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Poly (pyrrole-co-N-methyl pyrrole) for application to cholesterol sensor

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Abstract

Cholesterol oxidase (ChOx) has been electrochemically entrapped onto p-toluene sulphonate (PTS) doped poly (pyrrole-co-N-methyl pyrrole) (1:1) on indium-tin-oxide (ITO) glass plates. This ChOx-copolymer-PTS/ITO bioelectrode has been characterized using cyclic voltammetry (CV), Fourier transform infrared (FTIR) spectroscopy, UV-visible spectroscopy, scanning electron microscopy (SEM) techniques. The cholesterol bioelectrode shows response time of 19 s, linearity from 50 to 400 mg/dL as a function of cholesterol concentration. It exhibits optimum pH range between 6.5 and 7.5, shelf-life of up to 6 weeks at 4 °C and shows almost undisturbed response in presence of interferents like ascorbic acid, uric acid and glucose.

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Acknowledgements

Tinku Basu is thankful to Dr. Ashok K.Chauhan, Founder President of Amity University, U.P., India, for his constant encouragement and motivation to perform the innovative work in the development of Science and Technology. We thank Director, National Physical Laboratory, New Delhi, India, for the facilities. PRS is grateful to the Council of Scientific Industrial Research (CSIR), India, for the award of the Senior Research Associate (SRA).

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Authors and Affiliations

  1. Amity Institute of Engineering and Technology, Amity University, Noida, UP, India

    K. Singh

  2. Amity Institute of Nano Technology, Amity University, Noida, UP, India

    T. Basu

  3. Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr K. S. Krishnan Marg, New Delhi, 110012, India

    Pratima R. Solanki & Bansi Dhar Malhotra

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  1. K. Singh
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  2. T. Basu
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  3. Pratima R. Solanki
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  4. Bansi Dhar Malhotra
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Correspondence to Bansi Dhar Malhotra.

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Singh, K., Basu, T., Solanki, P.R. et al. Poly (pyrrole-co-N-methyl pyrrole) for application to cholesterol sensor. J Mater Sci 44, 954–961 (2009). https://doi.org/10.1007/s10853-008-3184-y

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  • DOI: https://doi.org/10.1007/s10853-008-3184-y

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