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Unveiling the slow release behavior of hollow particles with prolonged antibacterial activity

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Abstract

The aim of this work was to fabricate polylactide-based hollow microspheres to act as slow-release antimicrobial carriers with prolonged antibacterial activity for controlled release applications and also to sharpen our understanding behind its slow release. Hollow particles were fabricated via water/oil/water (w/o/w) double-emulsion method by using hot water to accelerate solvent evaporation rate in order to make hollow core whereas non-hollow (solid) microspheres were formed via simple oil/water (o/w) emulsion method. Interestingly, in vitro release of hydrophilic antibacterial from solid microspheres (hydrophobic) were extremely slow due to strong association between antibacterial and polymer (polylactide) matrix via H-bonding, investigated by Raman microscopy and DSC study. The release rate increases significantly (> 4 times) in the case of hollow particles. The release kinetics study revealed that both hollow and solid particles predominantly followed diffusion-controlled process rather than a degradation pathway. These hollow microspheres demonstrate great promise as antibacterial agents against both gram-negative and gram-positive bacteria over a period of 30 days at 37 °C. The antibacterial effect of these particles was also evaluated on a food model (watermelon juice) and a significant reduction in bacterial growth was observed especially in the case of hollow microparticles. Hence, PLA-based hollow microspheres can be proposed as slow-release antimicrobial carriers for drug delivery or active packaging applications due to its ease of preparation and simplicity.

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Authors and Affiliations

  1. Centre for Polymer Science and Engineering, Indian Institute of Technology, Delhi, India

    Agni Kumar Biswal, Mohd. Usmani & Sampa Saha

  2. Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi, India

    Shaikh Ziauddin Ahammad

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  1. Agni Kumar Biswal
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Biswal, A.K., Usmani, M., Ahammad, S.Z. et al. Unveiling the slow release behavior of hollow particles with prolonged antibacterial activity. J Mater Sci 53, 5942–5957 (2018). https://doi.org/10.1007/s10853-018-1991-3

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