Superparamagnetic nanohybrids with cross-linked polymers providing higher in vitro stability
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A simple, rapid, reproducible, and scalable method for generating highly stable cross-linked superparamagnetic nanohybrids was developed. Pre-coating of superparamagnetic iron oxide nanoparticle surfaces with a biocompatible polymer, hydroxy polyvinyl alcohol (PVA-OH) prior to cross-linking with silica precursor resulted in improved stability, uniform morphologies and allows for further surface functionalization. The obtained magnetic nanohybrids contain a non-porous silica layer, are monodisperse (size 50.0 ± 3.7 nm), and show colloidal stability applicable for biomedical applications (pH 7.35–7.45) with long shelf life (>9 months). In vitro studies indicate that as-prepared nanohybrids are non-cytotoxic and highly robust toward endosomal/lysosomal conditions, with no particle dissolution evident for up to 42 days. As a demonstration of the potential utility of these nanohybrids in medical diagnostic applications (e.g., MRI), surface functionalization with folic acid resulted in particle recognition and affinity to folate receptor-positive cervix (HeLa) cells. Accordingly, the facile development of these non-toxic, stable cross-linked magnetic nanohybrids, with the added benefit of scalable preparation, should serve as an entry point for the further development of safer, target specific, MRI contrast agents for cancer diagnosis.
KeywordsPrussian Blue Colloidal Stability Silica Layer Silica Coating Cell Index
The authors would like to acknowledge financial support from the National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand, the Swiss National Science Foundation (SNSF), the Laboratory of Powder Technology, Ecole Polytechnique Fédérale de Lausanne (EPFL), and the Faculty of Medicine Siriraj Hospital, Mahidol University.
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Conflict of interest
The authors declare that they have no conflict of interest.
- 1.Mahmoudi M, Hosseinkhani H, Hosseinkhani M, Boutry S, Simchi A, Journeay WS, Subramani K, Laurent S (2011) Magnetic resonance imaging tracking of stem cells in vivo using iron oxide nanoparticles as a tool for the advancement of clinical regenerative medicine. Chem Rev 111:253–280CrossRefGoogle Scholar
- 14.Molday RS (1984) Magnetic iron-dextran microspheres, U.S. Patent 4,452,773Google Scholar
- 23.Schulze F, Dienelt A, Geissler S, Zaslansky P, Schoon J, Henzler K, Guttmann P, Gramoun A, Crowe LA, Maurizi L, Vallée JP, Hofmann H, Duda GN, Ode A (2014) Amino-polyvinyl alcohol coated superparamagnetic iron oxide nanoparticles are suitable for monitoring of human mesenchymal stromal cells in vivo. Small 10:4340–4351Google Scholar
- 38.Sonvico F, Mornet S, Vasseur S, Dubernet C, Jaillard D, Degrouard J, Hoebeke J, Duguet E, Colombo P, Couvreur P (2005) Folate-conjugated iron oxide nanoparticles for solid tumor targeting as potential specific magnetic hyperthermia mediators: synthesis, physicochemical characterization, and in vitro experiments. Bioconjug Chem 16:1181–1188CrossRefGoogle Scholar
- 43.Otero-González L, Sierra-Alvarez R, Boitana S, Field JA (2012) Application and validation of an impedance-based real time cell analyzer to measure the toxicity of nanoparticles impacting human bronchial epithelial cells. Environ Sci Technol 46:10271–10278Google Scholar