Genipin-crosslinked chitosan/gelatin blends for biomedical applications

  • Valeria Chiono
  • Ettore Pulieri
  • Giovanni Vozzi
  • Gianluca Ciardelli
  • Arti Ahluwalia
  • Paolo Giusti


Blends between chitosan (CS) and gelatin (G) with various compositions (CS/G 0/100 20/80, 40/60, 60/40 100/0 w/w) were produced, as candidate materials for biomedical applications. Different amounts of genipin (0.5 wt.% and 2.5 wt.%) were used to crosslink CS/G blends, promoting the formation of amide and tertiary amine bonds between the macromolecules and the crosslinker. The effects of composition and crosslinking on the physico-chemical properties of samples were evaluated by infrared analysis, thermogravimetry, contact angle measurements, dissolution and swelling tests. Mechanical properties of crosslinked samples were also determined through stress–strain and creep tests: samples stiffness increased with increasing the crosslinker amount and the CS content. Blend composition affected mouse fibroblasts adhesion and proliferation on substrates, depending on the crosslinker amount. Finally, crosslinked CS/G blends containing 80 wt.% G were found to support neuroblastoma cells adhesion and proliferation which made them promising candidates for uses in the field of nerve regeneration.


Contact Angle Genipin Geniposide Peripheral Nerve Regeneration Crosslinking Degree 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Valeria Chiono
    • 1
  • Ettore Pulieri
    • 2
  • Giovanni Vozzi
    • 1
    • 2
  • Gianluca Ciardelli
    • 1
    • 3
  • Arti Ahluwalia
    • 2
  • Paolo Giusti
    • 1
    • 4
  1. 1.Department of Chemical Engineering, Industrial Chemistry and Materials ScienceUniversity of PisaPisaItaly
  2. 2.Centro “E. Piaggio”University of PisaPisaItaly
  3. 3.Department of MechanicsPolitecnico in TurinTurinItaly
  4. 4.C.N.R. Institute for Composite and Biomedical MaterialsPisaItaly

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