Journal of Materials Science: Materials in Medicine

, Volume 18, Issue 11, pp 2237–2241 | Cite as

Regulation of bone-related genes expression by bone-like apatite in MC3T3-E1 cells

  • Y. F. Tan
  • S. F. Hong
  • X. L. Wang
  • J. Lu
  • H. Wang
  • X. D. Zhang


Bone-like apatite on HA/TCP ceramics sintered at 1,100 °C (HT1) and 1,200 °C (HT2) could be obtained via immersing substrates into simulated body fluid (SBF) for 3 days. When MC3T3-E1 preosteoblastic cells cultured on the surface of the bone-like apatite for 3 days, SEM observations revealed cell membrane features with secreted crystals very similar to in vivo bone formation during intramembranous ossification with a direct bone apposition on the ceramics. According to semi-quantitative RT-PCR method, mRNA expressions of osteocalcin (marker of late-stage differentiation) and type 1 collagen were increased in cultures with HT1S and HT2S when compared to HT1 and HT2 after cultured for 6 days. The results indicated that bone-like apatite had the ability to support the growth of osteoblast-like cells in vitro and to promote osteoblast differentiation by stimulating the expression of major phenotypic markers. Taken together, our findings will be helpful in understanding the mechanism of osteoinductivity of calcium phosphate ceramics and in constructing more appropriate biomimetic substrate.


Apatite Osteocalcin Simulated Body Fluid Apatite Layer Calcium Phosphate Ceramic 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Y. F. Tan
    • 1
  • S. F. Hong
    • 1
  • X. L. Wang
    • 1
  • J. Lu
    • 1
  • H. Wang
    • 2
  • X. D. Zhang
    • 1
  1. 1.Engineering Research Center in BiomaterialsSichuan UniversityChengduChina
  2. 2.Analytical and Testing CenterSichuan UniversityChengduChina

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