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The MDM2 promoter T309G polymorphism was associated with preeclampsia susceptibility

  • Genetics
  • Published:
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Abstract

Purpose

Preeclampsia (PE) is a hypertensive disorder of pregnancy in which abnormal proliferation and apoptosis of placenta trophoblast has a pivotal role in its pathophysiology. The aim of the current study was to examine the association between Mouse Double Minute 2 (MDM2) T309G and 40 bp insertion/deletion (I/D) polymorphisms and PE risk.

Methods

A case-control study was conducted on 208 PE women and 164 healthy pregnant women matching age, sex, and ethnicity. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and PCR methods were used for genotyping.

Results

The MDM2 309GG genotype was associated with PE, and this genotype was found to be a risk factor for PE. There was no association between the MDM2 I/D polymorphism and PE. The haplotype-based association analysis revealed no association between MDM2 T309G and 40 bp I/D polymorphisms and PE. The frequency of TT-DD and GG-DD combined genotypes were significantly higher in PE women with marginal P values (P = 0.046).

Conclusions

The MDM2 309GG genotype was associated with higher risk of PE. The TT-DD and GG-DD combined genotypes were higher in PE women.

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Acknowledgements

The study was supported by the Research Center of Research Deputy in Zahedan University of Medical Sciences (Registered No. 1395.18).

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Authors and Affiliations

  1. Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

    Saeedeh Salimi, Abbas Mohammadpour-Gharehbagh, Mahnaz Rezaei & Mojtaba Sajadian

  2. Department of Clinical Biochemistry, School of Medicine and Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

    Saeedeh Salimi, Abbas Mohammadpour-Gharehbagh, Mahnaz Rezaei & Mojtaba Sajadian

  3. Department of Obstetrics and Gynecology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

    Batool Teimoori, Atefeh Yazdi & Mojgan Mokhtari

  4. Department of Obstetrics and Gynecology, School of Medicine, Shahid Beheshty University of Medical Sciences, Tehran, Iran

    Minoo Yaghmaei

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  1. Saeedeh Salimi
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  2. Abbas Mohammadpour-Gharehbagh
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Corresponding author

Correspondence to Abbas Mohammadpour-Gharehbagh.

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Informed consent was obtained from all subjects, and the study protocol was approved by the Ethics Committee of Zahedan University of Medical Sciences and conducted in accordance with the Declaration of Helsinki.

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Salimi, S., Mohammadpour-Gharehbagh, A., Rezaei, M. et al. The MDM2 promoter T309G polymorphism was associated with preeclampsia susceptibility. J Assist Reprod Genet 34, 951–956 (2017). https://doi.org/10.1007/s10815-017-0941-3

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  • DOI: https://doi.org/10.1007/s10815-017-0941-3

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