Journal of Materials Science: Materials in Medicine

, Volume 16, Issue 12, pp 1099–1104 | Cite as

Nano- and micro-fiber combined scaffolds: A new architecture for bone tissue engineering

  • K. Tuzlakoglu
  • N. Bolgen
  • A. J. Salgado
  • M. E. Gomes
  • E. Piskin
  • R. L. Reis


One possible interesting way of designing a scaffold for bone tissue engineering is to base it on trying to mimic the biophysical structure of natural extracellular matrix (ECM). This work was developed in order to produce scaffolds for supporting bone cells. Nano and micro fiber combined scaffolds were originally produced from starch based biomaterials by means of a fiber bonding and a electrospinning, two step methodology. The cell culture studies with SaOs-2 human osteoblast-like cell line and rat bone marrow stromal cells demonstrated that presence of nanofibers influenced cell shape and cytoskeletal organization of the cells on the nano/micro combined scaffolds. Moreover, cell viability and Alkaline Phosphatase (ALP) activity for both cell types was found to be higher in nano/micro combined scaffolds than in control scaffolds based on fiber meshes without nanofibers.

Consequently, the developed structures are believed have a great potential on the 3D organization and guidance of cells that is provided for engineering of 3-dimensional bone tissues.


Bone Marrow Stromal Cell Bone Tissue Engineering Cell Culture Study Cytoskeletal Organization Step Methodology 


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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • K. Tuzlakoglu
    • 1
    • 2
    • 3
  • N. Bolgen
    • 3
  • A. J. Salgado
    • 1
    • 2
  • M. E. Gomes
    • 1
    • 2
  • E. Piskin
    • 3
  • R. L. Reis
    • 1
    • 2
  1. 1.3B's Research Group—Biomaterials, Biodegradables and BiomimeticsUniv. MinhoBragaPortugal
  2. 2.Department of Polymer EngineeringUniversity of MinhoPortugal
  3. 3.Chem. Eng. Dept. and Bioeng. Div. and TÜBİTAK-Biyomedtek: Center for Biomedical Technologies, BeytepeHacettepe Univ.AnkaraTurkey

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