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Genipin-crosslinked chitosan/gelatin blends for biomedical applications

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Abstract

Blends between chitosan (CS) and gelatin (G) with various compositions (CS/G 0/100 20/80, 40/60, 60/40 100/0 w/w) were produced, as candidate materials for biomedical applications. Different amounts of genipin (0.5 wt.% and 2.5 wt.%) were used to crosslink CS/G blends, promoting the formation of amide and tertiary amine bonds between the macromolecules and the crosslinker. The effects of composition and crosslinking on the physico-chemical properties of samples were evaluated by infrared analysis, thermogravimetry, contact angle measurements, dissolution and swelling tests. Mechanical properties of crosslinked samples were also determined through stress–strain and creep tests: samples stiffness increased with increasing the crosslinker amount and the CS content. Blend composition affected mouse fibroblasts adhesion and proliferation on substrates, depending on the crosslinker amount. Finally, crosslinked CS/G blends containing 80 wt.% G were found to support neuroblastoma cells adhesion and proliferation which made them promising candidates for uses in the field of nerve regeneration.

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Authors and Affiliations

  1. Department of Chemical Engineering, Industrial Chemistry and Materials Science, University of Pisa, Via Diotisalvi 2, Pisa, 56126, Italy

    Valeria Chiono, Giovanni Vozzi, Gianluca Ciardelli & Paolo Giusti

  2. Centro “E. Piaggio”, University of Pisa, Via Diotisalvi 2, Pisa, 56126, Italy

    Ettore Pulieri, Giovanni Vozzi & Arti Ahluwalia

  3. Department of Mechanics, Politecnico in Turin, Corso Duca degli Abruzzi 24, Turin, 10129, Italy

    Gianluca Ciardelli

  4. C.N.R. Institute for Composite and Biomedical Materials, Via Diotisalvi 2, Pisa, 56126, Italy

    Paolo Giusti

Authors
  1. Valeria Chiono
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  2. Ettore Pulieri
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  3. Giovanni Vozzi
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  4. Gianluca Ciardelli
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  5. Arti Ahluwalia
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Correspondence to Valeria Chiono.

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Chiono, V., Pulieri, E., Vozzi, G. et al. Genipin-crosslinked chitosan/gelatin blends for biomedical applications. J Mater Sci: Mater Med 19, 889–898 (2008). https://doi.org/10.1007/s10856-007-3212-5

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  • DOI: https://doi.org/10.1007/s10856-007-3212-5

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