Encapsulation and controlled release of lysozyme from electrospun poly(ε-caprolactone)/poly(ethylene glycol) non-woven membranes by formation of lysozyme–oleate complexes

  • Yan Li
  • Hongliang Jiang
  • Kangjie Zhu


In this study, the concept of hydrophobic ion pairing was adopted for incorporating lysozyme into electrospun poly(ε-caprolactone) (PCL)/poly(ethylene glycol) (PEG) non-woven membranes. The solubility of lysozyme in organic solvent was enhanced through the formation of lysozyme–oleate complexes, which could be directly loaded into PCL/PEG membranes using electrospinning technique. The resultant PCL/PEG nanofibers have a compact structure with an average diameter ranged from about 0.4 μm to 0.9 μm. The addition of PEG into the PCL nanofibers not only improved the hydrophilicity of the membrane, but also played an important role on in vitro lysozyme release rate. It was found that the release rate of lysozyme was enhanced with the increase of PEG content. In addition, the increase of salt concentration in the release medium accelerated lysozyme release. It has also been shown that the released lysozyme retained most of its enzymatic activity.


Lysozyme Electrospun Fiber Sodium Oleate Electrospun Membrane Electrospun Poly 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors would like to thank the support of NATIONAL NATURAL SCIENCE FOUNDATION OF CHINA (Project 20304013).


  1. 1.
    M. BOGNITZKI, W. CZADO, T. FRESE, A. SCHAPER, M. HELLWIG, M. STEINHART, A. GREINER and J. H. WENDORFF, Adv. Mater. 13 (2001) 70CrossRefGoogle Scholar
  2. 2.
    D. S. KATTI, K. W. ROBINSON, F. K. KO and C. T. LAURENCIN, J. Biomed. Mater. Res. Part B: Appl. Biomater. 70B (2004) 286CrossRefGoogle Scholar
  3. 3.
    W. J. LI, C. T. LAURENCIN, E. J. CATERSON, R. S. TUAN and F. K. KO, J. Biomed. Mater. Res. 60 (2002) 613CrossRefGoogle Scholar
  4. 4.
    H. YOSHIMOTOA, Y. M. SHINA, H. TERAIA and J. P. VACANTIA, Biomaterials 24 (2003) 2077CrossRefGoogle Scholar
  5. 5.
    K. KIM, M. YU, X. H. ZONG, J. CHIU, D. F. FANG, Y. S. SEO, B. S. HSIAO, B. CHU and M. HADJIARGYRON, Biomaterials 24 (2003) 4977CrossRefGoogle Scholar
  6. 6.
    S. R. BHATTARAI, N. BHATTARAI, H. K. YI, P. H. HWANG, D. I. CHAD and H. Y. KIM, Biomaterials 25 (2004) 2595CrossRefGoogle Scholar
  7. 7.
    W. J. LI, R. TULI, C. OKAFOR, A. DERFOUL, K. G. DANIELSON, D. J. HALL and R. S. TUAN, Biomaterials 26 (2005) 599CrossRefGoogle Scholar
  8. 8.
    Y. K. LUU, K. KIM, B. S. HSIAO, B. CHU and M. HADJIARGYRON, J. Control. Release 89 (2003) 341CrossRefGoogle Scholar
  9. 9.
    J. ZENG, X. Y. XU, X. S. CHEN, Q. Z. LIANG, X. C. BIAN, L. X. YANG and X. B. JING, J. Control. Release 92 (2003) 227CrossRefGoogle Scholar
  10. 10.
    E. R. KENAWY, G. L. BOWLIN, K. MANSFIELD, J. LAYMAN, D. G. SIMPSON, E. H. SANDERS and G. E. WNEK, J. Control. Release 81 (2002) 57CrossRefGoogle Scholar
  11. 11.
    M. E. BREWSTER, G. VERRECK, I. CHUN, J. ROSENBLATT, J. MENSCH, D. A. VAN, M. NOPPE, A. ARIEN, M. BRUINING and J. PEETERS, Pharmazie 59 (2004) 387Google Scholar
  12. 12.
    H. L. JIANG, D. F. FANG, B. HSIAO, B. CHU and W. CHEN, J. Biomater. Sci. Polymer Edn. 15 (2004) 279CrossRefGoogle Scholar
  13. 13.
    K. KIM, Y. K. LUU, C. CHANG, D. F. FANG, B. S. HSIAO, B. CHU and M. HADJIARGYRON, J. Control. Release 98 (2004) 47CrossRefGoogle Scholar
  14. 14.
    H. L. JIANG, D. F. FANG, B. HSIAO, B. CHU and W. CHEN, Biomacromolecules 5 (2004) 326CrossRefGoogle Scholar
  15. 15.
    E. H. SANDERS, R. KLOEFKORN, G. L. BOWLIN, D. G. SIMPSON and G. E. WNEK, Macromolecules 36 (2003) 3803CrossRefGoogle Scholar
  16. 16.
    H. S. YOO, H. K. CHOI and T. G. PARK, J. Pharm. Sci. 90 (2001) 194CrossRefGoogle Scholar
  17. 17.
    K. HOLMBERG, B. JÖNSSON, B. KRONBERG and B. LINDMAN, in “Surfactants and Polymers in Aqueous Solution. Chapter 14: surfactant-protein mixtures” (John Wiley & Sons, Ltd., New York, 2002) p. 305Google Scholar
  18. 18.
    K. H. LEE, H. Y. KIM, M. S. KHIL, Y. M. RA and D. R. LEE, Polymer 44 (2003) 1287CrossRefGoogle Scholar
  19. 19.
    H. A. MCKENZIE and F. W. WHITE, Anal. Biochem. 157 (1986) 367CrossRefGoogle Scholar
  20. 20.
    W. J. LIN and H. G. LEE, J. Control. Release 89 (2003) 179CrossRefGoogle Scholar
  21. 21.
    W. P. YE, F. S. DU, W. H. JIN, J. Y. YANG and Y. XU, React. Funct. Polym. 32 (1997) 161CrossRefGoogle Scholar
  22. 22.
    C. PÉREZ, P. D. JESÚS and K. GRIEBENOW, Int. J. Pharm. 248 (2002) 193CrossRefGoogle Scholar
  23. 23.
    W. WANG, Int. J. Pharm. 185 (1999) 129CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  1. 1.Department of Polymer Science and EngineeringZhejiang UniversityHangzhouChina

Personalised recommendations