Characterization and application of magnetic P (St-co-MAA-co-AM)/SiO2 composite microspheres
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A sol–gel procedure was used to cover the magnetic P (St-co-MAA-co-AM) microspheres with a SiO2 layer, forming MMS/SiO2 composite micospheres. The composite spheres were synthesized by a two-step process. First, magnetic P (St-co-MAA-co-MA) microspheres (MMS) were prepared by microemulsion polymerization of styrene (St), methacrylic acid (MAA) and acryamide (AM) in the presence of magnetic fluid. In the next step, MMS/SiO2 spheres were obtained by addition of tetraethyl orthsilicate (TEOS) to the mixture of MMS, ammonium hydroxide and ethanol. The morphology, structure and properties of the MMS/SiO2 were characterized and the results indicate that the MMS/SiO2 spheres are about 2 μm in size with superparamagnetic behavior. The surface of the MMS/SiO2 spheres was functioned with –COOH successfully. Bioconjugation to IgG was also studied.
KeywordsAmmonium Hydroxide Vibrate Sample Magnetometer Magnetic Fluid Methacrylic Acid Tetraethyl Orthosilicate
This work is supported by the National Nature Science Foundation of China under Grant nos. 10574047 and 10574048 and Doctor Foundation of China (No. 20030487003). This work was also supported by the National 973 Project under Grant. No. 2006CB921606.