Common genetic variants in selected Ca2+ signaling genes and the risk of appropriate ICD interventions in patients with heart failure

  • Pietro Francia
  • Carmen Adduci
  • Agnese Ricotta
  • Rosita Stanzione
  • Isabella Sensini
  • Arianna Uccellini
  • Alessandra Frattari
  • Cristina Balla
  • Maria Cotugno
  • Riccardo Cappato
  • Speranza Rubattu
  • Massimo Volpe



Defective Ca2+ handling in failing cardiomyocites predisposes patients with heart failure (HF) to ventricular arrhythmia. We investigated whether gene variants of Ca2+ handling proteins are associated with the occurrence of ventricular tachycardia/fibrillation (VT/VF) in HF patients implanted with a primary prevention implantable cardioverter-defibrillator (ICD).


One hundred thirty-six patients with HF were followed from ICD implantation to the time of first appropriate ICD intervention for VT > 170 bpm. The following polymorphisms were genotyped: ATP2A2 rs1860561 and rs56243033; RYR2 rs4142933; CASQ2 rs4074536; SLC8A1 g.-23449C > A; PLN rs12198461; FKBP1B rs7568163. Hazard ratios (HR) were derived from Cox proportional-hazards regression analysis.


After a mean follow-up of 879 days (IQ range, 327 to 1,459), 34 patients (25 %) had appropriate ICD intervention. Non-sustained VT (HR, 2.12; 95 %CI, 0.87–5.19; p = 0.09) and atrial fibrillation (AF) at ICD implantation (HR, 2.33; 95 %CI, 0.89–6.10; p = 0.08) predicted appropriate ICD interventions with borderline statistical significance. Prevalence of ATP2A2 rs1860561 variant was 17 % in patients without VT/VF and 4 % in those with ventricular arrhythmia (p = 0.009). After adjustment for AF and NSVT, the rs1860561 A mutant allele independently predicted lower incidence of VT/VF (HR, 0.29; 95 %CI, 0.09–0.98; p = 0.04).


The observation that ATP2A2 rs1860561 gene variant associated with lower risk of life-threatening arrhythmia in HF patients suggests that selected calcium gene variants may modify the risk of SD even within the complex and polygenic pathological condition of HF. Combining traditional risk factors and genetic profiling could reveal helpful to identify HF patients who will benefit most from ICD implantation.


Sudden death Heart failure Gene variants Calcium handling proteins ICD 


Sources of funding

This work was supported by a grant (Ricerca Corrente) from the Italian Ministry of Health to MV and SR; by the 5 ‰ grant to MV and SR; by PRIN 2009 to SR; by Ingenious HyperCare European Project to MV; by a grant from University Sapienza to PF.

Conflicts of interest

None declared


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Pietro Francia
    • 1
  • Carmen Adduci
    • 1
  • Agnese Ricotta
    • 1
  • Rosita Stanzione
    • 2
  • Isabella Sensini
    • 1
  • Arianna Uccellini
    • 1
  • Alessandra Frattari
    • 1
  • Cristina Balla
    • 1
  • Maria Cotugno
    • 2
  • Riccardo Cappato
    • 3
  • Speranza Rubattu
    • 1
    • 2
  • Massimo Volpe
    • 1
    • 2
  1. 1.Division of Cardiology, Department of Clinical and Molecular MedicineSapienza University of Rome Sant’Andrea HospitalRomeItaly
  2. 2.I.R.C.C.S. NeuromedPozzilliItaly
  3. 3.Arrhythmia and Electrophysiology CentreI.R.C.C.S. San DonatoSan Donato MilaneseItaly

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