Host–guest system of 4-nerolidylcatechol in 2-hydroxypropyl-β-cyclodextrin: preparation, characterization and molecular modeling

  • Líllian Amélia Soares
  • Ana Flávia Vasconcelos Borges Leal
  • Leonardo Fernandes Fraceto
  • Elaine Rose Maia
  • Inês Sabioni Resck
  • Massuo Jorge Kato
  • Eric de Sousa Gil
  • Aparecido Ribeiro de Sousa
  • Luiz Carlos da Cunha
  • Kênnia Rocha Rezende
Original Article


The interaction of 4-nerolidylcatechol (4-NRC), a potent antioxidant agent, and 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) was investigated by the solubility method using Fourier transform infrared (FTIR) methods in addition to UV–Vis, 1H-nuclear magnetic resonance (NMR) spectroscopy and molecular modeling. The inclusion complexes were prepared using grinding, kneading and freeze-drying methods. According to phase solubility studies in water a BS-type diagram was found, displaying a stoichiometry complexation of 2:1 (drug:host) and stability constant of 6494 ± 837 M−1. Stoichiometry was established by the UV spectrophotometer using Job’s plot method and, also confirmed by molecular modeling. Data from 1H-NMR, and FTIR, experiments also provided formation evidence of an inclusion complex between 4-NRC and HP-β-CD. 4-NRC complexation indeed led to higher drug solubility and stability which could probably be useful to improve its biological properties and make it available to oral administration and topical formulations.


4-Nerolidylcatechol 2-Hydroxypropyl-β-cyclodextrin Inclusion complex 1H-NMR and molecular dynamics 



This work was partly supported by a grant from CNPq and SECTEC-GO. The authors acknowledge Dr. Massayoshi Yoshida and Dr. Luiz Carlos Roque for the NMR 500 MHz data acquisition. We are especially grateful to Prof. Harry Pearson for English corrections on the manuscript.


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Líllian Amélia Soares
    • 1
  • Ana Flávia Vasconcelos Borges Leal
    • 1
  • Leonardo Fernandes Fraceto
    • 2
  • Elaine Rose Maia
    • 3
  • Inês Sabioni Resck
    • 3
  • Massuo Jorge Kato
    • 4
  • Eric de Sousa Gil
    • 1
  • Aparecido Ribeiro de Sousa
    • 5
  • Luiz Carlos da Cunha
    • 1
  • Kênnia Rocha Rezende
    • 1
  1. 1.Laboratório de Biofarmácia e Farmacocinética, Faculdade de FarmáciaUniversidade Federal de Goiás, Praça UniversitáriaGoiâniaBrazil
  2. 2.Departamento de Engenharia AmbientalUniversidade Estadual PaulistaSorocabaBrazil
  3. 3.Instituto de QuímicaUniversidade de BrasíliaBrasíliaBrazil
  4. 4.Departamento de Química Fundamental, Instituto de QuímicaUniversidade de São PauloSão PauloBrazil
  5. 5.Instituto de QuimicaUniversidade Federal de GoiásGoiâniaBrazil

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