Abstract
In present work the complexation of Res with two kinds of cyclodextrins (CDs), native β-cyclodextrin (β-CD) and modified hydroxypropyl-β-cyclodextrin (HP-CD), have been investigated by fluorescence spectroscopy, 1H-NMR spectroscopy and molecular modeling methods. The stoichiometric ratios, inclusion constants and thermodynamic parameters have been determined by the fluorescence data. In all cases 1:1 inclusion complexes are formed. The inclusion ability of HP-CD is larger than that of β-CD. Both inclusion processes have negative ∆G, negative ∆H and positive ∆S. Thermodynamic analysis suggests that Van der Waals force of guest-host interactions and the release of high-enthalpy water molecules from the cavity of CDs play important roles in driving complex formation. The study of molecular modeling shows that part of the A-ring and the B-ring of Res are placed in the cavity of β-CD, and the hydroxyl groups are projected outside. As for Res in HP-CD, the B-ring of Res is included in the cavity of HP-CD, and part of the A-ring is pointed outside. 1H-NMR spectroscopy results show that H2, H3, H4 and H5 protons of Res are more affected by the complexatin, indicating that they are located inside the torus of CDs, which are in agreement with the result of the molecular modeling.
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Fremont, L.: Biological effects of resveratrol. Life Sci. 66, 663–673 (2000). doi:10.1016/S0024-3205(99)00410-5
de la Lastra, C.A., Villegas, L.: Resveratrol as an anti-flammatory and anti-aging agent: mechanism and clinical implications. Mol. Nutr. Food Res. 49, 405–430 (2005). doi:10.1002/mnfr.200500022
Fremont, L., Belguendouz, L., Delpal, S.: Antioxidant activity of resveratrol and alcohol-free wine polyphenols related to LDL oxidation and polyunsaturated fatty acids. Life Sci. 64, 2511–2521 (1999). doi:10.1016/S0024-3205(99)00209-X
Brito, P., Almeida, L.M., Dinis, T.C.: The interaction of resveratrol with ferrylmyoglobin and peroxynitrite; protection against LDL oxidation. Free Radic. Biol. Med. 36, 621–631 (2002)
Pace-Asciak, C.R., Hahn, S., Diamandis, E.P., Soleas, G., Goldberg, D.M.: The red wine phenolics trans-resveratrol and quercetin block human platelet aggregation and eicosanoids synthesis: implications for protection against coronary heart disease. Clin. Chim. Acta 235, 207–219 (1995). doi:10.1016/0009-8981(95)06045-1
Jang, M., Cai, L., Udeani, G.O., Slowing, K.V., Thomas, C.F., Beecher, C.W., Fong, H.H., Farnsworth, N.R., Kinghorn, A.D., Mehta, R.G., Moon, R.C., Pezzuto, J.M.: Cancer chemopreventive activity of resveratrol, a natural product derived from grapes. Science 275, 218–220 (1997). doi:10.1126/science.275.5297.218
Athar, M., Back, J.H., Tang, X., Kim, K.H., Kopelovich, L., Bickers, D.R., Kim, A.L.: Resveratrol: a review of preclinical studies for human cancer prevention. Toxicol. Appl. Pharmacol. 224(3), 274–283 (2007). doi:10.1016/j.taap.2006.12.025
Brewster, M.E., Loftsson, T.: Cyclodextrins as pharmaceutical solubilizers. Adv. Drug Deliv. Rev. 59(7), 645–666 (2007). doi:10.1016/j.addr.2007.05.012
Calabrò, M.L., Tommasini, S., Donato, P., Raneri, D., Stancanelli, R., Ficarra, P., Ficarra, R., Costa, C., Catania, S., Rustichelli, C., Gamberini, G.: Effects of α- and β-cyclodextrin complexation on the physico-chemical properties and antioxidant activity of some 3-hydroxyflavones. J. Pharm. Biomed. 35, 365–377 (2004). doi:10.1016/j.jpba.2003.12.005
Lucas-Abellan, C., Fortea, I., Lopez-Nicolas, J.M., Nunez-Delicado, E.: Cyclodextrins as resveratrol carrier system. Food Chem. 104, 39–44 (2007). doi:10.1016/j.foodchem.2006.10.068
Jullian, C., Moyano, L., Yañez, C., Olea-Azar, C.: Complexation of quercetin with three kinds of cyclodextrins: an antioxidant study. Spectrochim. Acta [A] 67, 230–234 (2007). doi:10.1016/j.saa.2006.07.006
Alvariza, C., Usero, R., Mendicuti, F.: Binding of dimethyl 2, 3-naphthalenedicarboxylate with α-, β- and γ-cyclodextrins in aqueous solution. Spectrochim. Acta [A] 67, 420–429 (2007). doi:10.1016/j.saa.2006.07.039
Karathanos, V.T., Mourtzinos, I., Yannakopoulou, K., Andrikopoulos, N.K.: Study of the solubility, antioxidant activity and structure of inclusion complex of vanillin with β-cyclodextrin. Food Chem. 101, 652–658 (2007). doi:10.1016/j.foodchem.2006.01.053
Lu, Z., Cheng, B., Hu, Y.L., Zhang, Y.H., Zou, G.L.: Complexation of resveratrol with cyclodextrins:solubility and antioxidant activity. Food Chem. 113, 17–20 (2009). doi:10.1016/j.foodchem.2008.04.042
Bertacche, V., Lorenzi, N., Nava, D., Pini, E., Sinico, C.: Host-Guest interaction study of resveratrol with natural and modified cyclodextrins. J. Incl. Phenom. Macro. 55, 279–287 (2006). doi:10.1007/s10847-006-9047-8
Lucas-Abellán, C., Fortea, M.I., Gabaldón, J.A., Núñez-Delicado, E.: Complexation of resveratrol by native and modified cyclodextrins: determination of complexation constant by enzymatic, solubility and fluorimetric assays. Food Chem. 111, 262–267 (2008). doi:10.1016/j.foodchem.2008.03.073
López-Nicolás, J.M., García-Carmona, F.: Rapid, simple and sensitive determination of the apparent formation constants of trans-resveratrol complexes with natural cyclodextrins in aqueous medium using HPLC. Food Chem. 109, 868–875 (2008). doi:10.1016/j.foodchem.2008.01.022
Madrid, J.M., Mendicuti, F., Mattice, W.L.: Inclusion complexes of 2-methylnaphthoate and γ-cyclodextrin: experimental thermodynamics and molecular mechanics calculations. J. Phys. Chem. B 102, 2037–2044 (1998). doi:10.1021/jp9728870
Muthu Vijayan Enoch, I.V., Swaminathan, M.: Inclusion complexation of 2-amino-7-bromofluorene by β-cyclodextrin: spectral characteristics and the effect of pH. J. Fluoresc. 14, 751–756 (2004). doi:10.1023/B:JOFL.0000047226.60559.09
Mohammad, A., Naader, A.: Fluorescence enhancement of the aflatoxin B1 by forming inclusion complexes with some cyclodextrins and molecular modeling study. J. Lumin. 127, 575–582 (2007). doi:10.1016/j.jlumin.2007.03.012
Guo, Y.J., Chao, J.B., Pan, J.H.: Study on the interaction of 5-pyridine-10, 15, 20-tris-(p-chlorophenyl) porphyrin with cyclodextrins and DNA by spectroscopy. Spectrochim. Acta [A] 68, 231–236 (2007). doi:10.1016/j.saa.2006.11.019
Connors, K.A.: Binding Constants: The Measurement of Molecular Complex Stability. John Wiley and Sons, New York (1987)
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This work is supported by grants from National Fund of Nature Science of China (No.30670464) and the Open Research Fund of Hubei Novel Reactor & Green Chemical Technology Key Laboratory, Wuhan Institute of Technology (No. RGCT200805).
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Lu, Z., Chen, R., Liu, H. et al. Study of the complexation of resveratrol with cyclodextrins by spectroscopy and molecular modeling. J Incl Phenom Macrocycl Chem 63, 295–300 (2009). https://doi.org/10.1007/s10847-008-9522-5
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DOI: https://doi.org/10.1007/s10847-008-9522-5