Characterization and osteoblast-like cell compatibility of porous scaffolds: bovine hydroxyapatite and novel hydroxyapatite artificial bone
- 307 Downloads
Three different porous scaffolds were tested. The first two were prepared by sintering bovine bone. The third scaffold was prepared using three-dimensional gel-lamination, a new rapid prototyping method, and was named as hydroxyapatite artificial bone.
X-ray diffraction and Fourier transform infrared spectroscopy analysis confirmed that the samples were mainly highly crystalline hydroxyapatite ceramics. Scanning electron microscopy and mercury intrusion porosimetry measurement showed that the pores were interconnected and pore sizes ranged from several microns to hundreds of microns.
Mouse osteoblast-like cells grown on the three scaffolds retained their characteristic morphology. Cell proliferation and differentiation, analyzed by methylthiazol tetrazolium (MTT) and alkaline phosphatase activity assays, were significantly higher on the hydroxyapatite artificial bone than on the other two scaffolds tested. All the scaffolds provided good attachment, proliferation and differentiation of bone cells.
These results indicate that the scaffolds have a favorable interaction with cells, they support cell growth and functions, and therefore these scaffolds may have great potential as bone substitutes. The three-dimensional gel-lamination method is proven to be an attractive process to design and fabricate bone scaffolds with favorable properties, and therefore, has promising potential for bone repair applications.
KeywordsPorous Scaffold Mercury Intrusion Porosimetry Bovine Bone Bone Scaffold Calcium Nitrate Tetrahydrate
Unable to display preview. Download preview PDF.
- 2.M. JARCHO, Clin. Orthop. Rel. Res. 157 (1981) 259.Google Scholar
- 3.H. YAMASAKI, Jpn. J. Oral. Biol. 32 (1990) 190.Google Scholar
- 4.U. RIPAMONTI, J. Bone. Joint. Surg. Am. 73 (1991) 692.Google Scholar
- 5.X. D. ZHANG, In “Bioceramics and the human body” (Amsterdam, Elsevier, 1991) p. 408.Google Scholar
- 6.H. YUAN and Y. LI Biomed. Eng. Appl. Basis. Com. 9 (1997) 274.Google Scholar
- 10.P. SEPULVEDA, Am. Ceram. Soc. Bull. 76 (1997) 61.Google Scholar
- 11.J. SAGGIO-WOYANSKY and C. E. SCOTT Am. Ceram. Soc. Bull. 71 (1992) 1674.Google Scholar
- 23.J. F. OSBORN, In “Implantatwerkstoff Hydroxylapatitkeramik” (Berlin, Quintessenz Verlags-GmbH, 1985) p. 17–8; 32–6; 39.Google Scholar
- 27.P. S. EGGLI and W. MULLER Clin. Orthop. Rel. Res. 232 (1988) 127.Google Scholar
- 28.R. E. HOLMES and V. MOONEY Clin. Orthop. Rel. Res. 188 (1984) 252.Google Scholar
- 32.E. TSURUGA and H. TAKITA J. Biochem (Tokyo). 121 (1997) 317.Google Scholar
- 37.L. J. GIBSON and M. F. ASHBY, In “Cellular solids: structure and properties. Cambridge solid state science series, 2 nd Ed” (Cambridge, UK, Cambridge University Press, 1997) p. 429.Google Scholar
- 39.P. J. MARIE, Calcif. Tissue. Int. 56 Suppl 1 (1995) S13.Google Scholar