In situ fabrication of anatase thin films with high percentage of exposed {001} facets to improve biocompatibility with MC3T3-E1 cells

Abstract

In situ anatase thin film with high percentage exposure of {001} facet was fabricated on titanium substrate via one-step hydrothermal method at low temperature from the precursor solution of (NH4)2TiF6 and HF. Nano-inverted bipyramid arrays densely covered the whole surface on as-treated Ti substrate. The structural analysis clearly indicated that as-deposited thin film only consisted of anatase TiO2 single crystal with preferred oriented {001} facets. According to the XRD patterns of as-treated anatase thin film, the integral intensity ratio of (004) to (101) peaks was as high as 7.5 which was remarkably higher than the standard value 0.18. Based on the experimental results, the growth pattern and bonding strength of nano-structural array with high percentage exposure of anatase {001} facet on Ti substrate were discussed. The biocompatibility results demonstrated that MC3T3-E1 cells grown on the as-treated Ti sheet exhibited positive effect on the cell proliferation and significantly high gene expression of ALP, COL-1, BMP-2, OPN and RUNX2 due to its specific property of the surface. Hence, the in situ anatase thin film with high percentage exposure of {001} facet was expected to achieve satisfactory primary stability for osseointegration.

Graphic abstract

In situ fabrication of anatase TiO2 nano-inverted bipyramid arrays with high percentage exposure of {001} facet

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References

  1. 1

    Zhang LC, Chen LY, Wang LQ (2020) Surface modification of titanium and titanium alloys: technologies, developments, and future interests. Adv Eng Mater. https://doi.org/10.1002/adem.201901258

    Article  Google Scholar 

  2. 2

    Kaur M, Singh K (2019) Review on titanium and titanium based alloys as biomaterials for orthopedic applications. Mat Sci Eng C-Mater 102:844–862. https://doi.org/10.1016/j.msec.2019.04.064

    CAS  Article  Google Scholar 

  3. 3

    Li Q, Ma GH, Li JJ, Niinomi M, Nakai M, Koizumi Y, Wei DX, Kakeshita T, Nakano T, Chiba A (2019) Development of low-Young's modulus Ti-Nb-based alloys with Cr addition. J Mater Sci 54:8675–8683. https://doi.org/10.1007/s10853-019-03457-0

    CAS  Article  Google Scholar 

  4. 4

    Liao SC, Chang CT, Chen CY, Lee CH, Lin WL (2020) Functionalization of pure titanium MAO coatings by surface modifications for biomedical applications. Surf Coat Tech. https://doi.org/10.1016/j.surfcoat.2020.125812

    Article  Google Scholar 

  5. 5

    Dikici T, Demirci S, Erol M (2017) Enhanced photocatalytic activity of micro/nano textured TiO2 surfaces prepared by sandblasting/acid-etching/anodizing process. J Alloys Compd 694:246–252. https://doi.org/10.1016/j.jallcom.2016.09.330

    CAS  Article  Google Scholar 

  6. 6

    Liu XZ, Wen K, Deng CM, Yang K, Deng CG, Liu M, Zhou KS (2018) Nanostructured photocatalytic TiO2 coating deposited by suspension plasma spraying with different injection positions. J Therm Spray Technol 27:245–254. https://doi.org/10.1007/s11666-018-0693-3

    CAS  Article  Google Scholar 

  7. 7

    Xiao F, Jiang GQ, Chen CY, Jiang ZL, Liu XZ, Osaka A, Ma XC (2018) Apatite-forming ability of hydrothermally deposited rutile nano-structural arrays with exposed 101 facets on Ti foil. J Mater Sci 53:285–294. https://doi.org/10.1007/s10853-017-1513-8

    CAS  Article  Google Scholar 

  8. 8

    Posternak M, Baldereschi A, Delley B (2019) Adsorption of HPDX and CaHPOx (x=1, …,4) molecules on anatase TiO2 (001) surfaces. Surf Sci 679:93–98. https://doi.org/10.1016/j.susc.2018.09.002

    CAS  Article  Google Scholar 

  9. 9

    Diebold U (2003) The surface science of titanium dioxide. Surf Sci Rep 48:53–229. https://doi.org/10.1016/S0167-5729(02)00100-0

    CAS  Article  Google Scholar 

  10. 10

    Tao T, Bae IT, Woodruff KB, Sauer K, Cho J (2019) Hydrothermally-grown nanostructured anatase TiO2 coatings tailored for photocatalytic and antibacterial properties. Ceram Int 45:23216–23224. https://doi.org/10.1016/j.ceramint.2019.08.017

    CAS  Article  Google Scholar 

  11. 11

    Tong HF, Zhou YY, Chang G, Li P, Zhu RZ, He YB (2018) Anatase TiO2 single crystals with dominant 001 facets: synthesis, shape-control mechanism and photocatalytic activity. Appl Surf Sci 444:267–275. https://doi.org/10.1016/j.apsusc.2018.03.069

    CAS  Article  Google Scholar 

  12. 12

    Maitani MM, Tateyama A, Boix PP, Han GF, Nitta A, Ohtani B, Mathews N, Wada Y (2019) Effects of energetics with 001 facet-dominant anatase TiO2 scaffold on electron transport in CH3NH3PbI3 perovskite solar cells. Electrochim Acta 300:445–454. https://doi.org/10.1016/j.electacta.2019.01.102

    CAS  Article  Google Scholar 

  13. 13

    Yang HG, Sun CH, Qiao SZ, Zou J, Liu G, Smith SC, Cheng HM, Lu GQ (2008) Anatase TiO2 single crystals with a large percentage of reactive facets. Nature 453:638–642. https://doi.org/10.1038/nature06964

    CAS  Article  Google Scholar 

  14. 14

    Sowmiya M, Senthilkumar K (2016) Adsorption of proline, hydroxyproline and glycine on anatase (001) surface: a first-principle study. Theor Chem Acc 135:12. https://doi.org/10.1007/s00214-015-1783-7

    CAS  Article  Google Scholar 

  15. 15

    Bellarditaa M, Garlisib C, Ozer LY, Veneziae AM, Sá J, Mamedov F, Palmisanoa L, Palmisano G (2020) Highly stable defective TiO2-x with tuned exposed facets induced by fluorine: Impact of surface and bulk properties on selective UV/visible alcohol photo-oxidation. Appl Surf Sci. https://doi.org/10.1016/j.apsusc.2020.145419

    Article  Google Scholar 

  16. 16

    He G, Xie L, Yin GF, Liao XM, Zou YW, Huang ZB, Yao YD, Chen XC, Wang FH (2013) Synthesis and mechanism of (101)-preferred orientation rutile titania via anodic spark oxidation. Surf Coat Technol 228:201–208. https://doi.org/10.1016/j.surfcoat.2013.04.030

    CAS  Article  Google Scholar 

  17. 17

    Deki S, Aoi Y, Asaoka Y, Kajinami A, Mizuhata M (1997) Monitoring the growth of titanium oxide thin films by the liquid-phase deposition method with a quartz crystal microbalance. J Mater Chem 7:733–736. https://doi.org/10.1039/a607466i

    CAS  Article  Google Scholar 

  18. 18

    Wu JM, Hayakawa S, Tsuru K, Osaka A (2004) Low-temperature preparation of anatase and rutile layers on titanium substrates and their ability to induce in vitro apatite deposition. J Am Ceram Soc 87:1635–1642. https://doi.org/10.1111/j.1551-2916.2004.01635.x

    CAS  Article  Google Scholar 

  19. 19

    Shou GH, Dong LQ, Liu ZG, Cheng K, Weng WJ (2019) facet-specific mineralization behavior of nano-CaP on anatase polyhedral microcrystals. ACS Biomater Sci Eng 3:875–881. https://doi.org/10.1021/acsbiomaterials.7b00234

    CAS  Article  Google Scholar 

  20. 20

    Puleo DA (1999) Release and retention of biomolecules in collagen deposited on orthopedic biomaterials. Artif Cells Blood Substit Immobil Biotechnol 27:65–75. https://doi.org/10.3109/10731199909117484

    CAS  Article  Google Scholar 

  21. 21

    Xiao SJ, Textor M, Spencer ND, Wieland M, Keller B, Siqrist H (1997) Immobilization of the cell-adhesive Peptide Arg-Gly-Asp-Cys (RGDC) on Titanium surfaces by covalent chemical attachment. J Mater Sci Mater M 8:867–872. https://doi.org/10.1023/A:1018501804943

    CAS  Article  Google Scholar 

  22. 22

    Mikulec LJ, Puleo DA (1996) Use of P-nitrophenyl chloroformate chemistry to immobilize protein on orthopedic biomaterials. J Biomed Mater Res 32:203–208. https://doi.org/10.1002/(SICI)1097-4636(199610)32:2<203:AID-JBM8>3.0.CO;2-X

    CAS  Article  Google Scholar 

  23. 23

    Hayakawa T, Yoshinari M, Nemoto K (2003) Direct attachment of fibronectin to tresyl chloride-activated titanium. J Biomed Mater Res Part A 67A:684–688. https://doi.org/10.1002/jbm.a.10143

    CAS  Article  Google Scholar 

  24. 24

    Welsh WR, Kim HD, Jong YS, Valentini RF (1995) Controlled-release of platelet-derived growth factor using ethylene vinyl acetate copolymer (EVAC) coated on stainless-steel wires. Biomaterials 16:1319–1325. https://doi.org/10.1016/0142-9612(95)91047-3

    Article  Google Scholar 

  25. 25

    de Oliveira RT, Nanci A (2004) Nanotexturing of titanium-based surfaces upregulates expression of bone sialoprotein and osteopontin by cultured osteogenic cells. Biomaterials 25:403–413. https://doi.org/10.1016/S0142-9612(03)00539-8

    CAS  Article  Google Scholar 

  26. 26

    Pang SM, Sun MM, Huang ZQ, He Y, Luo XS, Guo ZZ, Li H (2019) Bioadaptive nanorod array topography of hydroxyapatite and TiO2 on Ti substrate to preosteoblast cell behaviors. J Biomed Mater Res A 107:2272–2281. https://doi.org/10.1002/jbm.a.36735

    CAS  Article  Google Scholar 

  27. 27

    Monjo M, Lamolle SF, Lyngstadaas SP, Ronold HJ, Ellingsen JE (2008) In Vivo Expression of osteogenic markers and bone mineral density at the surface of fluoride-modified titanium implants. Biomaterials 29:3771–3780. https://doi.org/10.1016/j.biomaterials.2008.06.001

    CAS  Article  Google Scholar 

  28. 28

    Dong L, Luo Q, Cheng K, Shi H, Wang Q, Weng WJ, Han WQ (2015) Facet-specific assembly of proteins on SrTiO3 polyhedral nanocrystals. Sci Rep 4:5084. https://doi.org/10.1038/srep05084

    CAS  Article  Google Scholar 

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Acknowledgements

This work was supported by the Natural Science Foundation of Zhejiang Province, China (grant number LY15E020010, LGF18H140006), and National Natural Science Foundation of China (grant number 50702050).

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Correspondence to Xiao-chun Ma.

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Xiao, F., Xiang, J., Cheng, G. et al. In situ fabrication of anatase thin films with high percentage of exposed {001} facets to improve biocompatibility with MC3T3-E1 cells. J Mater Sci (2020). https://doi.org/10.1007/s10853-020-04957-0

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