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Histone demethylase KDM4A and KDM4B expression in granulosa cells from women undergoing in vitro fertilization

  • Reproductive Physiology and Disease
  • Published:
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Abstract

Purpose

To assess expression of the histone demethylases KDM4A and KDM4B in granulosa collected from women undergoing oocyte retrieval and to determine if expression was related to pregnancy outcome.

Methods

Cumulus and mural granulosa cells were obtained from women undergoing oocyte retrieval. KDM4A and KDM4B mRNA expression was determined by qRT-PCR. KDM4A and KDM4B proteins were immunohistochemically localized in ovarian tissue sections obtained from archival specimens.

Results

KDM4A and KDM4B protein was localized to oocytes, granulosa cells, and theca and luteal cells in ovaries from reproductive-aged women. KDM4A and KDM4B mRNA expression was overall higher in cumulus compared to mural granulosa. When comparing granulosa demethylase gene expression, KDM4A and KDM4B mRNA expression was higher in both cumulus and mural granulosa from not pregnant patients compared to patients in the pregnant-live birth group.

Conclusions

Histone demethylases KDM4A and KDM4B mRNA are differentially expressed in cumulus and mural granulosa. Expression of both KDM4A and KDM4B mRNA was lower in cumulus granulosa and mural granulosa from pregnant compared to not pregnant patients. These findings suggest that altered expression of histone demethylases may impact epigenetic changes in granulosa cells associated with pregnancy.

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Author notes

  1. Adam J. Krieg

    Present address: Department of Obstetrics and Gynecology, Oregon Health and Science University, Portland, OR, USA

  2. Adam J. Krieg & Sacha A. Krieg

    Present address: Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Beaverton, OR, 97006, USA

Authors and Affiliations

  1. Institute for Reproductive Health and Regenerative Medicine, Center for Reproductive Sciences, University of Kansas Medical Center, 3901 Rainbow Blvd. MS3050, Kansas City, KS, 66160, USA

    Adam J. Krieg, Sacha A. Krieg & Katherine F. Roby

  2. Women’s Health Specialty Center Advanced Reproductive Medicine, University of Kansas Medical Center, Kansas City, KS, 66160, USA

    Sacha A. Krieg & Katherine F. Roby

  3. Department of Obstetrics and Gynecology, University of Kansas Medical Center, Kansas City, KS, 66160, USA

    Adam J. Krieg, Frances Grimstad, Kaitlin Marquis, Elizabeth Constance, Yan Hong & Sacha A. Krieg

  4. Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS, 66160, USA

    Adam J. Krieg

  5. Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS, 66160, USA

    Sarah R. Mullinax & Katherine F. Roby

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Krieg, A.J., Mullinax, S.R., Grimstad, F. et al. Histone demethylase KDM4A and KDM4B expression in granulosa cells from women undergoing in vitro fertilization. J Assist Reprod Genet 35, 993–1003 (2018). https://doi.org/10.1007/s10815-018-1151-3

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