Poly(2-hydroxyethyl methacrylate) microspheres/liquid poly(dimethylsiloxane) composition for correction of small defects in face: Histological evaluation in animal experiment

  • D. Horák
  • A. Adamyan
  • O. Golubeva
  • N. Skuba
  • T. Vinokurova


Two kinds of composition based on commercial liquid poly(dimethylsiloxane) and laboratory-made poly(2-hydroxyethyl methacrylate) (PHEMA) microspheres of different size fractions (30–40 or 125–180 μ m) were prepared. Tissue reaction on injection of the compositions, optimum microsphere size and morphology were investigated in the experiments on rats. The microspheres induced foreign body reaction characterized by an increased content of fibroblasts and mild infiltration of injection field by inflammatory cells. The 125–180 μ m microspheres seemed to be well covered with poly(dimethylsiloxane) and more uniformly distributed in the tissue than the 30–40 μ m ones. As a result, the extent of foreign body reaction induced by the former microspheres was somewhat lower than that induced by the latter. Moreover, time-dependent degradation of 30–40 μ m PHEMA microspheres was more pronounced than that of 125–180 μ m ones, which can affect duration of the aesthetic effect after prospective facioplasty. Results of histological investigations demonstrate a good prospect of the proposed composition for contour and bulk facioplasty of small soft tissue defects and skin wrinkles.


Size Fraction Histological Evaluation Tissue Reaction Small Defect Foreign Body Reaction 
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  1. 1.
    D. A. ELLIS, A. S. MAKDESSIAN and D. J. BROWN, Facial Plast. Surg. Clin. North Am. 9 (2001) 405.Google Scholar
  2. 2.
    L. A. BRUSOVA and N. I. OSTRECOVA, Ann. Plast. Reconstruct. Aesthetic Surg. 2 (2001) 52.Google Scholar
  3. 3.
    M. MC CLELLAND, B. EGBERT, V. HANKO, R. A. BERG and F. DE LUSTRO, Plast. Reconstr. Surg. 100 (1997) 1466.Google Scholar
  4. 4.
    A. I. NEROBEEV, G. I. OSOPIV, V. I. MALACHOVSKAYA and A. L. ISCHENKO, An. Plast. Reconstruct. Aesthetic Surg. 2 (1997) 22.Google Scholar
  5. 5.
    M. OLENIUS, Aesthetic Plast Surg. 22 (1998) 97.CrossRefGoogle Scholar
  6. 6.
    I. PITANGUY, P. MULLER, N. PICCOLO and L. FREITAS, Compendium 8 (1987) 460.Google Scholar
  7. 7.
    J. T. CHENG, S. W. PERKINS and M. M. HAMILTON, Otolaryngol. Clin. North Am. 35 (2002) 73.CrossRefGoogle Scholar
  8. 8.
    D. PIACQUADIO, M. JARCHO and R. GOLTZ, J. Am. Acad. Dermatol. 36 (1997) 544.CrossRefGoogle Scholar
  9. 9.
    G. LEMPERLE, J. J. ROMANO and M. BUSSO, Dermatol. Surg. 29 (2003) 573.CrossRefGoogle Scholar
  10. 10.
    C. BERGERET-GALLEY, X. LATOUCHE and Y. G. ILLOUZ, Aesthetic Plast. Surg. 25 (2001) 249.CrossRefGoogle Scholar
  11. 11.
    F. L. ASHLEY, S. BRALEY, T. D. REES, D. GOULIAN and D. L. BALLANTYNE Jr., Plast. Reconstr. Surg. 39 (1967) 411.Google Scholar
  12. 12.
    T. D. REES, D. L. BALLANTYNE Jr., I. SEIDMAN and G. A. HAWTHORNE, Plast. Reconstr. Surg. 39 (1967) 402.Google Scholar
  13. 13.
    M. T. EDGERTON, Clin. Plast. Surg.4 (1947) 311.Google Scholar
  14. 14.
    A. A. ADAMYAN, N. I. OSTRECOVA, T. M. CZUDINOVA, R. C. MAGOMADOV and K. O. SULTANOVA, Ann. Plast. Reconstruct. Aesthetic Surg. 2 (1997) 41.Google Scholar
  15. 15.
    T. J. SERGOTT, J. P. LIMOLI, C. M BALDWIN Jr. and D. R. LAUB, Plast. Reconstr. Surg. 78 (1986) 104.Google Scholar
  16. 16.
    R. SHAFIR, A. AMIR and E. GUR, Plast. Reconstr. Surg. 106 (2000) 1215.Google Scholar
  17. 17.
    N. A. PEPPAS (ed.) Hydrogels in Medicine and Pharmacy (CRC Press, Boca Raton, 1987) Vol. III.Google Scholar
  18. 18.
    D. HORÁK, M. ČERVINKA and V. PŮŽA, Biomaterials 18 (1997) 1355.Google Scholar
  19. 19.
    O. A. GOLUBEVA, HEMASIL—A new composite injection material for correction of small defects in face (in Russian), PhD. Thesis, Vishnevskii Institute of Surgery, Moscow 2003.Google Scholar
  20. 20.
    J. ČOUPEK, M. KŘIVÁKOVÁ and S. POKORNÝ, J. Polym. Sci. Polym. Symp. 42 (1973) 185.Google Scholar
  21. 21.
    D. HORÁK, F. LEDNICKÝ, V. ŘEHÁK and F. ŠVEC, J. Appl. Polym. Sci. 49 (1993) 2041.CrossRefGoogle Scholar
  22. 22.
    D. HORÁK, F. ŠVEC, J. KÁLAL, K. Z. GUMARGALIEVA, A. A. ADAMYAN, N. D. SKUBA, M. I. TITOVA and N. V. TROSTENYUK, Biomaterials 7 (1986) 188.Google Scholar
  23. 23.
    K. F. MUELLER, S. J. HEIBER and W. L. PLANKL, Process for preparing hydrogels as spherical beads of large size, US Patent 4, 224, 427 (1980).Google Scholar
  24. 24.
    O. A. GOLUBEVA and A. V. NIKOLAEVA-FEDOROVA, Vestnik Ross. Gosudarstv. Mosk. Univ. 28 (2003) 44.Google Scholar

Copyright information

© Springer Science + Business Media, Inc. 2006

Authors and Affiliations

  • D. Horák
    • 1
    • 2
  • A. Adamyan
    • 3
  • O. Golubeva
    • 3
  • N. Skuba
    • 3
  • T. Vinokurova
    • 3
  1. 1.Institute of Macromolecular ChemistryAcademy of Sciences of the Czech RepublicPrague 6Czech Republic
  2. 2.Center for Cell Therapy and Tissue Repair, 2nd Medical FacultyCharles UniversityPrague 5Czech Republic
  3. 3.Vishnevskii Institute of SurgeryAcademy of Medical SciencesMoscowRussia

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