Development of a system to adsorb drugs onto calcium phosphate materials



Several studies were carried out in order to reduce the systemic use of antibiotics due to the high concentration required to provide the minimum inhibitory concentration (MIC) at infected sites. The aim of this study was to develop a system of drug adsorption onto commercial hydroxyapatite (HA, Ca10(PO4)6(OH)2) and glass reinforced hydroxyapatite (GR-HA) granules. The drug will then be released for the local treatment of periodontitis. The antibiotics used in this study were metronidazole, a specific antibiotic indicated for the systemic treatment of periodontitis, and ampicillin, a wide spectrum antibiotic. UV spectroscopy was used to measure the amount of drug adsorbed onto HA and GR-HA granules. Results showed that metronidazole was unable to adsorb on the material’s surface, as opposed to ampicillin which adsorbed both onto HA and GR-HA. Preliminary release kinetics studies were carried out using a flow through dissolution system. Results are discussed in terms of the influence of the different surface characteristics of the materials on the adsorption processes.


Minimum Inhibitory Concentration Ampicillin Hydroxyapatite Metronidazole Adsorption Process 
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  1. 1.
    R. A. SEYMOUR, P. A. HEASMAN and I. D. M. MACGREGOR, in “Drugs, Diseases, and the Periodontium,” edited by R. A. Seymour and P. A. Heasman (Oxford University Press, Oxford, 1992) p. 1.Google Scholar
  2. 2.
    R. A. CARNEVALE, G. W. GRECO, C. M. BULLARD and J. S. HANKER, in “Biomedical Materials and Devices,” edited by J. S. Hanker and B. L. Giammara (Materials Research Society, Pittsburgh, 1987) p. 247.Google Scholar
  3. 3.
    M. FRIEDMAN and D. STEINBERG, Pharm. Res. 7 (1990) 313.CrossRefGoogle Scholar
  4. 4.
    S. HAGIWARA, N. TAKAMATSU, Y. TOMINAGA and M. UMEDA, J. Period. 69 (1998) 62.Google Scholar
  5. 5.
    T. L. WRIGHT, R. P. ELLEN, J. M. LACROIX, S. SINNADURAI and M. W. MITTELMAN, J. Period. Res. 32 (1997) 473.Google Scholar
  6. 6.
    G. GOLOMB, M. FRIEDMAN, A. SOSKOLNE, A. STABHOLZ and M. N SELA, J. Dent. Res. 63 (1984) 1149.PubMedGoogle Scholar
  7. 7.
    J. M. GOODSON, P. E. HOGAN and S. L. DUNHAM, J. Period. 55 (1984) 81.Google Scholar
  8. 8.
    B. V. SOMAYAJI, U. JARIWALA, P. JAYACHANDRAN, K. VIDYALAKSHIM and R. V. DUDHANI, ibid. 69 (1998) 409.Google Scholar
  9. 9.
    K. STOLTZE, J. Clin. Period. 19 (1992) 698.Google Scholar
  10. 10.
    J. M. GOODSON, D. HOLBOROW, R. L. DUNN, P. HOGAN and S. DUNHAM, J. Period. 54 (1983) 575.Google Scholar
  11. 11.
    D. YU, J. WONG, Y. MATSUDA, J. L. FOX, W. I. HIGUCHI and M. OTSUKA, J. Pharm. Sci. 81 (1992) 529.PubMedGoogle Scholar
  12. 12.
    K. NISHIHARA, Clin. Mater. 12 (1993) 159.CrossRefPubMedGoogle Scholar
  13. 13.
    M. P. FERRAZ, F. J. MONTEIRO and J. D. SANTOS, J. Biomed. Mat. Res. 45 (1999) 376.CrossRefGoogle Scholar
  14. 14.
    M. P. FERRAZ, J. C. KNOWLES, I. OLSEN, F. J. MONTEIRO and J. D. SANTOS, ibid. 47 (1999) 603.CrossRefGoogle Scholar
  15. 15.
    M. A. LOPES, J. D. SANTOS, F. J. MONTEIRO, C. OHTSUKI, A. OSAKA, S. KANEKO and H. INOUE, ibid. 54 (2001) 463.CrossRefGoogle Scholar
  16. 16.
    A. C. QUEIROZ, J. D. SANTOS, F. J. MONTEIRO, I. R. GIBSON and J. C. KNOWLES, Biomaterials 22 (2001) 1393.CrossRefPubMedGoogle Scholar
  17. 17.
    J. D. SANTOS, J. C. KNOWLES, R. L. REIS, F. J. MONTEIRO and G. W. HASTINGS, ibid. 15 (1994) 5.CrossRefPubMedGoogle Scholar
  18. 18.
    J. C. KNOWLES, J. D. SANTOS and G. W. HASTINGS, World Patent, WO0068164 (1994).Google Scholar
  19. 19.
    H. M. MYERS, H. J. TOCHON-DANGUY and C. A. BAUD, Calcif. Tissue Int. 35 (1983) 745.PubMedGoogle Scholar
  20. 20.
    A. RATIER, I. R. GIBSON, S. M. BEST, M. FRECHE, J. L. LACOUT and F. RODRIGUES, Biomaterials 22 (2001) 897.CrossRefPubMedGoogle Scholar
  21. 21.
    D. J. ANDO, in “Ultraviolet and Visible Spectroscopy” (John Wiley & Sons, West Sussex, 1996) p. 16.Google Scholar

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • A. C. Queiroz
    • 1
    • 2
    • 3
  • J. D. Santos
    • 1
    • 2
  • F. J. Monteiro
    • 1
    • 2
  1. 1.INEB—Instituto de Engenharia Biomédica, Laboratório de BiomateriaisPortoPortugal
  2. 2.Faculdade de Engenharia da Universidade do Porto, Departamento de Engenharia Metalúrgica e de MateriaisPortoPortugal
  3. 3.Escola Superior de Tecnologia e Gestão, Ap 574Viana do Castelo CodexPortugal

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