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Genome-wide screening differential long non-coding RNAs expression profiles discloses its roles involved in OHSS development

  • Genetics
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Objective

To screen differentially expressed lncRNAs involved in OHSS. OHSS is defined as ovarian hyperstimulation syndrome. It is characterized as enlarged ovary and increased vascular permeability.

Design

Case-control study.

Setting

University-affiliated hospital.

Patient(s)

Patients with OHSS high risk (n = 30) and low risk (n = 30) were included in this study.

Intervention(s)

None.

Main outcome measure(s)

LncRNAs from women with OHSS high risk and low risk were used for high-throughput sequencing profiling. The eight most differentially expressed lncRNAs in granulosa cells were validated by semi-quantitative reverse transcription-polymerase chain reaction analysis.

Result(s)

A total of 23,815 lncRNAs were detected and 482 were differentially expressed (fold-change ≥2; p < 0.05, FDR value < 0.001), of which 205 were upregulated and 277 were downregulated. Lnc-SEC16B.1-6, lnc-SNURF-13, lnc-LGR6-6, and lnc-H2AFY2-2 were up-regulated, while lnc-BRD2-2, lnc-HSPA6-2, and lnc-CLIC6-5 were downregulated significantly in granulosa cells. These results were confirmed by qRT-PCR. KEGG pathways and Gene Ontology enrichment analysis revealed that several biological processes were significantly associated. Meanwhile, the lncRNA/miRNA interaction network was established according to ceRNA network model.

Conclusion(s)

Comprehensive expression screening identified eight novel lncRNAs associated with risk factors of OHSS process. Although it is unclear how these altered lncRNAs regulate the process of OHSS, our findings suggest these lncRNAs may be novel players in OHSS development.

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Acknowledgements

This study was funded by National Natural Science Funding (no. 81701519).

Author information

Authors and Affiliations

  1. Department of Gynecology and Obstetrics, Sun Yat-Sen Memorial Hospital, Guangzhou, China

    Haiyan Lin, Yu Li, Qi Qiu, Wenjun Wang & Qingxue Zhang

  2. Department of Gynecology and Obstetrics, The third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China

    Weijie Xing

Authors
  1. Haiyan Lin
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  2. Yu Li
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  3. Weijie Xing
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  4. Qi Qiu
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  5. Wenjun Wang
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  6. Qingxue Zhang
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Correspondence to Qingxue Zhang.

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Lin, H., Li, Y., Xing, W. et al. Genome-wide screening differential long non-coding RNAs expression profiles discloses its roles involved in OHSS development. J Assist Reprod Genet 35, 1473–1482 (2018). https://doi.org/10.1007/s10815-018-1199-0

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  • DOI: https://doi.org/10.1007/s10815-018-1199-0

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