Oxidation of Natural and Thermal Denatured Bovine Serum Albumin Hydrazyl Free Radicals in the Presence of Cyclodextrins



The influence of certain thermal treatments on the reactivity of bovine serum albumin (denaturation followed by renaturation in three different cooling conditions) was studied monitoring the kinetics of oxidation of BSA with two water-soluble stable hydrazyl radicals. The results showed that the addition to the reaction mixture of α-cyclodextrin and β-cyclodextrin decreases the oxidation rate, probably due to the encapsulation of terminal amino acid rests by the cyclodextrins cavity. β-Cyclodextrin protects more efficiently the albumin probes than α-cyclodextrin. The denatured albumin probes are more reactive than natural albumin as a consequence of the reorienting of the hydrophobic rests of albumin molecule to their surface.


BSA cyclodextrins hydrazyl radicals thermal denaturation 


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  1. He, X.M., Carter, D.C. 1992Nature358209Google Scholar
  2. Peters, T.,Jr. 1996All about AlbuminAcademic PressNew YorkGoogle Scholar
  3. Lehninger, A.L. 1975Biochemistry2Worth PublishersNYGoogle Scholar
  4. Ionita, G., Postolache, C., Tilimpea, C., Dinu, D., Sahini, V.E. 2003J. Plan. Chromatogr4308Google Scholar
  5. Ionita, G., Sahini, V.E. 2002Analele Universitatii BucurestiII153Google Scholar
  6. V.E. Sahini: 11th Physical Chemistry Conference, Timisoara, Romania, September 2–5 (2003).Google Scholar
  7. Barla, L., Tarsoaga, A., Sahini, V.E. 2003Analele Universitatii BucurestiI–II161Google Scholar
  8. Ionita, G., Sahini, V.E., Semenescu, Gh., Ionita, P. 2000Acta Chimica Slovenica47111Google Scholar
  9. Ioniţă, G., Ionita, P., Sahini, V.E., Luca , C. 2000J. Inclusion Phenom. Macrocyclic Chem.39269271Google Scholar
  10. Rohrbach, R.P., Rodriguez, I.J., Eyring, E., Wojik, J.F. 1977J. Phys. Chem81944Google Scholar
  11. Harata, K. 1981Bioorg. Chem10255Google Scholar
  12. Bertgson, R.J., Channing, M.A., McGovern, K.A. 1978J. Am. Chem. Soc1002878Google Scholar
  13. Nakajima, T., Sunagawa, M., Hirohashi, T., Fujoka, K. 1984Chem. Pharm. Bull32383Google Scholar
  14. Vanetten, R.L., Sebastian, J.F., Glowes, G.A., Bender, M.L. 1967J. Am. Chem. Soc893242Google Scholar
  15. Hardee, G.E., Otagiri, M., Perrin, J.H. 1978Acta. Pharm. Succ15188Google Scholar
  16. Schlenk, H., Sand, D.M. 1961J. Am. Chem. Soc832312Google Scholar
  17. K. Uekama, F. Hirayama, and T. Irie: Chem. Lett. 661 (1978).Google Scholar
  18. Armstrong, D.W., Nome, F., Spino, L.A., Golden, T.D. 1986J. Am. Chem. Soc1081418Google Scholar
  19. K. Fujimura, T. Veda, M. Kitagawa, H. Takayanagi, and T. Ando: Anal. Chem. 2668 (1986).Google Scholar
  20. Nguyen, T.P., Turme, M., Lettelier, P., Gosselet, N.M., Sebille, B. 1997Pharm21653Google Scholar
  21. Takahashi, K. 1998Chem. Rev982013Google Scholar
  22. Putirszkaja, G.V., Siladi, T. 1972Acta Chim. Hung72329Google Scholar
  23. D. Laloo and M.K. Mahanti: J. Chem. Soc. Dalton Trans. 311 (1990).Google Scholar
  24. Matsubara, K., Irie, T., Uekama, K. 1997Chem. Pharm. Bull45378Google Scholar
  25. Katakam, M., Banga, A.K. 1995J. Pharm. Pharmacol47103Google Scholar
  26. Prankerd, R.J., Stone, H.W., Dloan, K.B., Perrin, J.H. 1992Int. J. Pharm88189Google Scholar
  27. Camilleri, P., Haskins, N.J., Howlett, D.R. 1994FEBS Lett341256Google Scholar

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© Kluwer Academic Publishers 2004

Authors and Affiliations

  1. 1.Institute of Physical ChemistryBucharestRomania
  2. 2.Faculty of ChemistryUniversity of BucharestBucharestRomania

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