Genotoxicity of dental resin polymerization initiators in vitro

  • Y. Nomura
  • W. Teshima
  • T. Kawahara
  • N. Tanaka
  • H. Ishibashi
  • M. Okazaki
  • K. Arizono


The polymerization initiators for resins cured using visible light usually consist of a photosensitizer, primarily camphorquinone (CQ), and a reducing agent, which is often a tertiary amine (DMPT, DMAEMA), while the initiator used for self-curing resins consists of benzoyl peroxide (BPO) and a tertiary amine (DMPT). The genotoxicities of camphorquinone (CQ), benzoyl peroxide (BPO), dimethyl-para-toluidine (DMPT), 2-dimethylamino-ethyl-methacrylate (DMAEMA), and 1-allyl-2-thiourea (ATU) were examined using the bioluminescent bacterial genotoxicity test. 4-Nitroquinoline-N-oxide (4NQO) was prepared for comparison with these chemicals. Acetone solutions of the five polymerization initiators and 4NQO were prepared.

Benzoyl peroxide (BPO), dimethyl-para-toluidine (DMPT), and 1-allyl-2-thiourea (ATU) showed significant genotoxic activity at 24 h in the bioluminescent bacterial genotoxicity test, at concentrations of approximately 5 μM, 4 mM, and 1 mM, respectively. 2-Dimethyloamino-ethyl-methacrylate (DMAEMA) did not have genotoxic activity and CQ had questionable genotoxic activity. In comparison, 4NQO had strong genotoxicity, at 4 μM, roughly the same as that of BPO. Therefore, BPO should be used carefully in clinical dentistry.


Polymerization Peroxide Acetone Visible Light Benzoyl 
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Copyright information

© Springer Science + Business Media, Inc. 2006

Authors and Affiliations

  • Y. Nomura
    • 1
  • W. Teshima
    • 2
  • T. Kawahara
    • 3
  • N. Tanaka
    • 3
  • H. Ishibashi
    • 4
  • M. Okazaki
    • 1
  • K. Arizono
    • 5
  1. 1.Department of Biomaterials ScienceGraduate School of Biomedical Sciences, Hiroshima UniversityHiroshimaJapan
  2. 2.Special Dental Clinic Section, Dental HospitalHiroshima UniversityHiroshimaJapan
  3. 3.Department of Operative Dentistry, Graduate School of Biomedical SciencesHiroshima UniversityHiroshimaJapan
  4. 4.Graduate School of Science and TechnologyNagasaki UniversityNagasakiJapan
  5. 5.Faculty of Environmental and Symbiotic SciencesPrefectural University of KumamotoKumamotoJapan

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