Sol–gel derived composite from bioactive glass–polyvinyl alcohol

  • Hermes S. Costa
  • Magda F. Rocha
  • Giovanna I. Andrade
  • Edel F. Barbosa-Stancioli
  • Marivalda M. Pereira
  • Rodrigo L. Orefice
  • Wander L. Vasconcelos
  • Herman S. Mansur


Investigation of novel biomaterials for bone engineering is based on the development of porous scaffolds, which should match the properties of the tissue that is to be replaced. These materials need to be biocompatible, ideally osteoinductive, osteoconductive, and mechanically well-matched. In the present paper, we report the preparation and characterization of hybrid macroporous scaffold of polyvinyl alcohol (PVA)/bioactive glass through the sol–gel route. Hybrids containing PVA (80, 70 and 60 wt%) and bioactive glass with composition 58SiO2–33CaO–9P2O5 were synthesized by foaming a mixture of polymer solution and bioactive glass via sol–gel precursor solution. PVA with two different degree of hydrolysis (DH), 98.5% (high degree) and 80% (low degree) were also investigated, in order to evaluate the influence of residual acetate group present in polymer chain on the final structure and properties of 3D porous composite produced. The microstructure, morphology and crystallinity of the hybrid porous scaffolds were characterized by X-ray diffraction (XRD), Infrared Fourier Transform spectrometry (FTIR) and Scanning electron microscopy (SEM/EDX) analysis. In addition, specific surface area was assessed by B.E.T. nitrogen adsorption method and mechanical behavior was evaluated by compression tests. Preliminary cytotoxicity and cell viability were also performed by the MTT assay. VERO cell monolayers were grown in 96-well microtiter plates. The results have clearly showed that hybrid foams of polyvinyl alcohol/bioactive glass (PVA/BG) with interconnected macroporous 3D structure were successfully produced. All the tested hybrids of PVA/BG have showed adequate cell viability properties for potential biological applications.


Foam Simulated Body Fluid Bioactive Glass Gelation Time Hybrid Scaffold 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors acknowledge National Council for Scientific and Technological Development (CNPq) and State of Minas Gerais Research Foundation (FAPEMIG) for financial support on this project. We are also grateful to Prof. Dr. Dagoberto Santos for the facilities of Electronic Microscopy Laboratory and Dr. Vilma Costa for the kind assistance on sol–gel processing.


  1. 1.
    Kim HM (2003) Curr Opin Solid State Mat Sci 7:289CrossRefGoogle Scholar
  2. 2.
    Nissan BB (2003) Curr Opin Solid State Mat Sci 7:283CrossRefGoogle Scholar
  3. 3.
    Tadic D, Beckmann F, Schwarz K, Epple M (2004) Biomaterials 25:3335CrossRefGoogle Scholar
  4. 4.
    Jones JR, Hench LL (2003) Curr Opin Solid State Mat Sci 7:301CrossRefGoogle Scholar
  5. 5.
    Olivier V, Faucheux N, Hardouin P (2004) Drug Discov Today 9:803CrossRefGoogle Scholar
  6. 6.
    Burg KJL, Porter S, Kellam JF (2000) Biomaterials 21:2347CrossRefGoogle Scholar
  7. 7.
    Rezwan K, Chen QZ, Blaker JJ, Boccaccini AB (2006) Biomaterials 27:3413CrossRefGoogle Scholar
  8. 8.
    Hench LL (1991) J Am Ceram Soc 74:1487CrossRefGoogle Scholar
  9. 9.
    Jones RJ, Ehrenfried LM, Hench LL (2005) Biomaterials 27:964CrossRefGoogle Scholar
  10. 10.
    Pereira MM, Jones JR, Orefice RL, Hench LL (2005) J Mater Sci: Mater Med 16:1045CrossRefGoogle Scholar
  11. 11.
    Chiellini E, Corti A, D’antone S, Solaro R (2003) Prog Polym Sci 28:963CrossRefGoogle Scholar
  12. 12.
    Pereira APV, Wander LV, Orefice RL (2000) J Non-Cryst Solids 273:180CrossRefGoogle Scholar
  13. 13.
    Paul W, Sharma CP (2006) Am J Biochem Biotechnol 2(2):41CrossRefGoogle Scholar
  14. 14.
    Yamaoka T, Tabata Y, Ikada Y (1995) J Pharmaceut Pharmacol 47:479Google Scholar
  15. 15.
    Brinker CJ, Scherer GW (1990) Sol–gel science: the physics and chemistry of sol–gel processing. Academic Press, New York, NYGoogle Scholar
  16. 16.
    Oki A, Qiu X, Alawode O, Foley B (2006) Mater Lett 60:2751CrossRefGoogle Scholar
  17. 17.
    Kokubo T (1998) Acta Mater 46:2519CrossRefGoogle Scholar
  18. 18.
    Pereira MM, Jones JR, Hench LL (2005) Adv Appl Ceram 104(1):35CrossRefGoogle Scholar
  19. 19.
    Mansur HS, Oréfice RL, Mansur AAP (2004) Polymer 45:7193CrossRefGoogle Scholar
  20. 20.
    Andrade G, Barbosa-Stancioli1 EF, Piscitelli Mansur AA, Vasconcelos WL, Mansur HS (2006) Biomed Mater 1:221CrossRefGoogle Scholar
  21. 21.
    Mansur HS, Oréfice RL, Vasconcelos WL, Lobato ZP, Machado LJ (2005) J Mater Sci: Mater Med 16:333CrossRefGoogle Scholar
  22. 22.
    Coates J (2000) In: Meyers (ed) Encyclopedia of analytical chemistry. Wiley, Chichester, pp 10815–10837Google Scholar
  23. 23.
    Almeida RM, Pantano CG (1990) J Appl Phys 68:1225CrossRefGoogle Scholar
  24. 24.
    Roman J, Padilla S, Vallet-Regı M (2003) Sol–gel glasses as precursors of bioactive glass ceramics. Chem Mater 15:798; Pereira MM, Clark AE, Hench LL (1994) J Biomed Mater Res 28:693Google Scholar
  25. 25.
    Gibson LJ (2005) J Biomech 38:377CrossRefGoogle Scholar
  26. 26.
    Shi D, Jiang G (1998) Mater Sci Eng C 6:175–182CrossRefGoogle Scholar
  27. 27.
    Montjovent MO, Mathieu L, Hinz B, Applegate LL, Bourban PE, Zambelli PY, Manson JA, Pioletti DP (2005) Tissue Eng 11:1640CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Hermes S. Costa
    • 1
  • Magda F. Rocha
    • 1
  • Giovanna I. Andrade
    • 2
  • Edel F. Barbosa-Stancioli
    • 2
  • Marivalda M. Pereira
    • 1
  • Rodrigo L. Orefice
    • 1
  • Wander L. Vasconcelos
    • 1
  • Herman S. Mansur
    • 1
  1. 1.Department of Metallurgical and Materials Engineering, Laboratory of Biomaterials and Tissue EngineeringFederal University of Minas GeraisBelo HorizonteBrazil
  2. 2.Department of Microbiology, Institute of Biological SciencesFederal University of Minas GeraisBelo HorizonteBrazil

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