In vitro evaluation of poly(caporlactone) grafted dextran (PGD) nanoparticles with cancer cell
This study dealt with the preparation and characterization of coumarin-6 loaded poly(caprolactone) grafted dextran (PGD) nanoparticles (NPs) and evaluation of cellular uptake by using human gastric cancer cell line (SNU-638), in vitro. The potential application of these PGD NPs for sustained drug delivery was evaluated by the quantification and localization of the cellular uptake of fluorescent PGD NPs. Coumarin-6 loaded PGD NPs were prepared by a modified oil/water emulsion technique and characterized by various physico-chemical methods such as, laser light scattering for particle size and size distribution, atomic force microscopy (AFM), zeta-potential and spectrofluorometry to identify the release of fluorescent molecules from the NPs. SNU-638 was used to measure the cellular uptake of fluorescent PGD NPs. Confocal laser scanning microscopic images clearly showed the internalization of NPs by the SNU-638 cells. Cell viability was assessed by treating the SNU-638 cells with PGD NPs for 48 h. The results reveal, that these biodegradable polymeric NPs holds promise in biomedical field as a carrier.
KeywordsAtomic Force Microscopy Drug Release Dextran Cellular Uptake Caprolactone
This work was supported by the Korean Research Foundation Grant founded by the Korean Government (MOEHRD) (The Center for Health Care Technology Development, Chonbuk National University, Jeonju 561-756 Republic of Korea) and Korean Government Project No: 10028211, through Ministry of Commerce, Industry and Energy Department.
- 1.Cancer nanotechnology; US Department of Health & Human Service, National Institute of Health and National Cancer Institute, 2004Google Scholar
- 3.K. PETRAK, Drugs Pharm. Sci. 61 (1993) 275Google Scholar
- 5.C. G. Pitt, New York. Marcel Dekker (1990) 71Google Scholar
- 10.G. GREGORIADIS, in “Drug Carriers in Biology and Medicine” (Academic Press, London, 1979) p. 107Google Scholar
- 11.M. J. POZNANSKY and L. G. CLELAND, in “Drug Delivery Systems” (Oxford University Press, New York, 1980) p. 253Google Scholar
- 12.T. W. SERY and E. J. HEHRE, J. Bacterio. 71 (1956) 71Google Scholar
- 30.P. ARTURSSON, Ther. Drug Carrier Syst. 8 (1991) 305Google Scholar
- 32.M. BUTLER, in “Animal Cell Culture and Technology” (Oxford, IRL Press, 1996) p. 112Google Scholar