Voltammetric studies of the interaction of 6-mercaptopurine with cucurbit[7]uril and DNA

  • Ying Huang
  • Sai-Feng Xue
  • Zhu Tao
  • Qian-Jiang Zhu
  • Yan-Hui Ma
  • Si-Huang Zhong
Original Article


The interaction of 6-mercaptopurine (6-MP), an antitumor drug, with cucurbit[7]uril (Q[7]) and DNA in an acetate buffer solution was studied by differential pulse voltammetry (DPV) and cyclic voltammetry(CV). The electrochemical data indicated a 1:1 complex formation of 6-MP with Q[7] and DNA. The formation constants of these complexes were determined based on the variations in the current. Moreover, the interactions of the 6-MP-Q[7] inclusion complex with DNA have been investigated by means of voltammetry. The results suggested that 6-MP displayed a high affinity for Q[7] and that the inclusion complex did not decompose when it bound to DNA. It can be inferred from the experimental data that the binding model of 6-MP to DNA may be ‘electrostatic binding’. In addition, the formation of inclusion complexes between Q[7] and 6-MP was confirmed by UV-Vis spectroscopy and the 1H NMR technique.


Voltammetry 6-mercaptopurine Cucurbit[7]uril DNA 



We acknowledge the support of the National Natural Science Foundation of China (Grant No. 20972034), Natural Science fund of Science and Technology Department of GuiZhou Province(Grant No. J-2009-2288), Introduced Talents Start-up Project of GuiZhou University(Grant No.2009023), and the Foundation of the Governor of Guizhou Province.


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Ying Huang
    • 1
    • 2
  • Sai-Feng Xue
    • 1
  • Zhu Tao
    • 1
  • Qian-Jiang Zhu
    • 1
  • Yan-Hui Ma
    • 1
  • Si-Huang Zhong
    • 1
  1. 1.Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou ProvinceGuizhou UniversityGuiyangPeople’s Republic of China
  2. 2.The Engineering and Research Center for Southwest Bio-Pharmaceutical resources of National Education Ministry of ChinaGuizhou UniversityGuiyangPeople’s Republic of China

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