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In vitro evaluation of poly(caporlactone) grafted dextran (PGD) nanoparticles with cancer cell

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Abstract

This study dealt with the preparation and characterization of coumarin-6 loaded poly(caprolactone) grafted dextran (PGD) nanoparticles (NPs) and evaluation of cellular uptake by using human gastric cancer cell line (SNU-638), in vitro. The potential application of these PGD NPs for sustained drug delivery was evaluated by the quantification and localization of the cellular uptake of fluorescent PGD NPs. Coumarin-6 loaded PGD NPs were prepared by a modified oil/water emulsion technique and characterized by various physico-chemical methods such as, laser light scattering for particle size and size distribution, atomic force microscopy (AFM), zeta-potential and spectrofluorometry to identify the release of fluorescent molecules from the NPs. SNU-638 was used to measure the cellular uptake of fluorescent PGD NPs. Confocal laser scanning microscopic images clearly showed the internalization of NPs by the SNU-638 cells. Cell viability was assessed by treating the SNU-638 cells with PGD NPs for 48 h. The results reveal, that these biodegradable polymeric NPs holds promise in biomedical field as a carrier.

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Acknowledgement

This work was supported by the Korean Research Foundation Grant founded by the Korean Government (MOEHRD) (The Center for Health Care Technology Development, Chonbuk National University, Jeonju 561-756 Republic of Korea) and Korean Government Project No: 10028211, through Ministry of Commerce, Industry and Energy Department.

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Correspondence to H. Y. Kim.

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Prabu, P., Chaudhari, A.A., Aryal, S. et al. In vitro evaluation of poly(caporlactone) grafted dextran (PGD) nanoparticles with cancer cell. J Mater Sci: Mater Med 19, 2157–2163 (2008). https://doi.org/10.1007/s10856-007-3307-z

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