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Journal of Materials Science

, Volume 49, Issue 18, pp 6290–6301 | Cite as

Surface ligand influenced free radical protection of superparamagnetic iron oxide nanoparticles (SPIONs) toward H9c2 cardiac cells

  • Faruq Mohammad
  • Nor Azah Yusof
Article

Abstract

There have been a number of studies which deal with either toxic or non-toxic nature of superparamagnetic iron oxide nanoparticles (SPIONs); however, there is no clear cut information about their exact behavior and the reasons for its dual action. The objective of the present study was to investigate the SPIONs having similar oxidation states, but varying surface ligands and their role in terms of protecting the iron-mediated toxic responses. The four different SPIONs includes: (i) SPIONs containing oleic acid (SPIONs-1), (ii) SPIONs without any surface ligand (SPIONs-2), (iii) SPIONs containing cysteamine ligand (SPIONs-3), and (iv) SPIONs having both of oleic acid and cysteamine ligand. The particle size, surface functionality, and electronic oxidation states were confirmed by the HRTEM, FT-IR, and XPS analysis, respectively. On in vitro testing of all four SPIONs with H9c2 cardiomyocyte cell line, the SPIONs-2 without any surface ligand found to exhibit significant decrease in the viability of cells at a concentration of 200 μg mL−1 for 16-h exposure period. Further investigation of toxicity mechanism resulted in the fact that the SPIONs-2 involved in the formation of ROS due to the role played by the more electron deficient Fe3+ form of iron, there by decreased the glutathione release, increased DNA cleavage, and disrupted the mitochondrial transmembrane potential. However, the presence of unsaturation and/or thiol group (–SH) containing ligands on other SPIONs protected the cardiac cells from undergoing ROS-induced oxidative stress. Further, the results of the study confirming the importance of having unsaturated double bonds and/or –SH group possessing ligands onto the surface of SPIONs by means of protecting the cells from the influence of electron deficient Fe3+ state of iron.

Keywords

Oleic Acid Trolox Maghemite H9c2 Cell Cysteamine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

SPIONs

Superparamagnetic iron oxide nanoparticles

NPs

Nanoparticles

HRTEM

High-resolution transmission electron microscopy

FT-IR

Fourier transform infrared spectroscopy

XPS

X-ray photoelectron spectroscopy

ROS

Reactive oxygen species

Notes

Acknowledgements

The authors would like to thank Universiti Putra Malaysia for the support and funding.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Institute of Advanced TechnologyUniversiti Putra MalaysiaSerdangMalaysia

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