Nano- and micro-fiber combined scaffolds: A new architecture for bone tissue engineering
- 1.3k Downloads
One possible interesting way of designing a scaffold for bone tissue engineering is to base it on trying to mimic the biophysical structure of natural extracellular matrix (ECM). This work was developed in order to produce scaffolds for supporting bone cells. Nano and micro fiber combined scaffolds were originally produced from starch based biomaterials by means of a fiber bonding and a electrospinning, two step methodology. The cell culture studies with SaOs-2 human osteoblast-like cell line and rat bone marrow stromal cells demonstrated that presence of nanofibers influenced cell shape and cytoskeletal organization of the cells on the nano/micro combined scaffolds. Moreover, cell viability and Alkaline Phosphatase (ALP) activity for both cell types was found to be higher in nano/micro combined scaffolds than in control scaffolds based on fiber meshes without nanofibers.
Consequently, the developed structures are believed have a great potential on the 3D organization and guidance of cells that is provided for engineering of 3-dimensional bone tissues.
KeywordsBone Marrow Stromal Cell Bone Tissue Engineering Cell Culture Study Cytoskeletal Organization Step Methodology
Unable to display preview. Download preview PDF.
- 2.X. LIU and P. X. MA, Annals of Biomedical Eng. 32 (2004) 477.Google Scholar
- 4.Z. M. HUANG, Y. Z. ZHANG, M. KOTAKI and S. RAMAKRISHNA, Composites Sci. and Technology 63 (2003) 2223Google Scholar
- 5.D. LI and Y. XIA, Advanced Mater. 16 (2004) 1151.Google Scholar
- 11.W. LI, C. T. LAURENCIN, E. J. CATERSON, R. S. TUAN and F. K. KO, J. Biomed. Mater. Res. 60 (2002) 613.Google Scholar
- 12.F. YANG, R. MURUGAN, S. WANG and S. RAMAKRISHNA, Biomaterials 26 (2005) 2603.Google Scholar
- 14.W. LI, K. G. DANIELSON, P. G. ALEXANDER and R. S. TUAN, J. Biomed. Mater. Res. Part A 67A (2003) 1105.Google Scholar
- 16.W. LI, R. TULI, X. HUANG, P. LAQUERRIERE and R. S. TUAN, ibid. 26 (2005) 5158.Google Scholar
- 18.M. E. GOMES, R. L. REIS and A. M. CUNHA, Mater. Sci. Eng. C: Biomimet. Supramolec. Syst. 20 (2002) 19.Google Scholar
- 20.F. ROSSO, A. GIORDANO, M. BARBARISI and A. BARBARISI, J. Cellul. Phys. 199 (2004) 174.Google Scholar
- 23.H. HARRIS, Clinica Chimica Acta 186 (1989) 133.Google Scholar
- 24.T. STIGBRAND “Present Status and Future Trends of Human Alkaline Phosphatases. Human Alkaline Phosphatases.” (Alan R. Liss, New York, 1984) p.3.Google Scholar