yao, S. Radin, G. Reilly, P.S. Leboy, P. Ducheyne, J. Biomed. Mater. Res. A 75, 794 (2005)Google Scholar
- 44.A. El Ghannam, H. Amin, T. Nasr, A. Shama, Int. J. Oral. Maxillofac. Implants. 19, 184 (2004)Google Scholar
- 45.Z. Schwartz, C.H. Lohmann, J. Oefinger, L.F. Bonewald, D.D. Dean, B.D. Boyan, Adv. Dent. Res. 13, 38 (1999)Google Scholar