In vitro evaluation of poly(caporlactone) grafted dextran (PGD) nanoparticles with cancer cell

  • P. Prabu
  • Atul A. Chaudhari
  • Santosh Aryal
  • N. Dharmaraj
  • S. Y. Park
  • W. D. Kim
  • H. Y. Kim


This study dealt with the preparation and characterization of coumarin-6 loaded poly(caprolactone) grafted dextran (PGD) nanoparticles (NPs) and evaluation of cellular uptake by using human gastric cancer cell line (SNU-638), in vitro. The potential application of these PGD NPs for sustained drug delivery was evaluated by the quantification and localization of the cellular uptake of fluorescent PGD NPs. Coumarin-6 loaded PGD NPs were prepared by a modified oil/water emulsion technique and characterized by various physico-chemical methods such as, laser light scattering for particle size and size distribution, atomic force microscopy (AFM), zeta-potential and spectrofluorometry to identify the release of fluorescent molecules from the NPs. SNU-638 was used to measure the cellular uptake of fluorescent PGD NPs. Confocal laser scanning microscopic images clearly showed the internalization of NPs by the SNU-638 cells. Cell viability was assessed by treating the SNU-638 cells with PGD NPs for 48 h. The results reveal, that these biodegradable polymeric NPs holds promise in biomedical field as a carrier.


Atomic Force Microscopy Drug Release Dextran Cellular Uptake Caprolactone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by the Korean Research Foundation Grant founded by the Korean Government (MOEHRD) (The Center for Health Care Technology Development, Chonbuk National University, Jeonju 561-756 Republic of Korea) and Korean Government Project No: 10028211, through Ministry of Commerce, Industry and Energy Department.


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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • P. Prabu
    • 1
  • Atul A. Chaudhari
    • 2
  • Santosh Aryal
    • 3
  • N. Dharmaraj
    • 4
  • S. Y. Park
    • 2
  • W. D. Kim
    • 5
  • H. Y. Kim
    • 6
  1. 1.Department of Bionanosystem EngineeringChonbuk National UniversityJeonjuRepublic of Korea
  2. 2.College of Veterinary MedicineChonbuk National UniversityJeonjuRepublic of Korea
  3. 3.Center for Healthcare Technology DevelopmentChonbuk National UniversityJeonjuRepublic of Korea
  4. 4.Department of ChemistryBharathiar UniversityCoimbatoreIndia
  5. 5.Department of Future TechnologyKorea Institute of Machinery and MaterialsDaejeonRepublic of Korea
  6. 6.Department of Textile EngineeringChonbuk National UniversityJeonjuRepublic of Korea

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