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Silver nanoparticles-doped collagen–alginate antimicrobial biocomposite as potential wound dressing

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Abstract

Development of novel wound dressing with potent antibacterial activity is crucial for wound healing and tissue regeneration. In this work, we aim to prepare silver nanoparticles (AgNPs)-doped collagen–alginate (CA–AgNPs) biocomposite, which may possess antibacterial activity and be used as wound dressing. AgNPs were synthesized using NaBH4 as reducing agent and polyvinyl pyrrolidone as stabilizing agent. The formation of the AgNPs was confirmed by ultraviolet–visible spectrophotometer and transmission electron microscopy. Then, the as-prepared AgNPs were mixed with sodium alginate and collagen to obtain CA–AgNPs biocomposite. The CA–AgNPs biocomposite was fully characterized to verify the presence of AgNPs in the biocomposite. In vitro cytotoxicity assay illustrated that the CA–AgNPs biocomposite possessed negligible cytotoxicity at low AgNPs concentration. Furthermore, the antibacterial activity of the CA–AgNPs biocomposite was assessed against Staphylococcus aureus and Escherichia coli through agar diffusion method. Inhibition zone indicated that CA–AgNPs biocomposite possessed much higher antimicrobial activity than that of CA biocomposite, which strengthened with the increase in the AgNPs contents. Taken together, our finding suggested that the CA–AgNPs biocomposite showed strong potential as wound dressing.

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References

  1. Balasubramani M, Kumar TR, Babu M (2001) Skin substitutes: a review. Burns 27:534–544

    Article  Google Scholar 

  2. You C, Li Q, Wang X et al (2017) Silver nanoparticle loaded collagen/chitosan scaffolds promote wound healing via regulating fibroblast migration and macrophage activation. Sci Rep 7:10489

    Article  Google Scholar 

  3. Ma Y, Xin L, Tan H et al (2017) Chitosan membrane dressings toughened by glycerol to load antibacterial drugs for wound healing. Mat Sci Eng C Mater 81:522–531

    Article  Google Scholar 

  4. Khor E, Lim LY (2003) Implantable applications of chitin and chitosan. Biomaterials 24:2339–2349

    Article  Google Scholar 

  5. Loke WK, Lau SK, Yong LL, Khor E, Sum CK (2000) Wound dressing with sustained anti-microbial capability. J Biomed Mater Res 53:8–17

    Article  Google Scholar 

  6. Jayakumar R, Prabaharan M, Kumar PTS, Nair SV, Tamura H (2011) Biomaterials based on chitin and chitosan in wound dressing applications. Biotechnol Adv 29:322–337

    Article  Google Scholar 

  7. Xie H, Chen X, Shen X et al (2018) Preparation of chitosan–collagen–alginate composite dressing and its promoting effects on wound healing. Int J Biol Macromol 107:93–104

    Article  Google Scholar 

  8. Michalska-Sionkowska M, Walczak M, Sionkowska A (2017) Antimicrobial activity of collagen material with thymol addition for potential application as wound dressing. Polym Test 63:360–366

    Article  Google Scholar 

  9. Pal S, Nisi R, Stoppa M, Licciulli A (2017) Silver-functionalized bacterial cellulose as antibacterial membrane for wound-healing applications. ACS Omega 2:3632–3639

    Article  Google Scholar 

  10. Dowling MB, Chaturvedi A, MacIntire IC et al (2016) Determination of efficacy of a novel alginate dressing in a lethal arterial injury model in swine. Injury 47:2105–2109

    Article  Google Scholar 

  11. Ye J, Shi X, Chen X et al (2014) Chitosan-modified, collagen-based biomimetic nanofibrous membranes as selective cell adhering wound dressings in the treatment of chemically burned corneas. J Mater Chem B 2:4226–4236

    Article  Google Scholar 

  12. Baniasadi M, Minary-Jolandan M (2015) Alginate–collagen fibril composite hydrogel. Materials (Basel) 8:799–814

    Article  Google Scholar 

  13. Lee BR, Hwang JW, Choi YY, Wong SF, Hwang YH, Lee DY, Lee SH (2012) In situ formation and collagen–alginate composite encapsulation of pancreatic islet spheroids. Biomaterials 33:837–845

    Article  Google Scholar 

  14. Lee H-j, Ahn S-H, Kim GH (2011) Three-dimensional collagen/alginate hybrid scaffolds functionalized with a drug delivery system (DDS) for bone tissue regeneration. Chem Mater 24:881–891

    Article  Google Scholar 

  15. Bakare RA, Bhan C, Raghavan D (2014) Synthesis and characterization of collagen grafted poly(hydroxybutyrate-valerate) (PHBV) scaffold for loading of bovine serum albumin capped silver (Ag/BSA) nanoparticles in the potential use of tissue engineering application. Biomacromolecules 15:423–435

    Article  Google Scholar 

  16. GhavamiNejad A, Rajan Unnithan A, Ramachandra Kurup Sasikala A et al (2015) Mussel-inspired electrospun nanofibers functionalized with size-controlled silver nanoparticles for wound dressing application. ACS Appl Mater Interfaces 7:12176–12183

    Article  Google Scholar 

  17. Fei X, Jia M, Du X et al (2013) Green synthesis of silk fibroin–silver nanoparticle composites with effective antibacterial and biofilm-disrupting properties. Biomacromolecules 14:4483–4488

    Article  Google Scholar 

  18. Lee HY, Park HK, Lee YM, Kim K, Park SB (2007) A practical procedure for producing silver nanocoated fabric and its antibacterial evaluation for biomedical applications. Chem Commun 28:2959–2961

    Article  Google Scholar 

  19. Bao H, Yu X, Xu C, Li X, Li Z, Wei D, Liu Y (2015) New toxicity mechanism of silver nanoparticles: promoting apoptosis and inhibiting proliferation. PLoS ONE 10:e0122535

    Article  Google Scholar 

  20. Paladini F, Pollini M, Sannino A, Ambrosio L (2015) Metal-based antibacterial substrates for biomedical applications. Biomacromolecules 16:1873–1885

    Article  Google Scholar 

  21. Gopinath V, Velusamy P (2013) Extracellular biosynthesis of silver nanoparticles using Bacillus sp GP-23 and evaluation of their antifungal activity towards Fusarium oxysporum. Spectrochim Acta A Mol Biomol Spectrosc 106:170–174

    Article  Google Scholar 

  22. Zhao X, Li Q, Ma X, Quan F, Wang J, Xia Y (2015) The preparation of alginate–AgNPs composite fiber with green approach and its antibacterial activity. J Ind Eng Chem 24:188–195

    Article  Google Scholar 

  23. Tsuji M, Gomi S, Maeda Y et al (2012) Rapid transformation from spherical nanoparticles, nanorods, cubes, or bipyramids to triangular prisms of silver with PVP, citrate, and H2O2. Langmuir 28:8845–8861

    Article  Google Scholar 

  24. Sarkar AK, Saha A, Midya L, Banerjee C, Mandre N, Panda AB, Pal S (2017) Cross-linked biopolymer stabilized exfoliated titanate nanosheet-supported AgNPs: a green sustainable ternary nanocomposite hydrogel for catalytic and antimicrobial activity. ACS Sustain Chem Eng 5:1881–1891

    Article  Google Scholar 

  25. Goli KK, Gera N, Liu X, Rao BM, Rojas OJ, Genzer J (2013) Generation and properties of antibacterial coatings based on electrostatic attachment of silver nanoparticles to protein-coated polypropylene fibers. ACS Appl Mater Interfaces 5:5298–5306

    Article  Google Scholar 

  26. Chook SW, Chia CH, Zakaria S, Neoh HM, Jamal R (2017) Effective immobilization of silver nanoparticles on a regenerated cellulose–chitosan composite membrane and its antibacterial activity. New J Chem 41:5061–5065

    Article  Google Scholar 

  27. Goli KK, Gera N, Liu X, Rao BM, Rojas OJ, Genzer J (2013) Generation and properties of antibacterial coatings based on electrostatic attachment of silver nanoparticles to protein-coated polypropylene fibers. ACS Appl Mater Interfaces 5:5298–5306

    Article  Google Scholar 

  28. Tejamaya M, Roemer I, Merrifield RC, Lead JR (2012) Stability of citrate, PVP, and PEG coated silver nanoparticles in ecotoxicology media. Environ Sci Technol 46:7011–7017

    Article  Google Scholar 

  29. Venkatesan J, Lee JY, Kang DS, Anil S, Kim SK, Shim MS, Kim DG (2017) Antimicrobial and anticancer activities of porous chitosan–alginate biosynthesized silver nanoparticles. Int J Biol Macromol 98:515–525

    Article  Google Scholar 

  30. Iqbal B, Muhammad N, Jamal A et al (2017) An application of ionic liquid for preparation of homogeneous collagen and alginate hydrogels for skin dressing. J Mol Liq 243:720–725

    Article  Google Scholar 

  31. Zeng M, Xiao H, Zhang X, Sun X, Qi C, Wang B (2011) A novel chitosan/polyvinyl pyrrolidone (CS/PVP) three-dimensional composite and its mechanism of strength improvement. J Macromol Sci Part B Phys 50:1413–1422

    Article  Google Scholar 

  32. Ahamed MI, Sankar S, Kashif PM, Basha SK, Sastry TP (2015) Evaluation of biomaterial containing regenerated cellulose and chitosan incorporated with silver nanoparticles. Int J Biol Macromol 72:680–686

    Article  Google Scholar 

  33. Lin S, Chen L, Huang L, Cao S, Luo X, Liu K (2015) Novel antimicrobial chitosan–cellulose composite films bioconjugated with silver nanoparticles. Ind Crop Prod 70:395–403

    Article  Google Scholar 

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Acknowledgements

This work was supported by Natural Science Foundation of Jiangsu Province (BK 20170202, BK 20170203) and National Natural Science of China (21574059, 81503007). This work was also supported by national first-class discipline program of Light Industry Technology and Engineering (LITE2018-20).

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  1. Huijie Zhang and Mengxia Peng have contributed equally to this work.

Authors and Affiliations

  1. School of Pharmaceutical Sciences, Jiangnan University, Wuxi, 214122, China

    Huijie Zhang, Mengxia Peng, Peng Zhao, Lipeng Qiu, Juan Zhou & Jinghua Chen

  2. Department of Oncology, Nanjing Medical University Affiliated Wuxi Second Hospital, Wuxi, 214002, China

    Ting Cheng

  3. Department of Burns and Plastic Surgery, The Third Affiliated Hospital with Nantong University, Wuxi, 214041, China

    Peng Zhao & Guozhong Lu

Authors
  1. Huijie Zhang
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  2. Mengxia Peng
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  3. Ting Cheng
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  4. Peng Zhao
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  5. Lipeng Qiu
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  6. Juan Zhou
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  7. Guozhong Lu
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  8. Jinghua Chen
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Correspondence to Guozhong Lu or Jinghua Chen.

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Zhang, H., Peng, M., Cheng, T. et al. Silver nanoparticles-doped collagen–alginate antimicrobial biocomposite as potential wound dressing. J Mater Sci 53, 14944–14952 (2018). https://doi.org/10.1007/s10853-018-2710-9

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  • DOI: https://doi.org/10.1007/s10853-018-2710-9

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