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Characterisation and anti-inflammatory evaluation of the inclusion complex of ellagic acid with hydroxypropyl-β-cyclodextrin

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Abstract

The aim of the present work was to examine ellagic acid hydroxypropyl-β-cyclodextrin complex (EA-HP-β-CD) obtained through the freeze-drying method via FTIR, XRD, SEM, NMR and molecular modeling, as well as to investigate it’s antioxidant and anti-inflammatory activity on carrageenan-induced paw oedema in rats. Phase-solubility study showed that ellagic acid (EA) formed 1:2 stoichiometric inclusion complex with hydroxypropyl-β-cyclodextrin (HP-β-CD). The XRD and SEM analysis were confirmed true inclusion complex formation of EA with HP-β-CD by freeze-drying method when compared with a physical mixture. The FTIR, NMR and molecular modeling studies suggested that the carbonyl groups and hydroxyl groups of EA were involved in the inclusion complexation with HP-β-CD. EA-HP-β-CD exhibited better protection from protein denaturation and lysis of erythrocyte membrane as compared to positive controls. In vivo anti-inflammatory evaluation of EA-HP-β-CD in rats showed significant anti-inflammatory and antioxidant activity on carrageenan-induced rat paw oedema. The present findings suggest that EA-HP-β-CD inclusion complex enhances the anti-inflammatory and antioxidant effect of EA in experimental animal models.

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Acknowledgements

Authors are thankful to the University Grant Commission, India (UGC) for the research fellowship awarded under special assistance programme (SAP) and Sophisticated Analytical Instrument Facility, Indian Institute of Technology, Madras, India, for spectral analysis.

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The authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Pharmacology Research Lab 1, Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai, 400019, India

    Vipin D. Bulani, Pankaj S. Kothavade, Dnyaneshwar M. Nagmoti & Archana R. Juvekar

  2. Pharmaceutical Chemistry Lab 2, Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Matunga, Mumbai, 400019, India

    Harish S. Kundaikar & Mariam S. Degani

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  1. Vipin D. Bulani
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Correspondence to Archana R. Juvekar.

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Bulani, V.D., Kothavade, P.S., Nagmoti, D.M. et al. Characterisation and anti-inflammatory evaluation of the inclusion complex of ellagic acid with hydroxypropyl-β-cyclodextrin. J Incl Phenom Macrocycl Chem 82, 361–372 (2015). https://doi.org/10.1007/s10847-015-0498-7

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