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Fertility preservation for trans men: frozen-thawed in vitro matured oocytes collected at the time of ovarian tissue processing exhibit normal meiotic spindles

  • Fertility Preservation
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Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purposes

At the moment of sex reassignment surgery (SRS), the ovarian tissue is sometimes cryopreserved as fertility preservation option for female-to-male trans men, also called trans men. During this preparation, cumulus-oocyte-complexes (COCs) can be found and in vitro matured. It is not known if these oocytes are developmentally competent. In order to use these oocytes for fertility preservation and subsequent fertilization, a normal spindle structure before and after vitrification is necessary.

Methods

A total of 680 COCs were collected from trans men (n = 16) at the time of SRS and after testosterone treatment. The COCs were subjected to in vitro maturation and those that reached the metaphase II stage (MII) were collected and split into two groups; group 1 was immediately fixed for spindle staining and group 2 was first vitrified and warmed followed by spindle staining. Statistical analysis was performed by Fisher’s exact test.

Results

After 48 h in vitro maturation, 38.1% of COCs were at MII stage. Those oocytes were split in two groups: (1) 126 MII oocytes in the noncryopreservation group and (2) 133 MII oocytes underwent cryopreservation through vitrification. The oocyte survival rate, after 2 h warming, was 67.7%. Both the noncryopreserved and the vitrified group showed comparable results concerning normal spindle structure and chromosomes alignment, 85.7% vs. 92.2% (P = 0.27).

Conclusions

Spindle structure analysis and chromosomal alignment after vitrification seem normal in in vitro matured COCs collected during the tissue processing of ovaries in trans men at the time of SRS. The MII oocytes do not seem to be morphologically affected by prolonged testosterone treatment.

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References

  1. De Sutter P, Kira K, Verschoor A, Hotimsky A. The desire to have children and the preservation of fertility in transsexual women: a survey. International Journal of Transgenderism. 2002;6:3.

    Google Scholar 

  2. De Roo C, Tilleman K, T'Sjoen G, De Sutter P. Fertility options in transgender people. Int Rev Psychiatry. 2016;28:112–9.

    Article  PubMed  Google Scholar 

  3. Wierckx K, Van Caenegem E, Pennings G, Elaut E, Dedecker D, Van de Peer F, et al. Reproductive wish in transsexual men. Hum Reprod. 2012;27:483–7.

    Article  PubMed  Google Scholar 

  4. Van den Broecke R, Van der Elst J, Liu J, Hovatta O, Dhont M. The female-to-male transsexual patient: a source of human ovarian cortical tissue for experimental use. Hum Reprod. 2001;16:145–7.

    Article  Google Scholar 

  5. Telfer EE, McLaughlin M. In vitro development of ovarian follicles. Semin Reprod Med. 2011;29:15–23.

    Article  CAS  PubMed  Google Scholar 

  6. Telfer EE, McLaughlin M. Strategies to support human oocyte development in vitro. Int J Dev Biol. 2012;56:901–7.

    Article  CAS  PubMed  Google Scholar 

  7. Telfer EE, McLaughlin M, Ding C, Thong KJ. A two-step serum-free culture system supports development of human oocytes from primordial follicles in the presence of activin. Hum Reprod. 2008;23:1151–8.

    Article  CAS  PubMed  Google Scholar 

  8. Huang JY, Tulandi T, Holzer H, Tan SL, Chian RC. Combining ovarian tissue cryo banking with retrieval of immature oocytes followed by in vitro maturation and vitrification: an additional strategy of fertility preservation. Fertil Steril. 2008;89:567–72.

    Article  PubMed  Google Scholar 

  9. Fasano G, Moffa F, Dechène J, Englert Y, Demeestere I. Vitrification of in vitro matured oocytes collected from antral follicles at the time of ovarian tissue cryopreservation. Reprod Biol and endocrinology. 2011;9:150.

    Article  CAS  Google Scholar 

  10. Wilken-Jensen HN, Kristensen SG, Jeppesen JV, Andersen CY. Developmental competence of oocytes isolated from surplus medulla tissue in connection with cryopreservation of ovarian tissue for fertility preservation. ACTA Obstetricia et Gynecologica Scnadinavica. 2013;93:32–7.

    Article  Google Scholar 

  11. Segers I, Mateizel I, Van Moer E, Smitz J, Tournaye H, Verheyen G, et al. In vitro maturation (IVM) of oocytes recovered from ovarectomy specimens in the laboratory: a promising “ex vivo” method of oocyte cryopreservation resulting in the first report of an ongoing pregnancy in Europe. J Assist Reprod Genet. 2015;32:1221–31.

    Article  PubMed  PubMed Central  Google Scholar 

  12. Abir R, Ben-Aharon I, Garor R, Yaniv I, Ash S, Stemmer SM, et al. Cryopreservation of in vitro matured oocytes in addition to ovarian tissue freezing for fertility preservation in paediatric female cancer patients before and after cancer therapy. Hum Reprod. 2016;31:750–62.

    Article  CAS  PubMed  Google Scholar 

  13. Yin H, Kristensen SG, Jiang H, Rasmussen A, Yding Andersen C. Survival and growth of isolated pre-antral follicles from human ovarian medulla tissue during long-term 3D culture. Hum Reprod. 2016;31:1531–9.

    Article  CAS  PubMed  Google Scholar 

  14. De Roo C, Lierman S, Tilleman K, Peynshaert K, Braeckmans K, Caanen M, Lambalk CB, Weyers S, T’Sjoen G, Cornelissen R, De Sutter P. Ovarian tissue cryopreservation in female-to-male transgender people: insights into ovarian histology and physiology after prolonged androgen treatment. RBM Online. 2017. doi:10.1016/j.rbmo.2017.03.008.

  15. Nikiforaki D, Vanden Meerschaut F, Qian C, De Croo I, Lu Y, Deroo T, et al. Oocyte cryopreservation and in vitro culture affect calcium signalling during human fertilization. Hum Reprod. 2014;29(1):29–40.

    Article  CAS  PubMed  Google Scholar 

  16. Mattson BA, Albertini DF. Oogenesis: chromatin and microtubule dynamics during meiotic prophase. Mol Reprod Dev. 1990;25:374–83.

    Article  CAS  PubMed  Google Scholar 

  17. Combelles CMH, Ceyhan ST, WangH RC. Maturation outcome are improved following cryoleaf vitrification of immature human oocytes when compared to choline-based slow-freezing. J Assist Reprod Genet. 2011;28:1183–92.

    Article  PubMed  PubMed Central  Google Scholar 

  18. Fasano G, Dechène J, Antonacci R, Biramane J, Vannin AS, Van Langendonckt A, et al. Outcomes of immature oocytes collected from ovarian tissue for cryopreservation in adult and prepubertal patients. Reprod BioMed Online. 2017; doi:10.1016/j.rbmo.2017.03.007.

  19. Shalom-Paz E, Almong B, Shehata F, Huang J, Holzer H, Chian RC, et al. Fertility preservation for breast-cancer patients using IVM followed by oocyte or embryo vitrification. Reprod BioMed Online. 2010;21:566–71.

    Article  PubMed  Google Scholar 

  20. Mandelbaum J, Anastasiou O, Lévy R, Guérin JF, de Larouzière V, Antoine JM. Effects of cryopreservation on the meiotic spindle of human oocytes. Eur J Obstet Gynecol Reprod Biol. 2004;5(113 Suppl 1):S17–23.

    Article  Google Scholar 

  21. Coticchio G, Bromfield JJ, Sciajno R, Gambardella A, Scaravelli G, Borini A, et al. Vitrification may increase the rate of chromosome misalignment in the metaphase II spindle of human mature oocytes. Reprod BioMed Online. 2009;19(3):29–34.

    Article  PubMed  Google Scholar 

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Acknowledgments

This work is supported by the Fonds voor Wetenschappelijk Onderzoek (FWO) TBM T001816 N and the Gender Identity Research and Education Society (GIRES) foundation.

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Authors and Affiliations

  1. Department for Reproductive Medicine, Ghent University Hospital, De Pintelaan 185, 9000, Ghent, Belgium

    S. Lierman, K. Tilleman & P. De Sutter

  2. Department of Pharmaceutics, Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Harelbekestraat 72, 9000, Ghent, Belgium

    K. Braeckmans & K. Peynshaert

  3. Department of Gynaecology, Ghent University Hospital, De Pintelaan 185, 9000, Ghent, Belgium

    S. Weyers

  4. Department of Endocrinology and Centre for Sexuoloy and Gender, De Pintelaan 185, 9000, Ghent, Belgium

    G. T’Sjoen

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  1. S. Lierman
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  2. K. Tilleman
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  3. K. Braeckmans
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  4. K. Peynshaert
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  5. S. Weyers
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  6. G. T’Sjoen
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  7. P. De Sutter
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Correspondence to S. Lierman.

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Lierman, S., Tilleman, K., Braeckmans, K. et al. Fertility preservation for trans men: frozen-thawed in vitro matured oocytes collected at the time of ovarian tissue processing exhibit normal meiotic spindles. J Assist Reprod Genet 34, 1449–1456 (2017). https://doi.org/10.1007/s10815-017-0976-5

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  • DOI: https://doi.org/10.1007/s10815-017-0976-5

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