Utilization of polyvinyl butyral-zirconium alkoxide hybrid hollow tube as an enzyme immobilization carrier

Abstract

We developed a Polyvinyl butyral (PVB)-zirconium alkoxide hybrid hollow tube by air-gap spinning. Enzyme (β-galactosidase and lipase) was physically entrap-immobilized and disseminated throughout the hollow tube architecture. The enzyme immobilized PVB-Zr hybrid tube shown its stability in phosphate buffer solution, electrolyte solution, and other organic solvents. The apparent Michaelis constant Km for immobilized β-galactosidase (IβG) was 0.079 mol/l compared to 0.067 mol/l for free enzyme. The maximum velocity Vmax was 4.9 μmol.min−1, whereas it was 11.5 μmol.min−1 for free enzyme. The preserved activity of IβG was found to be 90%, after ten reaction cycles. Moreover, up to 70% conversion for citronellyl acetate after 50 h. was achieved by immobilized lipase (IL) in hexane solution. The activity of IL was found to be 62% after 8 repeated uses where free enzyme retained 15% activity. Overall, it was considered that the enzyme activity employed both in the interior and the vicinity of the hollow tube surface.

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Acknowledgements

A H Bhuiyan thanks the Japanese government for providing PhD financial grant (MEXT: MONBUKAGAKUSHO scholarship). The authors would also thankful to Mr. Tsuyoshi Aoike for his valuable support to perform some experiments.

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A. H. Bhuiyan settled the experimental design, performed the experiment, analyzed and measured the results, and prepared the manuscript. T. Nagakawa collected the materials and experimented. M. Zakaria supported in some apparatus settings. K. Nakane provided concept, supervised the work, and revised the manuscript. All authors have read and agreed to manuscript submission.

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The author declares that they have no conflict of interest.

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Correspondence to Koji Nakane.

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Bhuiyan, A.H., Nagakawa, T., Zakaria, M. et al. Utilization of polyvinyl butyral-zirconium alkoxide hybrid hollow tube as an enzyme immobilization carrier. J Mater Sci 56, 8668–8678 (2021). https://doi.org/10.1007/s10853-021-05829-x

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