Skip to main content
SpringerLink
Log in

Surface energy of hydroxyapatite and β-tricalcium phosphate ceramics driving serum protein adsorption and osteoblast adhesion

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

The main objective of this work was to evaluate the specific role of calcium phosphates surface energy on serum protein adsorption and human osteoblast adhesion, by isolating chemical effects from those caused by topography. Highly dense phosphate ceramics (single-phase hydroxyapatite HA and β-tricalcium phosphates β-TCP) presenting two distinct nano roughnesses were produced. Some samples were gold-sputter coated in order to conveniently mask the surface chemical effects (without modification of the original roughness) and to study the isolated effect of surface topography on cellular behavior. The results indicated that the nano topography of calcium phosphates strongly affected the protein adsorption process, being more important than surface chemistry. The seeding efficacy of osteoblasts was not affected nor by the topography neither by the calcium phosphate chemistries but the β-TCP chemistry negatively influenced cell spreading. We observed that surface hydrophobicity is another way to change protein adsorption on surfaces. The decrease of the polar component of surface energy on gold-coated samples leaded to a decreased albumin and fibronectin adsorption but to an increased cell adhesion. Overall, this work contributes to better understand the role of topography and surface chemistry of calcium phosphates in serum protein adsorption and osteoblast adhesion.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. L. HAO and J. LAWRENCE, Colloid. Surface B 34 (2004) 87

    Article  CAS  Google Scholar 

  2. S. B. KENNEDY, N. R. WASHBURN, C. G. SIMON and E. J. AMIS, Biomaterials 27 (2006) 3817

    Article  CAS  Google Scholar 

  3. K. L. KILPADI, P. L. CHANG and S. L. BELLIS, J. Biomed. Mater. Res. 57 (2001) 258

    Article  CAS  Google Scholar 

  4. C. YONGLI, Z. XIUFANG, G. YANDAO, Z. NANMING, Z. TINGYING and S. XINQI, J. Colloid Interf. Sci. 214 (1999) 38

    Article  CAS  Google Scholar 

  5. R. G. LEBARON and K. A. ATHANASIOU, Tissue Eng. 6 (2000) 85

    Article  CAS  Google Scholar 

  6. A. A. SAWYER, K. M. HENESSY and S. L. BELLIS, Biomaterials 26 (2005) 1467

    Article  CAS  Google Scholar 

  7. D. T. HUGHES WASSELL, R. C. HALL and G. EMBERY, Biomaterials 16 (1995) 697

    Article  Google Scholar 

  8. Q. LUO and J. D. ANDRADE, J. Colloid Interf. Sci. 200 (1998) 104

    Article  CAS  Google Scholar 

  9. K. CAI, J. BOSSERT and K. D. JANDT, Colloid. Surface B 49 (2006) 136

    Article  CAS  Google Scholar 

  10. B. WOJCIAK-STOTHARD, A. CURTIS, W. MONAGHAN, K. MACDONALD and C. WILKINSON, Exp. Cell. Res. 223 (1996) 426

    Article  CAS  Google Scholar 

  11. Y. F. DUFRÊNE, T. G. MARCHAL and P. G. ROUXHET, Langmuir 15 (1999) 2871

    Article  Google Scholar 

  12. S. G. PENN, L. HE and M. J. NATAN, Curr. Opin. Cell Biol. 7 (2003) 609

    CAS  Google Scholar 

  13. S. V. DOROZHKIN and M. EPPLE, Angew. Chem. Int. Ed. Engl. 41 (2002) 3130

    Article  CAS  Google Scholar 

  14. A. L. OLIVEIRA, P. B. MALAFAYA and R. L. REIS, Biomaterials 24 (2003) 2575

    Article  CAS  Google Scholar 

  15. K. BENZARTI, C. PERRUCHOT and M. M. CHEHIMI, Colloid. Surface A 286 (2006) 78

    Article  CAS  Google Scholar 

  16. D. K. OWENS and R. C. WENDT, J. Appl. Polym. Sci. 13 (1969) 1741

    Article  CAS  Google Scholar 

  17. M. J. DALBY, Med. Eng. Phys. 27 (2005) 730

    Article  Google Scholar 

  18. L. PONSONNET, V. COMTE, A. OTHMANE, C. LAGNEAU, M. CHARBONNIER, M. LISSAC and N. JAFFREZIC, Mat. Sci. Eng. C 21 (2002) 157

    Article  Google Scholar 

  19. J. Y. WONG, J. B. LEACH and X. Q. BROWN, Surf. Sci. 570 (2004) 119

    Article  CAS  Google Scholar 

  20. S. RAYNAUD, E. CHAMPION and D. BERNACHE-ASSOLANT, Biomaterials 23 (2002) 1073

    Article  CAS  Google Scholar 

  21. H. S. RYU, H. J. YOUN, K. S. HONG, B. S. CHANG, C. K. LEE and S. S. CHUNG, Biomaterials 23 (2002) 909

    Article  CAS  Google Scholar 

  22. P. MIRANDA, E. SAIZ, K. GRYN and A. P. TOMSIA, Acta Biomater. 2 (2006) 457

    Article  Google Scholar 

  23. S. BAILLIEZ and A. NZIHOU, Chem. Eng. J. 98 (2004) 141

    Article  CAS  Google Scholar 

  24. J. LONG, M. N. HYDER, R. Y. HUANG and P. CHEN, Adv. Colloid Interf. Sci. 118 (2005) 173

    Article  CAS  Google Scholar 

  25. M. HAN, A. SETHURAMAN, R. S. KANE and G. BELFORT, Langmuir 19 (2003) 9868

    Article  CAS  Google Scholar 

  26. F. L. YAP and Y. ZHANG, Biosens. Bioelectron. 22 (2007) 775

    Article  CAS  Google Scholar 

  27. A. DABROWSKI, Adv. Colloid Interf. Sci. 93 (2001) 135

    Article  CAS  Google Scholar 

  28. N. J. HALLAB, K. J. BUNDY, K. O’CONNOR, R. L. MOSES and J. J. JACOBS, Tissue Eng. 7 (2001) 55

    Article  CAS  Google Scholar 

  29. T. G. Van KOOTEN, H. T. SPIJKER and H. J. BUSSCHER, Biomaterials 25 (2004) 1735

    Article  Google Scholar 

  30. B. G. KESELOWSKY, D. M. COLLARD and A. J. GARCIA, J. Biomed. Mater. Res. 66A (2002) 247

    Article  Google Scholar 

  31. G. K. TOWORFE, R. J. COMPOSTO, C. S. ADAMS, I. M. SHAPIRO and P. DUCHEYNE, J. Biomed. Mater. Res. 71 (2004) 449

    Article  Google Scholar 

  32. K. WEBB, V. HLADY and P. A. TRESCO, J. Biomed. Mater. Res. 41 (1998) 422

    Article  CAS  Google Scholar 

  33. S. J. LEE, G. KHANG, Y. M. LEE and H. B. LEE, J. Colloid Interf. Sci. 259 (2003) 228

    Article  CAS  Google Scholar 

  34. J. H. LEE, S. J. LEE, G. KHANG and H. B. LEE, J. Colloid Interf. Sci. 230 (2000) 84

    Article  CAS  Google Scholar 

  35. L. MHAMDI, C. PICART, C. LAGNEAU, A. OTHMANE, B. GROGOGEAT, N. JAFFREZIC, L. PONSONNET, Mat. Sci. Eng. C 26 (2006) 273

    Article  CAS  Google Scholar 

  36. X. LIU, J. Y. LIM, H. J. DONAHUE, R. DHURJATI, A. M. MASTRO and E. A. VOGLER, Biomaterials 28 (2007) 4535

    Article  CAS  Google Scholar 

  37. H. M. KOWALCZYNSKA, M. NOWAK-WYRZYKOWSKA, R. KOLOS, J. DOBKOWSKI and J. KAMINSKY, J. Biomed. Mater. Res. 72 (2005) 228

    Article  Google Scholar 

  38. H. M. KOWALCZYNSKA and M. NOWAK-WYRZYKOWSKA, Cell Biol. Int. 27 (2003) 101

    Article  CAS  Google Scholar 

  39. S. A. REDEY, M. NARDIN, D. BERNACHE-ASSOLANT, C. REY, P. DELANNOY, L. SEDEL and P. J. MARIE, J. Biomed. Mater. Res. 50 (2000) 353

    Article  CAS  Google Scholar 

  40. G. ALTANKOV and T. GROTH, J. Mater. Sci. Mater. Med. 5 (1994) 732

    Article  CAS  Google Scholar 

  41. L. PONSONNET, K. REYBIER, N. JAFFREZIC, V. COMTE, C. LAGNEAU, M. LISSAC and C. MARTELET, Mat. Sci. Eng. C 23 (2003) 551

    Article  Google Scholar 

  42. A. S. SANTIAGO, E. A. DOS SANTOS, M. S. SADER, M. S. SANTIAGO and G. A. SOARES, Braz. Oral. Res. 19 (2005) 203

    Article  Google Scholar 

  43. R. QUIRK, W. C. CHAN, M. C. DAVIES, S. J. B. TENDLER and K. M. SHAKESHEFF, Biomaterials 22 (2001) 865

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research is part of the SWE/DSc program of CNPq Brazilian research agency. The authors acknowledge the financial support given by CNPq/Brazil and CNRS/France. They also thank the Catalysis Group NUCAT/COPPE/UFRJ and CBPF/Brazil.

Author information

Authors and Affiliations

  1. Dep. de Eng. Metal. e de Materiais, COPPE/UFRJ, CP 68505, Rio de Janeiro, 21941-972, Brazil

    E. A. dos Santos & G. A. Soares

  2. Institut de Chimie des Surfaces et Interfaces/UPR CNRS 9069, 15 rue Jean Starcky, BP 2488, 68057, Mulhouse, France

    E. A. dos Santos & K. Anselme

  3. Lab. de Biomineralização, ICB/UFRJ, Rio de Janeiro, 21941-590, Brazil

    M. Farina

Authors
  1. E. A. dos Santos
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Farina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. A. Soares
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. Anselme
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to K. Anselme.

Rights and permissions

Reprints and permissions

About this article

Cite this article

dos Santos, E.A., Farina, M., Soares, G.A. et al. Surface energy of hydroxyapatite and β-tricalcium phosphate ceramics driving serum protein adsorption and osteoblast adhesion. J Mater Sci: Mater Med 19, 2307–2316 (2008). https://doi.org/10.1007/s10856-007-3347-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-007-3347-4

Keywords

Search

Navigation