We developed a Polyvinyl butyral (PVB)-zirconium alkoxide hybrid hollow tube by air-gap spinning. Enzyme (β-galactosidase and lipase) was physically entrap-immobilized and disseminated throughout the hollow tube architecture. The enzyme immobilized PVB-Zr hybrid tube shown its stability in phosphate buffer solution, electrolyte solution, and other organic solvents. The apparent Michaelis constant Km for immobilized β-galactosidase (IβG) was 0.079 mol/l compared to 0.067 mol/l for free enzyme. The maximum velocity Vmax was 4.9 μmol.min−1, whereas it was 11.5 μmol.min−1 for free enzyme. The preserved activity of IβG was found to be 90%, after ten reaction cycles. Moreover, up to 70% conversion for citronellyl acetate after 50 h. was achieved by immobilized lipase (IL) in hexane solution. The activity of IL was found to be 62% after 8 repeated uses where free enzyme retained 15% activity. Overall, it was considered that the enzyme activity employed both in the interior and the vicinity of the hollow tube surface.
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A H Bhuiyan thanks the Japanese government for providing PhD financial grant (MEXT: MONBUKAGAKUSHO scholarship). The authors would also thankful to Mr. Tsuyoshi Aoike for his valuable support to perform some experiments.
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Bhuiyan, A.H., Nagakawa, T., Zakaria, M. et al. Utilization of polyvinyl butyral-zirconium alkoxide hybrid hollow tube as an enzyme immobilization carrier. J Mater Sci 56, 8668–8678 (2021). https://doi.org/10.1007/s10853-021-05829-x