Biocompatibility of polyhydroxybutyrate microspheres: in vitro and in vivo evaluation

  • Ekaterina Igorevna Shishatskaya
  • Olga N. Voinova
  • Anastasiya V. Goreva
  • Olga A. Mogilnaya
  • Tatiana G. Volova


Microspheres have been prepared from the resorbable linear polyester of β-hydroxybutyric acid (polyhydroxybutyrate, PHB) by the solvent evaporation technique and investigated in vitro and in vivo. Biocompatibility of the microspheres has been proved in tests in the culture of mouse fibroblast cell line NIH 3T3 and in experiments on intramuscular implantation of the microspheres to Wistar rats for 3 months. Tissue response to the implantation of polymeric microspheres has been found to consist in a mild inflammatory reaction, pronounced macrophage infiltration that increases over time, involving mono- and poly-nuclear foreign body giant cells that resorb the polymeric matrix. No fibrous capsules were formed around polymeric microparticles; neither necrosis nor any other adverse morphological changes and tissue transformation in response to the implantation of the PHB microparticles were recorded. The results of the study suggest that polyhydroxybutyrate is a good candidate for fabricating prolonged-action drugs in the form of microparticles intended for intramuscular injection.


PHAs PHBV Fibrous Capsule Foreign Body Giant Cell Polyhydroxybutyrate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This study was financially supported by the Russian Ministry of Education and the U.S. Civilian Research & Development Foundation CRDF (Grant No. P1MO002), the Program of the RAS Presidium “Fundamental Research to Medicine” (Project No. 12.5), and the Program of the President of Russia (Grant MK-4149.2006.4).


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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Ekaterina Igorevna Shishatskaya
    • 1
  • Olga N. Voinova
    • 1
  • Anastasiya V. Goreva
    • 1
  • Olga A. Mogilnaya
    • 2
  • Tatiana G. Volova
    • 1
    • 3
  1. 1.Laboratory of Chemoautotrophic BiosynthesisInstitute of Biophysics SB RAS (Siberian Branch Russian Academy of Sciences)KrasnoyarskRussia
  2. 2.Laboratory of Bacterial BioluminescenceInstitute of Biophysics SB RAS (Siberian Branch Russian Academy of Sciences)KrasnoyarskRussia
  3. 3.Institute of Fundamental Biology and BiotechnologySiberian Federal UniversityKrasnoyarskRussia

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